Total phenolic content and antioxidant activity of a medicinal plant endemic to the Algerian Sahara

Haci, Imad Abdelhamid El; Atik-Bekkara, Fawzia; Didi, Amal; Gherib, Mohammed; Didi, Mohamed Amine

doi:10.1007/s10298-012-0726-x

Cited by 16 publications

(11 citation statements)

References 21 publications

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“…Diverses plantes ont été rapportées présentant une activité antioxydante y compris Citrullus colocynthis, Limoniastrum feei, Pistacia lentiscus et bien d'autres [6][7][8].…”

Section: Mots Clés : Berberis Vulgaris -Extrait Hydrométhanolique -Alunclassified

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Évaluation de l’activité antioxydante de Berberis vulgaris L.

et al. 2014

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Les substances naturelles issues des végétaux présen-tent des intérêts multiples mis à profit dans la biotechnologie tant dans l'industrie alimentaire, cosmétique que pharmaceutique. Parmi ces composés, on retrouve une grande partie des métabolites secondaires qui se sont illustrés dans beaucoup de domaines et même en thérapie. Dans ce contexte, nous avons recherché l'activité antioxydante de Berberis vulgaris, une plante utilisée en Algérie et plus particulièrement dans la région de Tlemcen. Après préparation des extraits à partir des écorces de racines de Berberis vulgaris, les alcaloïdes totaux ont été analysés par chromatographie sur couche mince, révélant la présence de six taches. Aussi, une analyse quantitative des polyphénols et des flavonoïdes de l'extrait hydrométhanolique a été réalisée, montrant une teneur de 10,48 mg GAE/g en polyphénols totaux et 2,05 mg CEQ/g en flavonoïdes. L'activité antioxydante des extraits hydrométhanolique et alcaloïdes totaux a été évaluée par deux méthodes : la réduction du fer et le piégeage du radical libre DPPH. Les résultats obtenus ont montré une activité élevée de l'extrait hydrométhanolique par rapport aux alcaloïdes totaux, avec une CI 50 de 1,40 mg/ml. Mots clés : Berberis vulgaris -Extrait hydrométhanolique -Alcaloïdes totaux -Activité antioxydanteEvaluation of the Antioxidant Activity of Berberis vulgaris L.Abstract: Natural substances from plants have multiple interests exploited in biotechnology industry both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites that are illustrated in many areas and even in therapy. In this context, we investigated the antioxidant activity of Berberis vulgaris, a plant used in Algeria, and it is particularly used in the Tlemcen region. After preparation of extracts from the root bark of Berberis vulgaris, total alkaloids were analyzed by thin layer chromatography, indicating the presence of six tasks. A quantitative analysis of polyphenols and flavonoids in methanolic extract was carried out, showing a content of 10.48 mg GAE/g of total polyphenols and 2.05 mg CEQ/g of flavonoids. The antioxidant activity of hydromethanolic extracts under reflux and total alkaloids was evaluated using two methods: reducing power and DPPH free radical scavenging. The results showed a high activity of methanolic extract than the total alkaloids, with an IC 50 of 1.40 mg/ml.

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“…Diverses plantes ont été rapportées présentant une activité antioxydante y compris Citrullus colocynthis, Limoniastrum feei, Pistacia lentiscus et bien d'autres [6][7][8].…”

Section: Mots Clés : Berberis Vulgaris -Extrait Hydrométhanolique -Alunclassified

“…La matière végétale (20 g) est mise en contact avec 200 ml du mélange méthanol/eau (70/30) (v/v) et est portée sous reflux pendant trois heures [8]. L'extrait obtenu est filtré puis concentré à sec (T = 60°C) par un rotavapeur (Heidolph).…”

Section: Préparation De L'extrait Hydrométhanoliqueunclassified

Évaluation de l’activité antioxydante de Berberis vulgaris L.

et al. 2014

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“…Then, a volume of 100 μL Folin-Ciocalteu's reagent was added. The mixture was incubated for 30 min and absorbance was read at 700 nm to determine the TPL as mg gallic acid per g dry weight (mg GAE/g) [20].…”

Section: Determination Of Total Phenolic Level (Tpl)mentioning

confidence: 99%

Phytochemicals Levels and Antioxidant Capacities of Figs Flowers Fruits

Mohammed

Merah

2020

Nepal J Biotechnol

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Since antiquity, phenolic compounds produced by plants were known as free radical scavengers and as powerful antioxidants. Huge interest has been made by researchers to the traditional uses of medicinal plants against illnesses related to oxidative stress. This study measures the correlation that can be existed between the antioxidant capacity and phytochemical s levels of four varieties of Ficus carica fruits, figs flowers or "Bakor" as called locally in Algeria. Therefore, extracts were assessed for determining their antioxidative potentials using both test of total antioxidant capacity and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging test followed by quantitative phytochemical analysis to estimate the total flavonoid level (TFL), the total phenolic level (TPL), the total anthocyanin level (TAL) and the condensed tannins level of plants methanolic extracts. A positive correlation was observed between phenolics content and the antioxidant capacity of figs flowers methanol extracts. The methanolic extract of Bechar (MeOH Var.2) chelated 87,9± 1,23 % of the DPPH free radical with IC50 value equal to 0,185 mg/g DW. A high antioxidant ability of almost all extracts is, probably, related to the appreciable rates of flavonoids, phenolics and tannins showed by those fig extracts. The highest value of phenolics level was detected among the variety 1 methanolic extract of Bechar (MeOH Var.1) of 10,4 mg GAE/g DW.

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“…Indeed, the extraction of phenolic compounds from their natural matrix is complicated by their diversity and their susceptibility to oxidation and hydrolysis (Naczk and Shahidi 2004). Similarly, several factors can influence quantifications such as variety, environmental conditions, the mode of conservation substrates extraction, the degree of fruit ripening and genetic factors (Ebrahimzadeh et al 2008;El-Haci et al 2012).…”

Section: Total Phenolic Flavonoid Of Samplementioning

confidence: 99%

In-vitro antioxidant and antimicrobial activities of some varieties citrus grown in Algeria

Haraoui

Allem

Chaouche

et al. 2019

ADV TRADIT MED (ADTM)

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Polyphenols are scientifically researched molecules because of their multiple biological activities. This study aims to compare the phenolic content and estimate the antioxidant and anti-microbial activities of leaf extracts and juice of ten varieties Citrus fruits grown in Algeria. Antioxidant properties were evaluated by DPPH radical scavenging activity, β-carotene-linoleic acid system and ferric reducing antioxidant capacity. Antimicrobial activity was carried out using agar well diffusion method. The results showed the highest levels of phenolic compounds in juice and leaves of different part and varieties of plant (*P < 0.05). Among all the varieties, C. maxima and C. aurantium presents the highest activity for the three methods cited previously (94.44-91.66% and 95.41-83.84%). On the other hand, antibacterial activity present on the plates was indicated by an inhibition zone surrounding the well containing the juice. The C. Limon and C. aurantium varieties exhibited the highest antimicrobial activity, with inhibition zone ranging from (27.66 ± 0.58, 24.66 ± 1.53 mm) followed by grapefruit and mandarin with areas between (23.00 ± 1.00, 17.66 ± 0.58 mm) on the isolates used. In this study, whatever the parts of citrus (juice and leaves) have been found to contain higher amounts of phenolics and their efficiency as potent antioxidants and antimicrobial properties was confirmed.

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Total phenolic content and antioxidant activity of a medicinal plant endemic to the Algerian Sahara

Cited by 16 publications

References 21 publications

Évaluation de l’activité antioxydante de Berberis vulgaris L.

Évaluation de l’activité antioxydante de Berberis vulgaris L.

Phytochemicals Levels and Antioxidant Capacities of Figs Flowers Fruits

In-vitro antioxidant and antimicrobial activities of some varieties citrus grown in Algeria

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