Total internal reflection fluorescence (TIRF) as a quantitative probe of protein adsorption

Rockhold, S.; Quinn, Roswell; Wagenen, R.A. Van; Andrade, Joseph D.; Reichert, Manuela

doi:10.1016/s0022-0728(83)80209-5

Cited by 41 publications

(9 citation statements)

References 8 publications

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“…Light propagating in the fiber at angles of incidence greater than this angle, termed the critical angle, are guided by the fiber. The electric field amplitude of the guided wave decays exponentially in the lower index medium with the exponent dependent on the refractive indices of the fiber and medium, the angle of incidence at the interface, as well as the wavelength of light (Rockhold, 1983). Evanescent wave refers to the electric field in the rarer medium and the penetration depth defines the distance over which its amplitude decreases to 1/e of the value at the interface.…”

Section: Waveguidesmentioning

confidence: 99%

“…UV-Vis spectroscopy has been adapted to remote sensing of various processes, for example, biological functions, (Andrade, 1985; Dahne, 1984;Newby, 1984Newby, , 1986Peterson, 1984;Rockhold, 1983) chemical mixing, (Fitch, 1985; Wang, 1989) addition of colorants to polymers, (Fitch, 1985) as well as to cure monitoring. Levy, 1983A, 1983B, 1984Sung, 1985Sung, , 1986Wang, 1985.…”

Section: Absorption and Fluorescence Of Reactive Speciesmentioning

confidence: 99%

“…The other spectroscopy types mentioned above have potential for remote sensing through the use of optical fiber waveguides (Andrade, 1985;Boisde, 1984;Dahne, 1984;Giallorenzi, 1978;Hirschfield, 1983;Lew, 1984;Maugh, 1982;Munkholm, 1986;Newby, 1984Newby, , 1986Peterson, 1984;Rockhold, 1983;Schirmer, 1988;Schwab, 1984;Tromberg, 1984;Weyer, 1987). The combination of optical fibers and sensing elements are referred to as fiber optic sensors (FOS).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the state-of-the-art for process monitoring sensors for polymer composites

Hunston¹,

McDonough²,

Fanconi³

et al. 1991

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A variety of techniques have been applied to monitoring the changes that occur in the resin during composite processing. This report identifies and analyzes those techniques that have the most promise for on-line application, namely: ultrasonic, dielectric, spectroscopic, and optical techniques. All these methods have advantages and disadvantages, and the best technique for a given application will depend strongly on the materials and processes involved. This report discusses these on-line monitoring techniques and compares them in the areas of measurement speed, sampling volume, sensitivity to different resins, effects of fiber type, resistance to the manufacturing environment, interpretation of the data, adaptability for other uses, and temperature capabilities. The most important comparison, however, concerns the research and development work that is needed to achieve the full potential of each technique in applications on the factory floor. Dielectric and ultrasonic methods have an advantage in this connection because commercial equipment designed for process monitoring is already available. The various spectroscopic and optical methods are generally in an earlier stage of development. The great potential inherent in these latter methods, however, makes it highly desirable to actively pursue their developments as well. Although each of the monitoring techniques has its own research and development needs, the most important technical and scientific issues are common to all. From a technological standpoint, the major short term need is to build more rugged and reliable equipment that can withstand the harsh manufacturing environment. Even commercially available systems would benefit from such improvements, particularly in the sensors, electrical connections, and/or electronics, so process monitoring can be used with more confidence on the factory floor. It is also important to develop better systems for operating in electrically noisy environments and with "impure" industrial-grade resins. Solutions to these technical problems are critical to progress in this field, and considerable effort will be required to solve them, but the approach is straightforward. interpretation, and implementation of sensors have not received sufficient attention. Consequently, research and development on sensors is badly needed. Moreover, it could leverage the work performed in projects addressing other aspects of the process control area and thereby play a vital role. The scientific questions outlined above are particularly appropriate since the results will have maximum impact on the development of on-line monitoring techniques. contract programs on this topic. There are major thrusts by the Air Force, NASA, and the National Institute of Standards and Technology. NSF, DARPA, the Office of Naval Research, the Army Research Office, and the Army's Materials Technology Laboratory also have at least some projects on composite process control. Through contracts and subcontracts, many universities are involved in these programs as well. ...

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Section: Waveguidesmentioning

confidence: 99%

Section: Absorption and Fluorescence Of Reactive Speciesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of the state-of-the-art for process monitoring sensors for polymer composites

Hunston¹,

McDonough²,

Fanconi³

et al. 1991

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“…Total internal reflection fluorescence (TIRF) microscopy, also known as evanescent wave microscopy, has been widely used in bioimaging [1,2], chemical sensing [3,4], and biophysical applications [5,6]. This method provides low-noise, high-quality images of specimen containing fluorophores, such as quantum dots or fluorescent molecules.…”

Section: Introductionmentioning

confidence: 99%

Design of plasmonic nano-antenna for total internal reflection fluorescence microscopy

et al. 2013

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We propose a gold modified bow-tie plasmonic nano-antenna, which can be suitably used in combination with total internal reflection fluorescence microscopy. The plasmonic nano-antenna, supporting well-separated multiple resonances, not only concentrates the total internal reflection evanescent field at the deep subwavelength scale, but also enhances fluorescence emission by the Purcell effect. Finite-difference time-domain computations show that the enhancement of the excitation light strongly correlates with the far-field radiation pattern radiated from the antenna. Depending on the antenna geometry, the resonant modes are widely tuned and their wavelengths can be easily matched to the diverse emission or excitation wavelengths of fluorophores.

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“…Waveguide interferometric biosensing exploits the differential change in the phase velocity of the guided modes of a waveguide caused by biological particles bound to the surface. While other optical biosensing techniques, e.g., total internal reflection fluorescence [5], [6], tag particles and sense the resulting fluorescence, waveguide interferometry detects any particle bound to the surface though a change in the index of refraction. Thus, the selectivity of the surface binding chemistry determines how well the biosensor can distinguish small numbers of the desired particle type.…”

mentioning

confidence: 99%

Surface desensitization of polarimetric waveguide interferometers

worth

Goldberg

Ruane

et al. 2001

IEEE J. Select. Topics Quantum Electron.

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Nonspecific surface binding of small protein molecules presents a major obstacle to surface biosensing techniques attempting to detect very low concentrations (1 pg/mm 2) of large biological objects such as cells and bacteria. A new method for selective desensitization of a polarimetric waveguide interferometer eliminates the background noise from nonspecific surface binding. We demonstrate the ability to tune the phase sensitivity of a waveguide interferometer as a function of the distance of the biological or chemical analyte from the waveguide surface. This makes possible a sensor that has zero sensitivity at a particular distance where nonspecific surface binding occurs without significantly reducing the sensitivity to target larger biological species.

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Total internal reflection fluorescence (TIRF) as a quantitative probe of protein adsorption

Cited by 41 publications

References 8 publications

Assessment of the state-of-the-art for process monitoring sensors for polymer composites

Assessment of the state-of-the-art for process monitoring sensors for polymer composites

Design of plasmonic nano-antenna for total internal reflection fluorescence microscopy

Surface desensitization of polarimetric waveguide interferometers

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