Total homocysteine levels in plasma: high-performance liquid chromatographic determination with electrochemical detection and glassy carbon electrode

D'Eramo, José L.; Finkelstein, AbrahamE.; Boccazzi, FernandoO.; Fridman, Osvaldo

doi:10.1016/s0378-4347(98)00421-6

Cited by 47 publications

(25 citation statements)

References 30 publications

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“…Homocysteine was assayed by using high pressure liquid chromatography apparatus equipped with electrochemical detector as described by J.L.D'Eramo et al (13). Here sodium borohydride (NaBH 4 ) was used as a reductant and 0.1M monochloroacetic acid and 3.6mM sodium octylsulfate adjusted to pH 3.2 was used as the mobile phase.…”

Section: Methodsmentioning

confidence: 99%

Risk prediction—Homocysteine in Coronary Heart Disease

Govindaraju²,

Manjunath

2007

Indian J Clin Biochem

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Section: Methodsmentioning

confidence: 99%

Risk prediction—Homocysteine in Coronary Heart Disease

Govindaraju²,

Manjunath

2007

Indian J Clin Biochem

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“…[7][8][9]36 Consequently, reports that focus on electrochemical detection in human plasma are usually coupled with a separation technique, such as HPLC. [1][2]4,8,15,17,[20][21][22][23] In our experiments, human plasma was diluted to 25% with 0.15 M PBS (pH 7.2), and an optimal catechol concentration of 1.0 mM was used, because higher concentrations resulted in coagulation. Lastly, all three electrodes on the disposable screen-printed electrode, itself, were fully used due to the small quantity of available human plasma.…”

Section: Detection In Human Plasmamentioning

confidence: 99%

“…The shift of the unknown electroactive species could be attributed to the silver quasi-reference electrode built into the screen-printed electrode. Since human plasma alone contains contents of homocysteine and glutathione, 1,5,8,16,18,20,[37][38] we can expect to see an adduct peak signal associated with the thiol-catechol reaction. To definitively determine the peak observed at ca.…”

Section: Detection In Human Plasmamentioning

confidence: 99%

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Selective Thiol Detection in Authentic Biological Samples with the Use of Screen-printed Electrodes

Lee

Compton

2015

ANAL. SCI.

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A selective voltammetric determination of homocysteine and glutathione was applied to cell tissue culture media and human plasma via a single two-step method. The two-step method relies on the 1,4-Michael addition reaction between electro-oxidized catechol and the target thiol. Furthermore, the procedure relies on the differing reaction kinetics of the ortho-quinone with various thiol species giving different responses as a function of the scan rate. At faster scan rates homocysteine is only detected, while at slower scan rates the adduct signal reflects both homocysteine and glutathione. As a result, the quantification of both homocysteine and glutathione can be determined with a combination of both sets of data. The previous proof-of-concept (P. T. Lee, D. Lowinsohn, and R. G. Compton, Sensors, 2014, 14, 10395), is applied to the quantification of thiols in both tissue culture media and human plasma alone. Analytical parameters were determined for both homocysteine and glutathione in the respective media and the linear range. The sensitivities in tissue culture media are ca. 3 nA μM -1 and ca. 1 nA μM -1 and the limits of detections are ca. 2 μM and ca. 1 μM for homocysteine and glutathione, respectively. In human plasma, the sensitivities were determined to be 94 and 39 nA μM -1 , and the limit of detections are ca. 0.8 μM and ca. 0.8 μM for homocysteine and glutathione, respectively.

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“…Over the past decade, ion chromatography (IC), together with pulsed electrochemical detection (PED), has been accepted [21][22][23][24] as highly sensitive and selective methods to detect thiols, disulfides. In addition, both reduced and oxidized thiol moieties can be easily detected without the need of derivatization [12].…”

Section: Introductionmentioning

confidence: 99%

Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection

Wang¹,

Lin²

2017

Mod Chem Appl

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A novel method for the determination of isopropyl-β-D-thiogalactopyranoside (IPTG), a sulfur-containing compound, a pharmaceutical additive in commercial recombinant human growth hormone (rhGH), has been developed using pulsed electrochemical detection (PED), following ion Chromatographic separation. A Dionex-500 ion chromatograph coupled with an electrochemical detector was employed, equipped with a gold working electrode and an Acclaim 300 analytical C18 column, with a mobile phase consisting of sodium acetate (NaOAc) buffer (pH 5.45, 0.01 mol/L) and acetonitrile (ACN)(90/10, v/v). Upon optimization, IPTG was found to have a limit of detection of 1 ng/mL (0.1 μmol) with 25 uL injection volume. This method was successfully applied to the determination of IPTG in rhGH samples with the characteristics of simplicity, high sensitivity and good repeatability.

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Total homocysteine levels in plasma: high-performance liquid chromatographic determination with electrochemical detection and glassy carbon electrode

Cited by 47 publications

References 30 publications

Risk prediction—Homocysteine in Coronary Heart Disease

Risk prediction—Homocysteine in Coronary Heart Disease

Selective Thiol Detection in Authentic Biological Samples with the Use of Screen-printed Electrodes

Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection

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