Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal

Abstract

Eight selected wild vegetables from Nepal (Alternanthera sessilis, Basella alba, Cassia tora, Digera muricata, Ipomoea aquatica, Leucas cephalotes, Portulaca oleracea and Solanum nigrum) were investigated for their antioxidative potential using 2,2-dyphenyl-1-picrylhydrazyl (DPPH) scavenging, hydrogen peroxide (H2O2), ferric reducing antioxidant power (FRAP), and ferric thiocyanate (FTC) methods. Among the selected plant extracts C. tora displayed the highest DPPH radical scavenging activity with an IC50 value 9.898 μg/mL, whereas A. sessilis had the maximum H2O2 scavenging activity with an IC50 value 16.25 μg/mL—very close to that of ascorbic acid (16.26 μg/mL). C. tora showed the highest absorbance in the FRAP assay and the lowest lipid peroxidation in the FTC assay. A methanol extract of A. sessilis resulted in the greatest phenolic content (292.65 ± 0.42 mg gallic acid equivalent (GAE)/g) measured by the Folin–Ciocalteu reagent method, while the smallest content was recorded for B. alba (72.66 ± 0.46 GAE/g). The greatest flavonoid content was observed with extracts of P. oleracea (39.38 ± 0.57 mg quercetin equivalents (QE)/g) as measured by an aluminium chloride colorimetric method, while the least was recorded for I. aquatica (6.61 ± 0.42 QE/g). There was a strong correlation between antioxidant activity with total phenolic (DPPH, R2 = 0.75; H2O2, R2 = 0.71) and total flavonoid content (DPPH, R2 = 0.84; H2O2, R2 = 0.66). This study demonstrates that these wild edible leafy plants could be a potential source of natural antioxidants.

Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii

“…In this regard, excluding tyrosols, each polyphenol class analyzed was significantly correlated (p < 0.01) to the DPPH, FRAP, CUPRAC, and ABTS assays. In accordance with our results, polyphenols were reported as the main contributors to antioxidant properties in earlier studies [41,42]. On the other hand, only flavonoids (i.e., anthocyanins, flavanols, flavonols, and flavones) were found to be highly correlated to the enzymatic inhibition tested, with the most significant values (p < 0.01) recorded for flavanols (Supplementary Materials).…”

Prediction of Antioxidant Activity of Cherry Fruits from UAS Multispectral Imagery Using Machine Learning

“…The most interesting correlations were the To further explore interactions between the various variables affecting fruit quality, Pearson correlation was used, and Pearson's coefficients are listed in Table 4. Some interesting correlations was the negative correlation of DPPH with FCR, which was an expected finding, as it is known that polyphenols correlate with the antioxidant capacity of fruits and vegetables [67,68]. Leaf analysis data were not included in the PCA because represented a small fraction of the dataset, but some of them gave interesting correlations with DPPH and FCR.…”

Microscopic investigations and pharmacognostic techniques for the standardization ofCaralluma edulis(Edgew.) Benth. ex Hook.f.

“…Flavonoids are also present in plants and equally antioxidant because of the available -OH groups (Panche, Diwan, & Chandra, 2016). Hydroxyl groups are polar in nature, and they are extracted in a higher ratio with polar solvents as compared to nonpolar solvents (Aryal et al, 2019). The highest value of methanol extract is due to its polar nature.…”