Total on-line analysis of a target protein from plasma by immunoextraction, digestion and liquid chromatography–mass spectrometry

Cingöz, A.; Hugon-Chapuis, F.; Pichon, Valérie

doi:10.1016/j.jchromb.2009.07.032

Cited by 33 publications

(25 citation statements)

References 42 publications

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“…As an improved method to achieve both rapid speed and automated tryptic digestion, the use of a trypsinimmobilized enzyme reactor (IMER) as a pretreatment column, placed in front of an LC system, has been reported. 24,25 Not only IMERs, 26,27 but on-line tryptic digestion-LC system combined with the IMER, dedicated buffers, and columnswitching systems are also commercially available now. 28 As a pretreatment column in IMER, Monospin Trypsin column which is a trypsin-immobilized monolithic silica spin column, has been used.…”

Section: ·2 Efficiency Technology Speed Up and Automation Of Tryptmentioning

confidence: 99%

Current Mass Spectrometric Tools for the Bioanalyses of Therapeutic Monoclonal Antibodies and Antibody-Drug Conjugates

et al. 2018

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The increase in the use of therapeutic monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) has made the detailed bioanalysis of these drugs essential not only for planning optimal therapeutic programs for clinical practice, but also for evaluating the biological equivalencies in the development of other biosimilars. The ligand binding assays that are widely in use now are being replaced rapidly by the highly accurate, sensitive, and selective analytical method using a mass spectrometer. This review will discuss the progress in and challenges observed during the development of a mass spectrometry-based bioanalytical method for therapeutic mAbs and ADCs.

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Section: ·2 Efficiency Technology Speed Up and Automation Of Tryptmentioning

confidence: 99%

Current Mass Spectrometric Tools for the Bioanalyses of Therapeutic Monoclonal Antibodies and Antibody-Drug Conjugates

et al. 2018

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“…An efficient and alternative method is to immobilize trypsin on a solid support to create flow-through reactors that can speed up the process. In addition to flow-through reactors, it is also possible for flow of protein solution to be paused in a reactor to allow for digestion with the flow then resumed [158,159]. Autolysis is minimized when the enzymes are immobilized in enzyme reactors [142].…”

Section: Monolithic Reactor Columns In Digestion Of Glycoproteins Andmentioning

confidence: 99%

Development of Monolithic Column Materials for the Separation and Analysis of Glycans

Alla

Stine

2015

Chromatography

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Monolithic column materials offer great advantages as chromatographic media in bioseparations and as solid-supports in biocatalysis. These single-piece porous materials have an interconnected ligament structure that limits the void volume inside the column, thus increasing the efficiency without sacrificing the permeability. The preparation of monolithic materials is easy, reproducible and has available a wide range of chemistries to utilize. Complex, heterogeneous and isobaric glycan structures require preparation methods that may include glycan release, separation and enrichment prior to a comprehensive and site-specific glycosylation analysis. Monolithic column materials aid that demand, as shown by the results reported by the research works presented in this review. These works include selective capture of glycans and glycoproteins via their interactions with lectins, boronic acids, hydrophobic, and hydrophilic/polar functional groups on monolith surfaces. It also includes immobilization of enzymes trypsin and PNGase F on monoliths to digest and deglycosylate glycoproteins and glycopeptides, respectively. The use of monolithic capillary columns for glycan separations through nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC) and coupling these columns to MS instruments to create multidimensional systems show the potential in the development of miniaturized, high-throughput and automated systems of glycan separation and analysis.

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“…To solve these problems, much attention has been paid to the development of immobilized enzymatic reactors (IMERs) [72][73][74][75][76]. In recent years, numerous immobilized trypsin reactors have been developed, and coupled with HPLC-MS/MS or CE-MS/MS to achieve high throughput analysis of proteomes [77][78][79]. Ma et al [80] developed an organic-inorganic hybrid silica monolith based immobilized trypsin reactor, and integrated it with HPLC/MS/MS.…”

Section: Integrated Platformsmentioning

confidence: 99%

Recent advances on multidimensional liquid chromatography–mass spectrometry for proteomics: From qualitative to quantitative analysis—A review

Yuan

Zhang

et al. 2012

Analytica Chimica Acta

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Total on-line analysis of a target protein from plasma by immunoextraction, digestion and liquid chromatography–mass spectrometry

Cited by 33 publications

References 42 publications

Current Mass Spectrometric Tools for the Bioanalyses of Therapeutic Monoclonal Antibodies and Antibody-Drug Conjugates

Current Mass Spectrometric Tools for the Bioanalyses of Therapeutic Monoclonal Antibodies and Antibody-Drug Conjugates

Development of Monolithic Column Materials for the Separation and Analysis of Glycans

Recent advances on multidimensional liquid chromatography–mass spectrometry for proteomics: From qualitative to quantitative analysis—A review

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