Total Phenolic Content and Free Radical Scavenging Activity of Representative Medicinal Plants of Thailand

Abstract

Objective: Plants are the vital source of pharmaceutically important compounds with less or no adverse side effects. The current study was conducted to catalog the commonly used indigenous and medicinal plants of Thailand based on their phenolic acid content and antioxidant activity. Methods:The herbs were collected from Chiang Mai province, Thailand. The plants were extracted with 70% ethanol. The total phenolic acid content and antioxidant activity were evaluated. Results:The ethanolic extract of plant samples was prepared. Among the tested plant samples, Phyllanthus emblica Linn. and Terminalia belerica Roxb. showed highest phenolic content (Gallic acid equivalent [GAE]; 764.81 mg GAE/g sample) and antioxidant activity (trolox equivalent antioxidant capacity [TEAC]; 394.20 mg/g sample), respectively. About 94-97% of inhibition of free radical was detected in 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay tested against the plant samples. The ethanolic extract of Anaxagorea luzonensis Gray., Terminalia sp., T. belerica Roxb, Terminalia chebula Retz., Albizia procera Benth., Harrisonia perforata Merr., and P. emblica Linn. exhibited 97.87, 96.08, 92.26, 86.74, 86.08, 84.47, and 83.13% of superoxide radical inhibition, respectively. Conclusion:The results suggested that T. belerica Roxb. possessed high TEAC ability and DPPH radical scavenging capacity and A. luzonensis Gray. exhibited high superoxide scavenging activity, when compared to that of the other tested samples. The additional detailed study is desirable to understand the complexity and distribution of bioactive compounds present in the commonly used plant species of Thailand.

Gymnema inodorum (Lour.) Decne. Extract Alleviates Oxidative Stress and Inflammatory Mediators Produced by RAW264.7 Macrophages

“…The literature survey reported that the leaves of G. inodorum had many phytochemical compounds such as phenolics, flavonoids, terpenoids, and glycoside [13,14]. Moreover, its antioxidant, antiadipogenesis, antidiabetic, and hypoglycemic effects were also reported [14][15][16]. Therefore, the phytochemical constituents presented in G. inodorum may contribute to its biological activity.…”

“…Therefore, the phytochemical constituents presented in G. inodorum may contribute to its biological activity. Numerous studies exhibited that the phytochemical compounds, especially flavonoids and phenolic content, had contributed to the redox-modulating properties of natural compounds, which had also been shown to modulate the inflammatory response virtually [15][16][17]. Along this line, it is possible that G. inodorum may exert a beneficial effect on alleviating intracellular ROS and inflammation.…”

The Total Polyphenolic Content and Antioxidant Properties of Various Honey and Sugars

“…The total phenolic content of the samples was determined by the modified Folin-Ciocalteu colorimetric method as described previously [26,27]. The absorbance was measured at 725 nm after 30 min incubation at room temperature (RT).…”

Formulation and Stability Assessment of Arabica and Civet Coffee Extracts Based Cosmetic Preparations

“…The TPC of the coffee extracts was determined by Folin-Ciocalteu colorimetric method as prescribed previously [10], and TPC was denoted as mg of gallic acid equivalent (GAE) per g of sample.…”