Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa

Abstract

The present study describes the total phenolic content, concentrations of flavonoids and in vitro antioxidant and antimicrobial activity of methanol extracts from Seseli pallasii Besser, S. libanotis (L.) Koch ssp. libanotis and S. libanotis (L.) Koch ssp. intermedium (Rupr.) P. W. Ball, growing wild in Serbia. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 84.04 to 87.52 mg GA (gallic acid)/g. The concentrations of flavonoids in the extracts varied from 4.75 to 19.37 mg Qu (quercetin)/g. Antioxidant activity was analyzed using DPPH reagent. Antioxidant activity ranged from 0.46 to 4.63 IC50 (mg/ml) and from 1.98 to 2.19 mg VitC (vitamin C)/g when tested with the DPPH and ABTS reagents, respectively, using BHA and VitC as controls. The antimicrobial activity of the extracts was investigated using a micro-well dilution assay for the most common human gastrointestinal pathogenic bacterial strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Salmonella enteritidis ATCC 13076, Bacillus cereus ATCC 10876, Listeria monocytogenes ATCC15313, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231. This finding suggests that Seseli species may be considered as a natural source of antioxidants and antimicrobial agents.

Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (compositae) from Georgia

“…Based on the measured absorbance, the content of phenolics in the extracts was expressed in terms of the gallic acid (GA) equivalent (GAE) or mg of GA/g of extract. The flavonoid contents of the methanol, ethyl acetate and n-hexane extracts were determined by UV spectroscopy [33]. The sample contained 0.1 mL of the methanol solution of the extract at a concentration of 1 mg/mL, 0.41 mL 80% ethanol, and 0.01 mL and 0.1 mL of Al(NO 3 ) 3 x9H 2 O and CH 3 COOK each.…”

In vitrobiological activity of secondary metabolites fromSeseli rigidumWaldst. et Kit. (Apiaceae)

“…In the study of Matejić et al [16] methanolic extracts of tree Seseli taxa from Serbia were investigated. According to these authors, S. pallasii, S. libanostis ssp.…”

“…In case of S. annuum, germacrene, sabinene, ß-ocimene and limonene as main constituent was highly effective in inhibiting the growth of fungi [17], while according to Ozturk and Ercisli [19] trans-caryophyllene, ß-farnesene, eusarone and spathulenol were related to antimicrobial activity of S. libanotis essential oils. Several biological activities of the Seseli species were reported, including in vivo and in vitro anti-inflammatory, antinociceptive, antimicrobial and antioxidant activities [12,16,21].…”

Farinose flavonoids are associated with high freezing tolerance in fairy primrose (Primula malacoides) plants

“…In general, plants phenolics that include a broad range of compounds such as various phenylpropanoids, flavonoids, and anthocyanins can protect plant cells against damage caused by ROS during, for example, drought or UV light irradiation stresses (Winkel‐Shirley ; Buer et al ; Pollastri and Tattini ). Furthermore, flavonoids play important roles in deterring herbivorous insect and in antimicrobial resistance as phytoalexins (Harborne and Williams ; Matejic et al ). As introduced, the presence of various flavonoids was also closely correlated with levels of freezing tolerance in plants, pointing to yet another protective role of flavonoids in plants.…”