Total Glucosides of &lt;i&gt;Paeonia lactiflora&lt;/i&gt; Pall Suppress Nitric Oxide Production and iNOS Expression in Lipopolysaccharide-Stimulated RAW264.7 Macrophages

Chen, Gang; Tan, Ming‐Liang; Gao, Xue; Kong, Sue

doi:10.4314/tjpr.v13i8.11

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…. 白灼藥은 諸痛을 치료 하며 7) NO, NOS-II를 억제하고 10) , 細辛은 TNF-α, IL-8, IL-6 등의 분비를 억제시킨다 11) , 그 외 甘草 12) , 大棗 (Fig. 1A, B).…”

Section: ) Ltbunclassified

Effects of Danggwisayeok-tang (Dangguisinitang) on MIA-Induced Osteoarthritis Rats

Yang¹,

Woo²,

Kim³

et al. 2015

Journal of Korean Medicine Rehabilitation

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Objectives The purpose of this study was to know the effects of Danggwisayeok-tang (Dangguisinitang) extract (DGSYT) on monosodium iodoacetate (MIA)-induced rat osteoarthritis.Methods For this purpose, rats were divided into 5 groups. Normal group was not injected with MIA and orally administered any medication. Control group was injected with MIA and not orally administered any medication. DGSYT100 group was injected with MIA and orally administered 100 mg/kg of DGSYT. DGSYT300 group was injected with MIA and orally administered 300 mg/kg of DGSYT. JoinsT group was injected with MIA and orally administered 20 mg/kg of Joins tablet. DGSYT100 and DGSYT300 groups were orally administered DGSYT during a week before and 3 weeks after based on the day MIA injected. The changes of hepatotoxicity, nephrotoxicity, relative hind paw weight distribution, cytokine in serum, cytokine messenger ribonucleic acid (mRNA) in joint tissue and histopathological observation (Hematoxylin & Eosin and Safranin-O staining) were measured. Results Alanine aminotransferase (ALT) levels of DGSYT100, DGSYT300 and JoinsT groups were increased significantly, but these results were within normal range. Aspartate aminotransferase (AST) and creatinine levels of all groups were not changed significantly. In the change of relative hind paw weight distribution, DGSYT300 and JoinsT groups were decreased significantly 14 and 21 days after MIA injected. Interleukin-1β (IL-1β) and Interleukin-6 (IL-6), Leukotriene B4 and Osteocalcin levels of DGSYT300 and JoinsT groups were decreased significantly. In measurement of IL-1β and nitric oxide synthase-Ⅱ mRNA relative quantitative of control, DGSYT100, DGSYT300 and JoinsT groups were decreased significantly. In measurement of TNF-α, IL-6 and Cyclooxygenase-2 mRNA relative quantitative of control, DGSYT300 and JoinsT groups was decreased significantly. In histopathological observation of knee, synovial tissue, cartilage and proteoglycan of DGSYT100, DGSYT300 and JoinsT were well preserved compared with control group. Conclusions According to the results, DGSYT has anti-inflammation and pain relief effects. So it should be suppressed progression of arthritis in MIA-induced osteoarthritis rat. (J Korean Med Rehab 2015;25(2):37-50)

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“…. 白灼藥은 諸痛을 치료 하며 7) NO, NOS-II를 억제하고 10) , 細辛은 TNF-α, IL-8, IL-6 등의 분비를 억제시킨다 11) , 그 외 甘草 12) , 大棗 (Fig. 1A, B).…”

Section: ) Ltbunclassified

Effects of Danggwisayeok-tang (Dangguisinitang) on MIA-Induced Osteoarthritis Rats

Yang¹,

Woo²,

Kim³

et al. 2015

Journal of Korean Medicine Rehabilitation

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Bidirectional immunomodulating activity of fermented polysaccharides from Yupingfeng

Sun

Zeng

Zou

et al. 2017

Research in Veterinary Science

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Antioxidant, Anti-inflammatory and Cartilage Regeneration Effects of Euiiin-tang

Park,

Kim,

Shon

et al. 2023

J Korean Med Rehabil

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Objectives The purpose of this study was to investigate the antioxidant, anti-inflammatory and cartilage regeneration effects of Euiiin-tang water extract (EIIT) in the treatment of monosodium iodoacetate (MIA)-induced osteoarthritis in rats. Methods Animal models were divided into five groups. The normal group didn't do any treatments causing osteoarthritis. The control group was orally administerd distilled water instead of the drug, the positive control group used indomethacin 5 mg/kg, the EIIT 100 group used EIIT 100 mg/kg and the EIIT 200 group used EIIT 200 mg/kg, and seven rats were placed per group. We administered drug to rats for 2 weeks and analyzed oxidative stress-related proteins in joint tissue. Inflammation mediators and inflammatory cytokines induced by the activity of inflammation-related proteins were analyzed. In addition, the expression of anti-inflammatory cytokines and collagen-related factors were analyzed, and H&E staining and Safranin-O staining were performed to see the effect on histopathological changes. Results 1) Oxidative stress-related proteins were significantly reduced. 2) Inflammationrelated proteins, inflammatory mediators and inflammatory cytokines were significantly reduced. 3) Anti-inflammatory cytokines were significantly increased. 4) Collagen proteolysis factors significantly decreased, and collagen degradation inhibitory factor was significantly increased. 5) EIIT administration significantly reduced cartilage degeneration and deformation in H&E staining, and reduced proteoglycan destruction in Safranin-O staining. Conclusions From the above experimental results, it judges that Euiiin-tang has antioxidant, anti-inflammatory, and cartilage regeneration effects on osteoarthritis in rats induced by MIA.

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Total Glucosides of <i>Paeonia lactiflora</i> Pall Suppress Nitric Oxide Production and iNOS Expression in Lipopolysaccharide-Stimulated RAW264.7 Macrophages

Abstract: Purpose: To investigate the effect of total glucosides of Paeonia lactiflora (TGPL) on nitric oxide (NO) production and its potential mechanism in lipopolysaccharide (LPS

Cited by 3 publications

References 13 publications

Effects of Danggwisayeok-tang (Dangguisinitang) on MIA-Induced Osteoarthritis Rats

Effects of Danggwisayeok-tang (Dangguisinitang) on MIA-Induced Osteoarthritis Rats

Bidirectional immunomodulating activity of fermented polysaccharides from Yupingfeng

Antioxidant, Anti-inflammatory and Cartilage Regeneration Effects of Euiiin-tang

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