Total Phenols and Flavonoids Content, Antioxidant Capacity and Lipase Inhibition of Root and Leaf Horseradish (&amp;lt;i&amp;gt;Armoracia rusticana&amp;lt;/i&amp;gt;) Extracts

Calabrone, Luana; Larocca, Marilena; Marzocco, Stefania; Martelli, Giuseppe; Rossano, Rocco

doi:10.4236/fns.2015.61008

Cited by 23 publications

(18 citation statements)

References 31 publications

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“…Some of the recognized properties of PCs include antioxidant [ 6 ], antiproliferative [ 7 ], antibacterial [ 8 ], antiviral [ 9 ], anti-inflammatory [ 1 ], antidiabetic [ 10 ] anti-obesity activity [ 11 ], and others. However, the antidiabetic and anti-obesity activities related to digestive enzymes are the focus of this work.…”

Section: Introductionmentioning

confidence: 99%

Polyphenolic Compounds and Digestive Enzymes: In Vitro Non-Covalent Interactions

et al. 2017

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The digestive enzymes–polyphenolic compounds (PCs) interactions behind the inhibition of these enzymes have not been completely studied. The existing studies have mainly analyzed polyphenolic extracts and reported inhibition percentages of catalytic activities determined by UV-Vis spectroscopy techniques. Recently, pure PCs and new methods such as isothermal titration calorimetry and circular dichroism have been applied to describe these interactions. The present review focuses on PCs structural characteristics behind the inhibition of digestive enzymes, and progress of the used methods. Some characteristics such as molecular weight, number and position of substitution, and glycosylation of flavonoids seem to be related to the inhibitory effect of PCs; also, this effect seems to be different for carbohydrate-hydrolyzing enzymes and proteases. The digestive enzyme–PCs molecular interactions have shown that non-covalent binding, mostly by van der Waals forces, hydrogen binding, hydrophobic binding, and other electrostatic forces regulate them. These interactions were mainly associated to non-competitive type inhibitions of the enzymatic activities. The present review emphasizes on the digestive enzymes such as α-glycosidase (AG), α-amylase (PA), lipase (PL), pepsin (PE), trypsin (TP), and chymotrypsin (CT). Existing studies conducted in vitro allow one to elucidate the characteristics of the structure–function relationships, where differences between the structures of PCs might be the reason for different in vivo effects.

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Section: Introductionmentioning

confidence: 99%

Polyphenolic Compounds and Digestive Enzymes: In Vitro Non-Covalent Interactions

et al. 2017

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“…Rivelli et al reported a high content in vitamin C in both roots and leaves of horseradish [27], compound with strong antioxidant activity which was used as reference in our experiment. The antioxidant activity of horseradish roots and leaves was also assessed by Calabrone et al who revealed that hydroalcoholic extracts exhibited a stronger DPPH radical scavenging activity than methanolic ones and attributed it to the higher content in phenolic compounds; also the main phenolic compounds are represented by flavonoids [28]. The results reported in the current paper are in line with those reported in a pilot study by Biller et al who calculated that 1 g dry substance of horseradish exhibited an antioxidant potential equal to 4.31 to 8.78 mg of vitamin C [29].…”

Section: 1antioxidant Activitymentioning

confidence: 99%

Hydro-alcoholic Extracts of Roots and Sprouts of Armoracia rusticana: Assessment of Antioxidant and Cytotoxic Activity

Racoviceanu¹,

Farcaş²,

Ghiulai³

et al. 2020

Rev. Chim.

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Armoracia rusticana � horseradish is a well-known vegetable used from ancient times and consumed nowadays more for its taste than its pharmacological properties, even though it proved to be a good agent in fighting several health problems. This study focuses on a comparative assessment of several horseradish hydro-alcoholic extracts, obtained from roots and sprouts, respectively, mainly evaluating the antioxidant activity but also a potential cytotoxic effect. The DPPH evaluation revealed that horseradish extracts made from roots or sprouts exhibit a strong antioxidant activity compared to the ascorbic acid used as etalon. The in vitro evaluation established that a significant inhibitory effect on cell viability and morphology of MDA-MB-231 � breast adenocarcinoma cell line was displayed by the hydroalcoholic extracts in a dose-dependent manner.

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“…Los flavonoides, el grupo más amplio de polifenoles, han sido ampliamente estudiados por su actividad antioxidante, antimicrobiana, antiinflamatoria, entre otras actividades biológicas (Li et al, 2014;Calabrone et al, 2015). En años recientes se ha estudiado su interacción con proteínas, como las enzimas digestivas para evaluar su posible uso en el control de la obesidad y diabetes (Li et al, 2011;Martinez-Gonzalez et al, 2017, 2019.…”

Section: Introductionunclassified

Inhibición de lipasa pancreática por flavonoides: importancia del doble enlace C2=C3 y la estructura plana del anillo C//Inhibition of pancreatic lipase by flavonoids: relevance of the C2=C3 double bond and C-ring planarity

et al. 2020

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Lipasa pancreática es una enzima clave en el metabolismo de lípidos. Los flavonoides son compuestos bioactivos de gran relevancia debido a sus interacciones con enzimas digestivas. Se evaluó la actividad de lipasa pancreática en presencia de flavonoides. Mediante espectroscopía UVVisible se determinó que el mejor inhibidor fue quercetina, seguido de rutina > luteolina > catequina > hesperetina, con valores de IC50 de 10.30, 13.50, 14.70, 28.50 y 30.50 μM, respectivamente. Todos los flavonoides mostraron una inhibición mixta, excepto catequina que mostró una inhibición acompetitiva. La capacidad inhibitoria de los flavonoides se relacionó con propiedades estructurales compartidas entre los distintos flavonoides, como la hidroxilación en las posiciones C5, C7 (anillo A), C2’ y C3’ (anillo B), y el doble enlace entre C2 y C3 (anillo C). Los resultados de inhibición coincidieron con el análisis de la fluorescencia extrínseca. Los estudios de docking molecular indicaron que la interacción entre lipasa pancreática y los flavonoides fue principalmente mediante interacciones hidrofóbicas (pi-stacking). Las interacciones de todos los flavonoides, excepto rutina, se dieron en el mismo sitio (subsitio 1) de la enzima. La insaturación entre C2 y C3 fue determinante para el acomodo de los flavonoides con la enzima, principalmente por interacciones de pi-stacking.ABSTRACTPancreatic lipase is a key enzyme in lipid metabolism. Flavonoids are bioactive compounds obtained from vegetables with big relevance, due to their intrinsic interaction with digestive enzymes. Pancreatic lipase activity was evaluated in the presence of flavonoids, through UV-Vis spectroscopy. All tested flavonoids showed a mixed-type inhibition, except catechin, which showed a uncompetitive inhibition. The best inhibitor was quercetin followed by rutin > luteolin > catechin > hesperetin, with IC50 values of 10.30, 13.50, 14.70, 28.50 and 30.50 μM, respectively. The flavonoids inhibitory capacity was related to structural properties shared between the different flavonoids, such as the hydroxylation at C5, C7 (ring A), C2’ and C3’ (ring B), and the double bond between C2 and C3 (ring C). The inhibition results are in agreement with the extrinsic fluorescence analysis. Molecular docking studies indicated that the interaction between pancreatic lipase and flavonoids was mainly through hydrophobic interactions (pi-stacking). The interactions of all flavonoids, except rutin, occurred at the same enzyme site (subsite 1). Instauration between C2 and C3 was decisive for the arrangement of flavonoids with the enzyme, mainly due to pi-stacking interactions.

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Total Phenols and Flavonoids Content, Antioxidant Capacity and Lipase Inhibition of Root and Leaf Horseradish (<i>Armoracia rusticana</i>) Extracts

Cited by 23 publications

References 31 publications

Polyphenolic Compounds and Digestive Enzymes: In Vitro Non-Covalent Interactions

Polyphenolic Compounds and Digestive Enzymes: In Vitro Non-Covalent Interactions

Hydro-alcoholic Extracts of Roots and Sprouts of Armoracia rusticana: Assessment of Antioxidant and Cytotoxic Activity

Inhibición de lipasa pancreática por flavonoides: importancia del doble enlace C2=C3 y la estructura plana del anillo C//Inhibition of pancreatic lipase by flavonoids: relevance of the C2=C3 double bond and C-ring planarity

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