Total Flavonoids of Chuju Decrease Oxidative Stress and Cell Apoptosis in Ischemic Stroke Rats: Network and Experimental Analyses

Chen, Hao; Jiang, Huihui; Mao, Bin-bin; Yu, Hao

doi:10.3389/fnins.2021.772401

Cited by 9 publications

(7 citation statements)

References 71 publications

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“…In addition, flavonoids can exert an effect on the expression, as well as on the activity of apoptotic proteins such as BCL-2, BAX, and cleaved caspase-3 (CASP3), which ultimately leads to the induction of apoptosis in cancer cells. Polyphenols are found in a variety of plant-based foods and beverages [ 33 , 35 ]. They can influence the process of PI3K/AKT/mTOR signaling, which is necessary for the survival of cells, the proliferation of cells, the metabolism of cells, and angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Possible Pathways Involved in the Protective Effects of Quercetin, Naringenin, and Rutin at the Gene, Protein and miRNA Levels Using In-Silico Multidimensional Data Analysis

et al. 2023

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Flavonoids are secondary metabolites that are non-essential for plant growth or survival, and they also provide numerous health benefits to humans. They are antioxidants that shield plants from the ill effects of ultraviolet light, pests, and diseases. They are beneficial to health for several reasons, including lowering inflammation, boosting cardiovascular health, and lowering cancer risk. This study looked into the physicochemical features of these substances to determine the potential pharmacological pathways involved in their protective actions. Potential targets responsible for the protective effects of quercetin, naringenin, and rutin were identified with SwissADME. The associated biological processes and protein–protein networks were analyzed by using the GeneMANIA, Metascape, and STRING servers. All the flavonoids were predicted to be orally bioavailable, with more than 90% targets as enzymes, including kinases and lyases, and with common targets such as NOS2, CASP3, CASP9, CAT, BCL2, TNF, and HMOX1. TNF was shown to be a major target in over 250 interactions. To extract the “biological meanings” from the MCODE networks’ constituent parts, a GO enrichment analysis was performed on each one. The most important transcription factors in gene regulation were RELA, NFKB1, PPARG, and SP1. Treatment with quercetin, naringenin, or rutin increased the expression and interaction of the microRNAs’ hsa-miR-34a-5p, hsa-miR-30b-5p, hsa-let-7a-5p, and hsa-miR-26a-1-3p. The anticancer effects of hsa-miR-34a-5p have been experimentally confirmed. It also plays a critical role in controlling other cancer-related processes such as cell proliferation, apoptosis, EMT, and metastasis. This study’s findings might lead to a deeper comprehension of the mechanisms responsible for flavonoids’ protective effects and could present new avenues for exploration.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Possible Pathways Involved in the Protective Effects of Quercetin, Naringenin, and Rutin at the Gene, Protein and miRNA Levels Using In-Silico Multidimensional Data Analysis

et al. 2023

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“…It catalyzes the iterative condensation and intramolecular cyclization of one molecule of p -coumaroyl-CoA and three molecules of malonyl-CoA to produce naringenin chalcone, which is the basic flavonoid skeleton . Molecular modification by downstream isomerases, hydroxylases, reductases, glycosyltransferases, and acyltransferases biosynthesizes a wide range of flavonoids from naringenin chalcone. , Naringenin chalcone and its derived flavonoids are widely distributed in plants and constitute a major class of natural products, including more than 15000 compounds. , Flavonoids have numerous applications in medicine, agrochemicals, cosmetics, food, and the chemical industry because of their various physiological activities, such as antiatherosclerotic, oxidation resistance, liver protection, and anticarcinogenic. , CHS has become a focus of research attention because of its essential function in the flavonoid biosynthetic pathway …”

Section: Introductionmentioning

confidence: 99%

“…5,6 Flavonoids have numerous applications in medicine, agrochemicals, cosmetics, food, and the chemical industry because of their various physiological activities, such as antiatherosclerotic, oxidation resistance, liver protection, and anticarcinogenic. 7,8 CHS has become a focus of research attention because of its essential function in the flavonoid biosynthetic pathway. 9 CHS is the rate-limiting enzyme in flavonoid biosynthesis, and CHS activity was mathematically correlated with the accumulation of flavonoids in plants during the organogenesis of primary oat leaves.…”

Section: ■ Introductionmentioning

confidence: 99%

Improvement of Chalcone Synthase Activity and High-Efficiency Fermentative Production of (2S)-Naringenin via In Vivo Biosensor-Guided Directed Evolution

Tong,

Li,

Zhou

et al. 2024

ACS Synth. Biol.

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Chalcone synthase (CHS) catalyzes the rate-limiting step of (2S)-naringenin (the essential flavonoid skeleton) biosynthesis. Improving the activity of the CHS by protein engineering enhances (2S)-naringenin production by microbial fermentation and can facilitate the production of valuable flavonoids. A (2S)-naringenin biosensor based on the TtgR operon was constructed in Escherichia coli and its detection range was expanded by promoter optimization to 0−300 mg/L, the widest range for (2S)-naringenin reported. The high-throughput screening scheme for CHS was established based on this biosensor. A mutant, SjCHS1 S208N with a 2.34-fold increase in catalytic activity, was discovered by directed evolution and saturation mutagenesis. A pathway for de novo biosynthesis of (2S)-naringenin by SjCHS1 S208N was constructed in Saccharomyces cerevisiae, combined with CHS precursor pathway optimization, increasing the (2S)-naringenin titer by 65.34% compared with the original strain. Fed-batch fermentation increased the titer of (2S)-naringenin to 2513 ± 105 mg/L, the highest reported so far. These findings will facilitate efficient flavonoid biosynthesis and further modification of the CHS in the future.

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“…Proteins, DNA, and fats in cells undergo crucially significant modifications as a consequence of excessive ROS production [13]. Several investigations have demonstrated a strong association between OS and neurological conditions such as stroke, AD, and TBI [14–17]. An essential transcription agent called nuclear factor erythroid 2 related factor 2 (Nrf2) is crucial in the fight against OS.…”

Section: Introductionmentioning

confidence: 99%

“…Along with cherries and other berries, apples have the greatest concentration of Que among fruits [33, 34]. Que is a healthy dietary component with few side effects [15]. The elimination of ROS is induced by the Que therapy, and brain tissues are protected from OS, according to other reports [35, 36].…”

Section: Introductionmentioning

confidence: 99%

Targeting Nrf2 signaling pathway by quercetin in the prevention and treatment of neurological disorders: An overview and update on new developments

Zamanian

Soltani

Khodarahmi

et al. 2023

Fundamemntal Clinical Pharma

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Background: Neurological disorders (NLDs) are widely acknowledged as a significant public health concern worldwide. Stroke, Alzheimer's disease (AD), and traumatic brain injury (TBI) are three of these disorders that have sparked major study attention. Neurological dysfunction, protein buildup, oxidation and neuronal injury, and aberrant mitochondria are all prevalent neuropathological hallmarks of these disorders. The signaling cascade of nuclear factor erythroid 2 related factor 2 (Nrf2) shares all of them as a common target. Several studies have found that overexpression of Nrf2 is a promising treatment method in NLDs. Effective treatment of these disorders continues to be a universal concern regardless of various medicines. In order to treat a variety of neurological problems, organic remedies may provide an alternative treatment. It has been demonstrated that polyphenols like quercetin (Que) offer considerable capabilities for treating NLDs. One of Que's greatest key targets, Nrf2, has the capacity to control the production of a number of cytoprotective enzymes that exhibit neuroprotective, detoxifying, and antioxidative effects. Additionally, Que enhanced the expression of Nrf2 and inhibited alterations in the shape and death of neurons in the hippocampus. Objective: In this review, we have focused on Que's medicinal prospects as a neuroprotective drug. Methods: PubMed, Scopus, Science Direct, and Google Scholar were used to search articles for this study. Results: The findings of this research demonstrate that (1) Que protected the blood‐brain barrier via stimulating Nrf2 in animal stroke, which alleviated ischemic reperfusion and motor dysfunction. (2) By triggering the Nrf2 pathway, Que reduced the neuroinflammation and oxidative damage brought on by TBI in the cortex. (3) In an experimental model of AD, Que enhanced cognitive function by decreasing A1‐4, antioxidant activity, and Nrf2 levels in the brain. Conclusion: We discuss recent research on Que‐mediated Nrf2 expression in the management of several NLDs in this paper.

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Total Flavonoids of Chuju Decrease Oxidative Stress and Cell Apoptosis in Ischemic Stroke Rats: Network and Experimental Analyses

Cited by 9 publications

References 71 publications

Evaluation of the Possible Pathways Involved in the Protective Effects of Quercetin, Naringenin, and Rutin at the Gene, Protein and miRNA Levels Using In-Silico Multidimensional Data Analysis

Evaluation of the Possible Pathways Involved in the Protective Effects of Quercetin, Naringenin, and Rutin at the Gene, Protein and miRNA Levels Using In-Silico Multidimensional Data Analysis

Improvement of Chalcone Synthase Activity and High-Efficiency Fermentative Production of (2S)-Naringenin via In Vivo Biosensor-Guided Directed Evolution

Targeting Nrf2 signaling pathway by quercetin in the prevention and treatment of neurological disorders: An overview and update on new developments

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