Total polyphenol of <i><scp>A</scp>nemarrhena asphodeloides</i> ameliorates advanced glycation end products‐induced endothelial dysfunction by regulation of <scp>AMP</scp>‐<scp>K</scp>inase (知母多酚通过激活AMP‐激酶来缓解糖基化终末产物引起的内皮损伤)

Zhao, Qianwen; Sun, Yan; Ji, Yu; Xu, Lian; Liu, Kang; Liu, Baolin; Huang, Fang

doi:10.1111/1753-0407.12111

Cited by 9 publications

(6 citation statements)

References 35 publications

(59 reference statements)

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“…EXO-FBS500A-1; System Biosciences, Palo Alto, CA, USA). According to our previous study (21), the PVAT samples were cultured with PA (100 µM) in the presence or absence of mangiferin (0.1, 1 or 10 µM) for 2 h; samples cultured without PA or mangiferin were used as the control group. Subsequent to washing with PBS, PVAT samples were incubated in DMEM for a further 22 h. The supernatant was then collected as the conditioned medium (CM), and larger particles were removed by centrifugation at 10,000 × g for 5 min at 4°C and filtration through a 0.22-µm filter.…”

Section: Methodsmentioning

confidence: 99%

Exosomes derived from mangiferin‑stimulated perivascular adipose tissue ameliorate endothelial dysfunction

et al. 2019

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Perivascular adipose tissue (PVAT) is considered to serve a vital role during the development of endothelial dysfunction. The current study investigated the effect of exosomes derived from mangiferin-stimulated PVAT on endothelial function, including regeneration, migration, apoptosis and inflammation. The number of exosomes secreted by PVAT was increased by stimulation with mangiferin (0.1, 1 or 10 µM), and uptake of these exosomes by endothelial cells was observed. Exosomes produced by stimulation of PVAT with mangiferin reversed the effects of inflammation-induced endothelial dysfunction following palmitic acid (PA) treatment. Furthermore, nuclear factor (NF)-κB signaling in endothelial cells was significantly increased when treated with PA-induced PVAT-derived exosomes, whereas exosomes from the supernatant of PVAT stimulated with mangiferin reduced p65 and p50 phosphorylation levels in the cells, and inhibited p65 transportation to the nucleus. Taken together, the present study demonstrated that exosomes derived from mangiferin-stimulated PVAT supernatant inhibited inflammation-induced endothelial dysfunction via modulation of NF-κB signaling.

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Section: Methodsmentioning

confidence: 99%

Exosomes derived from mangiferin‑stimulated perivascular adipose tissue ameliorate endothelial dysfunction

et al. 2019

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“…Mangiferin regulates AMP-Kinase (AMPK) activity and this action is involved in amelioration of metabolic disorders [19,20]. Our previous works also showed that mangiferincontaining total polyphenol of Anemarrhena asphodeloides ameliorated endothelial dysfunction with regulation of AMPK activity [21]. AMPK is an energy sensor with antiinflammatory activity.…”

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confidence: 93%

Mangiferin inhibits endoplasmic reticulum stress-associated thioredoxin-interacting protein/NLRP3 inflammasome activation with regulation of AMPK in endothelial cells

Song

Hou

et al. 2015

Metabolism

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“…The rhizomes of A. asphodeloides , known as Zhimu ( Anemarrhenae Rhizoma ), have been widely used in the treatment of febrile diseases, hyperpyrexia, polydipsia, cough, osteopyrexia, fever and constipation since ancient times (Chinese Pharmacopoeia, ). Modern pharmacological studies have shown that A. asphodeloides has obvious effects including anti‐tumor (Guo et al, ), anti‐oxidation (Chae et al, ), anti‐inflammation (Bak et al, ) and anti‐diabetes (Zhao et al, ). Meanwhile, it has exhibited neuroprotective activities for central nervous system disorders such as Alzheimer's and Parkinson's disease (Lee, Jung, & Kim, ; Won & Ma, ).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a UPLC–MS/MS method for determination of Sarsasapogenin‐AA22 in rat plasma and its application to a pharmacokinetic study

Pei

et al. 2018

Biomedical Chromatography

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A sarsasapogenin derivative, sarsasapogenin-AA22 (AA22), with cyclobutylamine at the 3-hydroxyl position of sarsasapogenin, has great neuroprotective activity in PC12 cells and NO production inhibitory activity in RAW264.7 cell lines. A method was developed to determine AA22 in rat plasma which was further applied to evaluate the pharmacokinetics of AA22 after taking a single dose of AA22. Liquid chromatography tandem mass spectrometry was used in the method, while diosgenin was used as internal standard. A simple protein precipitation based on acetonitrile was utilized. A simple sample cleanup promoted the throughput of the method considerably. The method was validated over the range of 1-1000 ng/mL with a correlation coefficient > 0.99. The lower limit of quantification was 1 ng/mL for AA22 in plasma. Intra- and inter-day accuracies for AA22 were 92-111 and 100-103%, respectively, and the inter-day precision was <15%. After a single oral dose of 25 mg/kg of AA22, the mean peak plasma concentration of AA22 was 2114 ± 362 ng/mL at 6 h. The area under the plasma concentration-time curve was 196,098 ± 69,375 h ng/mL, and the elimination half-life was 8.7 ± 2.2 h.

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Total polyphenol of Anemarrhena asphodeloides ameliorates advanced glycation end products‐induced endothelial dysfunction by regulation of AMP‐Kinase (知母多酚通过激活AMP‐激酶来缓解糖基化终末产物引起的内皮损伤)

Abstract: Total polyphenol of Anemarrhena asphodeloides inhibited AGEs-induced ROS-associated inflammation and ameliorated endothelial dysfunction through beneficial regulation of AMPK activation.

Cited by 9 publications

References 35 publications

Exosomes derived from mangiferin‑stimulated perivascular adipose tissue ameliorate endothelial dysfunction

Exosomes derived from mangiferin‑stimulated perivascular adipose tissue ameliorate endothelial dysfunction

Mangiferin inhibits endoplasmic reticulum stress-associated thioredoxin-interacting protein/NLRP3 inflammasome activation with regulation of AMPK in endothelial cells

Development and validation of a UPLC–MS/MS method for determination of Sarsasapogenin‐AA22 in rat plasma and its application to a pharmacokinetic study

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