Total flavonoids of Rhizoma Drynariae protect hepatocytes against aflatoxin B1-induced oxidative stress and apoptosis in broiler chickens

Lin, Luxi; Fu, Pengfei; Chen, Nuoman; Gao, Ningyu; Cao, Qi; Ke, Yanxiong; Xu, Tingting; Zhang, Chaodong; Cai, Zaisheng; Liu, Fang; Xuebing, Wang; Huang, Shucheng

doi:10.1016/j.ecoenv.2021.113148

Cited by 28 publications

(28 citation statements)

References 40 publications

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“…The increased liver index might be ascribed to AFB 1, resulting in inflammatory enlargement of the liver and/or metabolic disordered lipid accumulation [ 9 ]. AFB 1 also caused histopathological changes and an increase in apoptosis of the liver, which is consistent with the findings of former studies [ 13 , 20 , 40 , 43 ], suggesting that AFB 1 induced liver injury in broilers.…”

Section: Discussionsupporting

confidence: 92%

“…Although the production performance was not affected by AFB 1 , it was observed that the serum ALT and AST activity and the relative liver weight were increased in broilers after being subjected to AFB 1 exposure. The liver is considered to be the main target organ for AFB 1 attack; relevant studies also confirmed that AFB 1 -exposed chickens had a higher liver index and increased serum ALT and AST activity [ 19 , 40 , 41 ]. It is well known that ALT and AST are commonly used as clinical biomarkers of liver function; the presence of elevated ALT and AST activity indicates abnormal liver function [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Hepatic Detoxification Effects of Enteromorpha prolifera Polysaccharides against Aflatoxin B1 in Broiler Chickens

Yang

Pushparaj

et al. 2022

Antioxidants

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Aflatoxin B1 (AFB1) is a major risk factor in animal feed. Seaweed (Enteromorpha prolifera)-derived polysaccharides (SDP) are natural antioxidants with multiple biological functions, which may have an in vivo detoxification effect on AFB1. The current study aimed to evaluate whether SDP could mitigate AFB1-induced hepatotoxicity in broilers. A total of 216 chickens (male, 5 weeks old) were randomly allocated to three groups with differing feeding patterns, lasting 4 four weeks: (1) control group (CON, fed a basal diet); (2) AFB1 group (fed a basal diet mixed with 0.1 mg/kg AFB1); and (3) AFB1 + SDP group (AFB1 group + 0.25% SDP). The results showed that dietary SDP improved the liver function-related biochemical indicators in serum, and reversed the increase in relative liver weight, hepatic apoptosis and histological damage of broilers exposed to AFB1. SDP treatment also reduced the activity and mRNA expression of phase I detoxification enzymes, while increasing the activity and mRNA expression of phase II detoxification enzymes in the livers of AFB1-exposed broilers, which was involved in the activation of p38 mitogen-activated protein kinase (p38MAPK)/nuclear factor erythroid 2-related factor 2 (Nrf2) signaling. In conclusion, dietary SDP alleviated AFB1-induced liver injury of broilers through inhibiting phase I detoxification enzymes and upregulating p38MAPK/Nrf2-mediated phase II detoxification enzymes pathway.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Evaluation of Hepatic Detoxification Effects of Enteromorpha prolifera Polysaccharides against Aflatoxin B1 in Broiler Chickens

Yang

Pushparaj

et al. 2022

Antioxidants

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“…The qRT-PCR was performed according to the SYBR Green I PCR Master Mix (Vazyme Biotech Co., Ltd., China). The PCR amplification thermal cycling consisted of predeformation at 95°C for 30 s, followed by 40 cycles consisting of denaturation at 95°C for 10 s, extension at 72°C for 30 s, and annealing at 60°C for 30 s. Calculated according to the expression of mRNA using the 2 −ΔΔCT method ( Lin et al, 2022 ). All the assays were performed three times to ensure accuracy.…”

Section: Methodsmentioning

confidence: 99%

Total Flavonoids of Rhizoma Drynariae Ameliorate Bone Growth in Experimentally Induced Tibial Dyschondroplasia in Chickens via Regulation of OPG/RANKL Axis

Zheng

Jin

et al. 2022

Front. Pharmacol.

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Background:Rhizoma Drynariae, traditional Chinese herb, is widely used to treat and prevent bone disorders. However, experimental evidence on the use of Rhizoma Drynariae extract, total flavonoids of Rhizoma Drynariae (TFRD) to treat tibial dyschondroplasia (TD) in chickens and its underlying mechanisms have not been investigated.Purpose: To evaluate the therapeutic effect of TFRD on leg disease caused by TD and elucidate its mechanisms in modulating the bone status.Methods: Thiram-induced chicken TD model has been established. The tibia status was evaluated by analyzing tibia-related parameters including tibial weight, tibial length and its growth plate width and by performing histopathological examination. The expression of tibial bone development-related genes and proteins was confirmed by western blotting and qRT-PCR.Results: The results showed that administration of TFRD mitigated lameness, increased body weight, recuperated growth plate width in broilers affected by TD and the increase of tibia weight and tibia length is significantly positively correlated with body weight. Compared with the TD group broilers, 500 mg/kg TFRD evidently reduced the damage width of the growth plate and improved its blood vessel distribution by elevating the gene expression levels of BMP-2 and Runx2 and OPG/RANKL ratio. Furthermore, correlation analysis found that the damage width of the growth plate was negatively correlated with the expression levels of BMP-2 and OPG.Conclusion: The present study revealed that TFRD could promote the bone growth via upregulating OPG/RANKL ratio, suggesting that TFRD might be a potential novel drug in the treatment of TD in chickens.

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“…Antioxidants are additives supplemented with feed primarily for prolonging the shelf life and sensory qualities, preventing rancidity, and the oxidation of critical nutrients such as pigments and vitamins. Antioxidants do not bind or modify mycotoxins, but they can counteract the toxic effects of mycotoxins by targeting oxidative stress and inflammatory signalling pathways to alleviate aflatoxin-induced toxicities [ 19 , 37 ]. Aflatoxins inhibit the expression of antioxidant enzymes, including superoxide dismutase and glutathione peroxidase resulting in the overproduction of free radicals or reactive oxygen species (ROS) [ 8 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Additives for the Mitigation of Aflatoxins in Animal Feed: A Systematic Review and Network Meta-Analysis

Kolawole

Siri-anusornsak

Petchkongkaew

et al. 2022

Toxins

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The contamination of animal feed with aflatoxins is an ongoing and growing serious issue, particularly for livestock farmers in tropical and subtropical regions. Exposure of animals to an aflatoxin-contaminated diet impairs feed efficiency and increases susceptibility to diseases, resulting in mortality, feed waste, and increased production costs. They can also be excreted in milk and thus pose a significant human health risk. This systematic review and network meta-analysis aim to compare and identify the most effective intervention to alleviate the negative impact of aflatoxins on the important livestock sector, poultry production. Eligible studies on the efficacy of feed additives to mitigate the toxic effect of aflatoxins in poultry were retrieved from different databases. Additives were classified into three categories based on their mode of action and composition: organic binder, inorganic binder, and antioxidant. Moreover, alanine transaminase (ALT), a liver enzyme, was the primary indicator. Supplementing aflatoxin-contaminated feeds with different categories of additives significantly reduces serum ALT levels (p < 0.001) compared with birds fed only a contaminated diet. Inorganic binder (P-score 0.8615) was ranked to be the most efficient in terms of counteracting the toxic effect of aflatoxins, followed by antioxidant (P-score 0.6159) and organic binder (P-score 0.5018). These findings will have significant importance for farmers, veterinarians, and animal nutrition companies when deciding which type of additives to use for mitigating exposure to aflatoxins, thus improving food security and the livelihoods of smallholder farmers in developing countries.

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Total flavonoids of Rhizoma Drynariae protect hepatocytes against aflatoxin B1-induced oxidative stress and apoptosis in broiler chickens

Cited by 28 publications

References 40 publications

Evaluation of Hepatic Detoxification Effects of Enteromorpha prolifera Polysaccharides against Aflatoxin B1 in Broiler Chickens

Evaluation of Hepatic Detoxification Effects of Enteromorpha prolifera Polysaccharides against Aflatoxin B1 in Broiler Chickens

Total Flavonoids of Rhizoma Drynariae Ameliorate Bone Growth in Experimentally Induced Tibial Dyschondroplasia in Chickens via Regulation of OPG/RANKL Axis

The Efficacy of Additives for the Mitigation of Aflatoxins in Animal Feed: A Systematic Review and Network Meta-Analysis

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