Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail millet with differential salt tolerance

Sreenivasulu, Nese; Sunkar, Ramanjulu; Ramachandra-Kini, K; Prakash, H. S.; Shekar-Shetty, H; Savithri, H.S.; Sudhakar, Chinta

doi:10.1016/s0168-9452(98)00204-0

Cited by 104 publications

(67 citation statements)

References 16 publications

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“…Previous studies with Triticum aestivum L., Oriza sativa L., Zea mays L. evidenced that salt-tolerant genotypes had a more efficient antioxidative system (Sairam et al, 2002;Demiral and Turkan, 2005;De Azevedo Neto et al, 2006). According to Sreenivasulu et al (1999), the increased enzyme activity of APX may play a key role in the tolerance to oxidative stress. The APX and CAT activity in our study remained unchanged under moderate salt stress.…”

Section: Morphologicalmentioning

confidence: 99%

Morpho-physiological adaptation of Jatropha curcas L. to salinity stress

Cavalcante¹,

Santos²,

Filho³

et al. 2018

Aust J Crop Sci

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The aim of the current study is to assess the growth response, biochemical changes, photosynthetic pigments content and gas exchanges of physic nut (Jatropha curcas) grown under natural saline conditions. The experiment was arranged in a completely randomized design based on the following soil electrical conductivities: 0.29 dS.m -1 (control), 1.76 dS.m -1 (moderate salt concentration), 2.61 dS.m -1 (high salt concentration), 3.79 dS.m -1 (very high salt concentration). Physic nut plants were kept under saline conditions for 19 days in greenhouse. Plant growth analyses were performed on a weekly basis. Plant biomass allocation was quantified at the end of the experiment. Leaf gas exchange, stomatal conductance, Fv/Fm and quantum yield were quantified 102 days after planting. Photosynthetic pigments, amino acids, proline and carbohydrates in fresh leaf tissue were also quantified. The leaf antioxidant enzymes catalase (CAT) and ascorbate peroxidase (APX) were quantified. Although there was no alteration in biomass allocation, the initial growth of J. curcas was gradually reduced by increasing salt concentration, which was observed through reduced plant height, stem diameter, and total number of leaves. Soil electrical conductivity 3.79 dS.m -1 was lethal to seedlings. Seedlings exposed to salt stress had their photosynthesis, stomatal conductance, transpiration and effective photochemical efficiency reduced, and their catalase and ascorbate peroxidase enzyme activity increased. Amino acids, proline and carbohydrate concentrations increased due to salt stress, whereas there was decrease in the chlorophyll content. J. curcas is sensitive to soil salinity at electrical conductivity levels higher than 1.76 dS.m -1 . To some extent, salinity effects can be relieved by osmolyte accumulation and by greater antioxidant activity; however, these factors were not sufficient to keep plant growth within normal rates.

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Section: Morphologicalmentioning

confidence: 99%

Morpho-physiological adaptation of Jatropha curcas L. to salinity stress

Cavalcante¹,

Santos²,

Filho³

et al. 2018

Aust J Crop Sci

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“…The production of reactive oxygen species is a common phenomenon in plants under drought stress. These reactive oxygen species (ROS) generations led to lipid peroxidation (Sreeni-Nivasuhu et al 1999, Chen et al 2000, protein degradation (Jiang and Zhang 2001) and nucleic acid damages (Hagar et al 1996). To alleviate adverse effects of reactive oxygen species, plants have evolved an antioxidant defense system that include enzymes like superoxide dismutase, peroxidase and catalase etc.…”

mentioning

confidence: 99%

Role of abscisic acid and drought stress on the activities of antioxidant enzymes in wheat

Bano¹,

Ullah²,

Nosheen³

2012

Plant Soil Environ.

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The effect of drought stress and abscisic acid (ABA) applied at tillering stage (55 days after sowing) was compared in 2 wheat cultivars differing in drought tolerance. The activities of superoxide dismutase (SOD) and peroxidase (POD) and contents of endogenous ABA in plants were measured at 3 days of drought stress in cv. Chakwal-97 (drought tolerant) and cv. Punjab-96 (drought susceptible). ABA was applied at 10 -6 mol/L as presowing seed treatment for 18 h. Drought tolerant cultivar has a more efficient mechanism to scavenge reactive oxygen species as shown by a significant increase in the activity of antioxidant enzyme SOD. Under drought stress, ABA significantly increased the activities of SOD and POD, showing a significant decline on rewatering. The relative water content was significantly increased by ABA priming under drought stress in both wheat cultivars. The sensitive cultivar exhibiting lower endogenous ABA content was more responsive to ABA priming. On rewatering, the magnitude of recovery from drought stress was greater in tolerant cultivar. ABA was highly effective in improving grain weight of tolerant cultivar under drought stress.

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“…Após a corrida eletroforética, os géis foram revelados e corados em soluções apropriadas (Alfenas, 1998), para os seguintes sistemas isoenzimáticos: álcool desidrogenase (ADH), esterase (EST), malato desidrogenase (MDH), peroxidase (PO), glutamato oxaloacetato transaminase (GOT) e fosfatase ácida (ACP). Os sistemas isoenzimáticos foram selecionados em função de apresentarem atividades em trabalhos realizados com bananeira (Bhat et al, 1992;Gomes et al, 2002;Ulisses et al, 2002). O polimorfismo foi analisado segundo a presença ou ausência de bandas e também segundo a intensidade de bandas.…”

Section: Methodsunclassified

“…Esses dois genótipos, SN o /2 e M53, formaram um grupo à parte, conforme se discute mais adiante, considerando os dendrogramas construídos. O incremento da atividade de peroxidases é fundamental no controle da ação de espécies de oxigênio reativas responsáveis por danos oxidativos freqüentes em plantas submetidas ao estresse salino (Sreenivasulu et al, 1999).…”

Section: Genótipos ------------Limbo Foliar------------unclassified

“…Além dos distúrbios fisiológicos, ocorrem variações nos padões dos sistemas isoenzimáticos, sobretudo das peroxidases, em plantas submetidas ao estresse salino. A expressão de bandas específicas de peroxidase em genótipos tolerantes à salinidade foi constatada por Sreenivasulu et al (1999) em Setaria italica, e por Ulisses et al (2002) em somaclones de bananeira.…”

Section: Introductionunclassified

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Diplóides (AA) de bananeira submetidos ao estresse salino

Gomes

Willadino

Martins

et al. 2004

Pesq. agropec. bras.

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Resumo -No Nordeste do Brasil a salinização dos solos é um dos fatores limitantes na produção de bananeira. Estudos quanto à tolerância à salinidade em diplóides de bananeira são importantes para programas de melhoramento genético. Esse trabalho objetivou avaliar os efeitos da salinidade utilizando variáveis químicas e de crescimento, e quantificar, mediante padrões isoenzimáticos, a diversidade genética entre seis genótipos diplóides (AA), associando-os à tolerância à salinidade. As plantas foram tratadas durante 21 dias com 0, 50 e 100 mM de NaCl, num delineamento experimental inteiramente casualizado. Os diplóides Lidi e Calcuttá apresentaram maiores reduções na área foliar e fortes sintomas de toxidez associados aos maiores acúmulos de Na + e Cl -no limbo. Os genótipos Borneo e SN o /2 apresentaram discretos sintomas de toxidez e, como o genótipo M-53, demonstraram habilidade de evitar a translocação excessiva de Na + e Cl -para as folhas preservando o aparelho fotossintético. Nos diplóides SN o /2 e M-53 foi detectada uma banda específica (Po-6) do sistema peroxidase, sob condições de estresse salino. Associando as características isoenzimáticas com as de crescimento, sintomatologia, análise mineral e grau de similaridade genética entre os genótipos, os dendrogramas construídos separam os genótipos mais tolerantes (SN o /2 e M-53) dos mais sensíveis (Lidi e Calcuttá).Termos para indexação: Musa, salinidade, isoenzimas. Banana diploids (AA) submitted to salt stressAbstract -In the Northeast Region of Brazil, salinization in soils is one of the limiting factors for banana production. Studies regarding tolerance and salt sensitivity in banana diploids are important for genetic breeding programs. This work aimed to evaluate salt stress effects measuring growth and chemicals parameters and to quantify the genetic diversity among six diploids (AA) genotypes using isozymic standards relating to salt tolerance. The plants were treated during 21 days under 0, 50 and 100 mM of NaCl in a completely randomized design. The diploids Lidi and Calcuttá presented the greatest leaf area reduction and severe toxicity associated to greater Na + and Cl -accumulation in leaf blades. The genotypes Borneo and SN o /2 presented discrete symptoms of toxicity and like M-53 genotype, demonstrated the capacity of avoiding excessive translocation of Na + and Cl -to the leaves preserving the photosynthetic apparatus. Only the diploid SN o /2 and M-53 showed a specific peroxidase band (Po-6), under salt stress. The relationship between the isoenzymatic and the growth and mineral analysis of vegetal tissue created dendrograms separating the most tolerant genotypes (SN o /2 and M-53) from the most sensitive ones (Lidi and Calcuttá).

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Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail millet with differential salt tolerance

Cited by 104 publications

References 16 publications

Morpho-physiological adaptation of Jatropha curcas L. to salinity stress

Morpho-physiological adaptation of Jatropha curcas L. to salinity stress

Role of abscisic acid and drought stress on the activities of antioxidant enzymes in wheat

Diplóides (AA) de bananeira submetidos ao estresse salino

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