Total Flavonoids of Scutellaria barbata Inhibit Invasion of Hepatocarcinoma via MMP/TIMP in Vitro

Dai, Zhijun; Wang, Baofeng; Lu, Wang-Feng; Wang, Zhidong; Ma, Xiaolong; Wang, Min; Kang, Huafeng; Wang, Xijing; Wang, Wenying

doi:10.3390/molecules18010934

Cited by 35 publications

(23 citation statements)

References 36 publications

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“…Scutellaria barbata D. Don (SB) (Figure 1A ), which is derived from the dried whole plant of Labiatae, is a well-known herb and drug in traditional Chinese Medicine where it is used as an anti-inflammatory herb. In recent years, some researchers have demonstrated that SB has significant antitumor activity in breast cancer [ 13 , 14 ], colorectal cancer [ 15 - 18 ], hepatocarcinoma [ 19 - 21 ], uterine leiomyoma [ 22 , 23 ], cervix cancer [ 24 ], skin cancer [ 25 ] and lung cancer [ 26 - 28 ]. However, the precise mechanism of the anti-tumor effect of SB in lung cancer is not yet clear.…”

Section: Introductionmentioning

confidence: 99%

The anti-cancer effects and mechanisms of Scutellaria barbata D. Don on CL1-5 lung cancer cells

et al. 2017

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Lung cancer, with a poor prognosis and resistance to chemotherapy, is the most common malignant tumor and has the highest mortality rate worldwide. Scutellaria barbata D. Don (SB), which is derived from the dried whole plant of Labiatae, is a well-known anti-inflammatory and anti-cancer herb. The aim of this study was to examine the anti-cancer effects and precise regulatory mechanisms of SB in CL1-5 lung cancer cells. In an in vitro assay, we found that the anti-tumor mechanism of SB was due to P38/SIRT1-regulated cell apoptosis through G2/M phase arrest and ER stress-, intrinsic mitochondrial-, and extrinsic FAS/FASL-mediated pathways. Autophagy also plays a key role in SB-induced CL1-5 cell cytotoxicity. In addition, SB exerts additive effects with etoposide or cisplatin in lung cancer cells. In an in vivo assay, we found that SB significantly reduces tumor size with decreased proliferation and angiogenesis, as well as increased apoptosis and autophagy in CL1-5 tumor-bearing mice. These findings provided experimental evidence for the application of SB in the treatment of lung cancer.

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Section: Introductionmentioning

confidence: 99%

The anti-cancer effects and mechanisms of Scutellaria barbata D. Don on CL1-5 lung cancer cells

et al. 2017

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“…The research showed that the flavonoids of SB had significant antitumor activity due to the enhancement of the immune function [ 13 ]. Flavonoids of SB including scutellarin, naringin, apigenin, luteolin, and wogonin have been found to significantly inhibit the proliferation and invasion of MHCC97H cells in a dose-dependent manner [ 14 ]. Yang et al demonstrated that scutebarbatine A possessed significant antitumor effects on A549 cells in vitro and transplanted tumor nude mice in vivo via mitochondria-mediated apoptosis [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines ofScutellaria barbataD. Don in Lewis-Bearing C57BL/6 Mice

Gong

Wang

Yuan

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Immunomodulatory effect has been found to be an important therapeutic measure for immune responses against cancer. In this study, we evaluated the inhibition of Scutellaria barbata D. Don (SB), an anti-inflammatory and an antitumor Chinese herb, including flavonoids and scutebarbatines on tumor growth and its immunomodulatory effects in vivo. HPLC and LC/MS/MS methods were conducted for the analysis of flavonoids and scutebarbatines in SB. Lewis-bearing C57BL/6 mice model was established and tumor volume was evaluated by high frequency color ultrasound experiment. ELISA and western blot analysis were performed for the determination of immunomodulatory factors. SB treatment at the dose of 10, 6.67, and 3.33 g crude drug/kg/d significantly inhibited tumor growth of Lewis-bearing C57BL/6 mice with the inhibition rates of 44.41 ± 5.44%, 33.56 ± 4.85%, and 27.57 ± 4.96%, respectively. More importantly, the spleen and thymus indexes were increased remarkably by SB treatment. SB could decrease IL-17, IL-10, FOXP3, TGF-β1, RORγt, and IL-6 levels whereas it could increase remarkably IL-2 and IFN-γ levels. Our results demonstrated that SB could inhibit tumor growth in vivo through regulating immune function in tumor-bearing mice and suggested that the immunomodulatory function of SB had a potential therapeutic effect in lung cancer.

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“…In addition, S. barbata also exhibited anti‐tumor effects on liver cancer . The flavonoids in S. barbata reduced the invasion of hepatocarcinoma cells by inhibiting the angiogenesis. The flavonoids responsible for this activity are including scutellarin, baicalin, luteolin, naringin, wogonin, and apigenin …”

Section: Resultsmentioning

confidence: 99%

Constituents from Scutellaria barbata Inhibiting Nitric Oxide Production in LPS‐Stimulated Microglial Cells

Lee

Kim

et al. 2017

Chemistry & Biodiversity

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The arial parts of Scutellaria barbata D. Don (Lamiaceae) efficiently inhibited NO production in BV2 microglial cells, and the active constituents were further isolated based on activity-guided isolation using silica-gel column chromatography, RP-C18 MPLC and prep-HPLC. As the results, 2 flavonoids including 6-methoxynaringenin (1) and 6-O-methylscutellarein (5), and 6 neo-clerodane diterpenes such as scutebarbatine W (2), scutebatas B (3), scutebarbatine B (4), scutebarbatine A (6), 6-O-nicotinolylscutebarbatine G (7), and scutebarbatine X (8) were isolated. The structures of these compounds were elucidated based on NMR and MS data, and the comparison of literature values. All the compounds except compound 7 inhibited NO production efficiently with IC values of lower than 50 μm. Particularly, compounds 1 and 8 were the most efficient with IC values of 25.8 and 27.4 μm, respectively. This is the first report suggesting the potential of S. barbata on the reduction of neuroinflammation.

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Total Flavonoids of Scutellaria barbata Inhibit Invasion of Hepatocarcinoma via MMP/TIMP in Vitro

Cited by 35 publications

References 36 publications

The anti-cancer effects and mechanisms of Scutellaria barbata D. Don on CL1-5 lung cancer cells

The anti-cancer effects and mechanisms of Scutellaria barbata D. Don on CL1-5 lung cancer cells

Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines ofScutellaria barbataD. Don in Lewis-Bearing C57BL/6 Mice

Constituents from Scutellaria barbata Inhibiting Nitric Oxide Production in LPS‐Stimulated Microglial Cells

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