Total Hydroxycinnamic Acids Assay: Prevalidation and Application on Lamiaceae Species

Štefan, Maja Bival; Rodríguez, Jadranka Vuković; Blažeković, Biljana; Kindl, Marija; Vladimir‐Knežević, Sanda

doi:10.1007/s12161-013-9630-8

Cited by 29 publications

(21 citation statements)

References 27 publications

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“…The majority of our results are in agreement with the results reported in previous studies, which demonstrated variation in the RS content detected in Lamiaceae extracts in the range 3.48–90.52 mg·g −1 d.m. ( 19 , 25 – 28 ). The only exception were the results concerning RO extract.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical screening and biological activity of Lamiaceae family plant extracts

et al. 2017

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The current study aimed to investigate ethanolic extracts from the following medicinal plant species cultivated in western Romania: Melissa officinalis L., Rosmarinus officinalis L. (RO) and Salvia officinalis L. (SO). Antioxidant activity, total phenolics content and a profile of the main hydroxycinnamic acids (HCAs), including caffeic, ferulic, coumaric and rosmarinic acids, was determined for each plant extract. The in vitro antimicrobial activity against four bacterial strains (Escherichia coli, Listeria-, Pseudomonas aeruginosa and Staphylococcus aureus), and the effect on cell viability in two melanoma cell lines (B164A5 murine melanoma and A375 human melanoma) was also assessed. The results indicated that total phenolics content was 73.76–274.73 mg GAE·g−1 and the antioxidant activity was 2.32–2.87 mM Fe2+·100 g−1. There was found a strong positive correlation (R=0.9691) between total phenolics content and the antioxidant activity in the investigated samples. Regarding the HCA profile obtained by high performance liquid chromatography, the results demonstrated that rosmarinic acid represents the main identified compound. The ethanolic extracts of RO and SO exhibited antibacterial activity against Gram positive and Gram negative bacteria. RO was the most effective in terms of decreasing the cell viability of murine and human melanoma cell lines, while the HCAs did not exhibit any effect on cell viability. These findings suggest that plant extracts from the Lamiaceae family may used in the clinic as natural antibacterial agents.

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Section: Resultsmentioning

confidence: 99%

Phytochemical screening and biological activity of Lamiaceae family plant extracts

et al. 2017

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“…Total proanthocyanidins were quantified as catechin equivalents using a catechin standard curve. Determination of total hydroxycinnamic acid (THA) was performed by colorimetric methods using the chromogenic system of HCl-NaNO 2 –Na 2 MoO 4 –NaOH with chlorogenic acid standard ( Štefan et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Cultivar Variation in Hormonal Balance Is a Significant Determinant of Disease Susceptibility to Xanthomonas campestris pv. campestris in Brassica napus

et al. 2017

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This study aimed to directly elucidate cultivar variation in disease susceptibility and disease responses in relation to hormonal status in the interaction of Brassica napus cultivars and Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease. Fully expanded leaves of six B. napus cultivars (cvs. Capitol, Youngsan, Saturnin, Colosse, Tamra, and Mosa) were inoculated with Xcc. At 14 days post-inoculation with Xcc, cultivar variation in susceptibility or resistance was interpreted with defense responses as estimated by redox status, defensive metabolites, and expression of phenylpropanoid synthesis-related genes in relation to endogenous hormonal status. Disease susceptibility of six cultivars was distinguished by necrotic lesions in the Xcc-inoculated leaves and characterized concurrently based on the higher increase in reactive oxygen species and lipid peroxidation. Among these cultivars, as the susceptibility was higher, the ratios of abscisic acid (ABA)/jasmonic acid (JA) and salicylic acid (SA)/JA tended to increase with enhanced expression of SA signaling regulatory gene NPR1 and transcriptional factor TGA1 and antagonistic suppression of JA-regulated gene PDF 1.2. In the resistant cultivar (cv. Capitol), accumulation of defensive metabolites with enhanced expression of genes involved in flavonoids (chalcone synthase), proanthocyanidins (anthocyanidin reductase), and hydroxycinnamic acids (ferulate-5-hydroxylase) biosynthesis and higher redox status were observed, whereas the opposite results were obtained for susceptible cultivars (cvs. Mosa and Tamra). These results clearly indicate that cultivar variation in susceptibility to infection by Xcc was determined by enhanced alteration of the SA/JA ratio, as a negative regulator of redox status and phenylpropanoid synthesis in the Brasica napus–Xcc pathosystem.

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“…Determination of total hydroxycinnamic acids was performed using method previously described by Štefan et al [ 17 ]. The content of total hydroxycinnamic acids, expressed as rosmarinic acid, was calculated from the expression: (%) = A × 2.5/ m , where A is the absorbance of the test solution at 505 nm and m is the mass of the sample, in grams.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Anticholinesterase Potential of SixThymusSpecies

Kindl

Blažeković

Bučar

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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The present study aimed to evaluate antioxidant and acetylcholinesterase (AChE) inhibitory activities of the ethanolic extracts of six selected Thymus species growing in Croatia (T. longicaulis, T. praecox subsp. polytrichus, T. pulegioides, T. serpyllum subsp. serpyllum, T. striatus, and T. vulgaris). Antioxidant effectiveness was assessed using six different assays, in comparison with rosmarinic acid, luteolin, and reference antioxidants. All tested Thymus extracts possessed DPPH (IC50 = 3–6 μg/mL) and nitric oxide (IC50 = 70–177 μg/mL) free radical scavenging activities, strong reducing properties (IC50 = 11–15 μg/mL), ferrous ion chelating activity (IC50 = 126–389 μg/mL), ability to inhibit lipid peroxidation (IC50 = 34–80 μg/mL), and high total antioxidant capacities (238–294 mg AAE/g). AChE inhibitory activity was examined using Ellman's colorimetric method and all tested extracts showed anti-AChE activity in a dose dependent manner. The values of 10–28%, 23–39%, and 64–86% were obtained for tested concentrations of 0.25, 0.5, and 1 mg/mL, respectively. Additionally, the contents of total hydroxycinnamic derivatives, flavonoids, and tannins in dried plant samples were determined spectrophotometrically. Our results highlighted Thymus species as a rich source of natural antioxidants and AChE inhibitors that could be useful in preventing and treating Alzheimer's disease and other neurodegenerative disorders.

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Total Hydroxycinnamic Acids Assay: Prevalidation and Application on Lamiaceae Species

Cited by 29 publications

References 27 publications

Phytochemical screening and biological activity of Lamiaceae family plant extracts

Phytochemical screening and biological activity of Lamiaceae family plant extracts

Cultivar Variation in Hormonal Balance Is a Significant Determinant of Disease Susceptibility to Xanthomonas campestris pv. campestris in Brassica napus

Antioxidant and Anticholinesterase Potential of SixThymusSpecies

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