Total glycerol analysis in biodiesel samples using solid phase extraction coupled with enzymatic-spectrophotometric determination

Muniz, Renan de Oliveira; Martins, Sheila Barbosa; Honório, Gláucio Gualtieri; Cunha, Jéssica Nogueira da; Souza, Cristiane Gimenes de; Andrade, Débora França de; Pradelle, Renata Norah Chaar; Turkovics, Franck; Neto, Rafael Serralvo; d’Avila, Luiz Antonio; D’Elia, Eliane

doi:10.1039/c8ay02736f

Cited by 11 publications

(13 citation statements)

References 13 publications

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“…The recovery was evaluated by comparing the average of the triplicates with the theoretical value. The result of repeatability was expressed as the coefficient of variation, also known as the relative standard deviation (RSD%) 57,58 .…”

Section: Recovery and Precisionmentioning

confidence: 99%

Electrochemical Sensor for Ethylene Glycol using Reduced Graphene Oxide/AuNp/Ni(OH)2 Modified Glassy Carbon Electrode

et al. 2021

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An electrochemical sensor based on a modified glassy carbon electrode (GCE) with reduced graphene oxide and Ni-Au nanoparticles (Ni(OH) 2 /AuNp/rGO/GCE) was developed for the determination of ethylene glycol. The graphene oxide was reduced electrochemically at the electrode surface by chronoamperometry, the gold nanoparticles were deposited by chronopotentiometry while the nickel hydroxide nanoparticles were deposited by cyclic voltammetry. The characterization of graphene oxide was performed by Raman spectroscopy, X-ray diffraction (XRD) and transmission-mode scanning electron microscopy (TSEM), while the modified electrodes were characterized by scanning electron microscopy (SEM) and electron dispersive spectroscopy (EDS) analysis. The determination of ethylene glycol was performed by cyclic voltammetry due to the regeneration of the active sites, preventing loss of the sensor signal. The modified GCE with rGO and Ni(OH) 2 /AuNp showed a good performance obtaining a linear range of 0.24 to 1.4 mmol L -1 with a correlation coefficient of 0.9903, limits of detection and quantification (49 and 162 µmol L -1 , respectively) and high stability with 500 continuous analysis cycles.

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Section: Recovery and Precisionmentioning

confidence: 99%

Electrochemical Sensor for Ethylene Glycol using Reduced Graphene Oxide/AuNp/Ni(OH)2 Modified Glassy Carbon Electrode

et al. 2021

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“…Free or total glycerin in biodiesel can be determined by spectrophotometry, since the reactions shown in Figure 8 result in a colored compound, as initially described by Greenhill [37]. Several publications have used similar methods, taking advantage of kits used to analyze triglycerides in blood [36,[38][39][40]. In the presence of adenosine-5'-triphosphate (ATP) and the enzyme glycerol kinase (GK), glycerol produces diphosphate adenosine-5'diphosphate (ADP) and glycerol-3-phosphate (G-3-P; reaction 1), which, in the presence of glycerol phosphate oxidase (GPO) and oxygen, produces dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H 2 O 2 ; reaction 2).…”

Section: Free and Total Glycerinmentioning

confidence: 99%

“…To determine total glycerin, it is necessary to use solid-phase extraction in an aminopropyl cartridge to separate the biodiesel (FAMEs) from the fraction containing the free glycerin (FG), monoacylglycerols (MAGs), diacylglycerols (DAGs), triacylglycerols (TAGs), and combined glycerin, as shown in Figure 9a [36]. To determine free glycerin, the biodiesel (FAME) is separated and isolated from the fraction containing the free glycerin (FG) by solid-phase extraction in a silica cartridge (see Figure 9b).…”

Section: Free and Total Glycerinmentioning

confidence: 99%

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Fuel Quality Monitoring by Color Detection

Santos¹,

Silva²,

Borges³

et al. 2020

Color Detection

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The quality of automotive fuels-gasoline, diesel, biodiesel, and ethanol-is discussed in the light of their specifications in different countries and regions and in terms of their adulteration, which has such a prejudicial effect on the production chain, distribution chain, tax revenues, the environment, and end consumers. Different ways of adulterating automotive fuels are analyzed, as are the procedures for their detection. Several analytical methods for monitoring quality and detecting adulteration have been addressed in the literature, emphasizing the determination of properties such as density, distillation curve, octane rating, vapor pressure, etc., by means of physicochemical methods and chromatographic and spectrographic techniques, to the detriment of colorimetric methods. This chapter looks at colorimetric techniques designed for quality monitoring and the detection of adulteration in fuels, especially simple, quick, low-cost procedures with potential to be used in the field.

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“…There are several methods for the analysis of free glycerol. Among these are chromatographic (the standard method ASTM D6584 [9]), amperometric, spectrophotometric, and enzymatic [7,8,[10][11][12][13][14] methods. However, the most widely and currently studied method is electrochemical, due to its analysis being less laborious, less expensive, and at the same time sensitive and fast.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Analysis of Free Glycerol in Biodiesel Using Reduced Graphene Oxide and Gold/Palladium Core-Shell Nanoparticles Modified Glassy Carbon Electrode

et al. 2021

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Glycerol is a major byproduct obtained in the production of biodiesel, an important renewable fuel. The presence of free glycerol in fuel can have structural and performance consequences with respect to the engine, making fuel quality control important. The standard method to analyze glycerol in biodiesel is gas chromatography, a time-consuming and expensive technique. In this context, an electrode based on glassy carbon electrodes (GCEs) modified with reduced graphene oxide and core-shell gold@palladium nanoparticles was developed for the determination of glycerol in biodiesel. The free glycerol analysis was performed in the aqueous phase obtained by liquid–liquid extraction from a biodiesel sample. Cyclic voltammetry was chosen as the method for glycerol electrochemical analysis to regenerate active sites and promote greater sensor stability. The modified Au@Pd/rGO/GCE electrode showed an excellent performance, obtaining a linear range of 18.2 to 109 µmol L−1 with a correlation coefficient of 0.9895, limits of detection and quantification of 5.33 and 17.6 µmol L−1, respectively, high stability during 1000 cycles, and recovery values of 86% and 87% in the quantification of glycerol in biodiesel samples. The proposed method proved to be a great alternative for the analysis of glycerol in biodiesel, being a fast, sensitive, and low-cost technique due to its high stability and the use of small quantities of reagents.

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Total glycerol analysis in biodiesel samples using solid phase extraction coupled with enzymatic-spectrophotometric determination

Abstract: In this work, a method was developed for the quantification of total glycerol in biodiesel using solid phase extraction (SPE) coupled with enzymatic-spectrophotometric determination.

Cited by 11 publications

References 13 publications

Electrochemical Sensor for Ethylene Glycol using Reduced Graphene Oxide/AuNp/Ni(OH)2 Modified Glassy Carbon Electrode

Electrochemical Sensor for Ethylene Glycol using Reduced Graphene Oxide/AuNp/Ni(OH)2 Modified Glassy Carbon Electrode

Fuel Quality Monitoring by Color Detection

Electrochemical Analysis of Free Glycerol in Biodiesel Using Reduced Graphene Oxide and Gold/Palladium Core-Shell Nanoparticles Modified Glassy Carbon Electrode

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