Total flavonoids of Chrysanthemum indicum L inhibit acute pancreatitis through suppressing apoptosis and inflammation

Abstract

Acute pancreatitis (AP) is one of the most common acute abdomen. Inflammation and apoptosis are closely linked with AP development. Total flavonoids of Chrysanthemum indicum L (TFC) has been proved to inhibit inflammation and apoptosis. If TFC could suppress AP remains unclear. AP animal and cell models were established with Cerulein. The pancreatic tissue injury was measured with HE staining. Inflammatory factors were detected with ELISA method. The protein expression was evaluated with Western blotting. Inhibition of AP in vivo was achieved by TFC by inhibiting serum amylase, myeloperoxidase (MPO), and water content of pancreatic tissue. The increased inflammatory response and activation of NF-κB signaling pathway in AP rats were inhibited after TFC treatment. The activation of NF-κB signaling pathway, increase of cell apoptosis and inflammatory factors in AR42J cells were suppressed by TFC. We demonstrated that TFC could significantly inhibit AP through restraining serum amylase, MPO, water content of pancreatic tissue, inflammation levels, apoptosis, and NF-κB signaling pathway activation. This study might clarify the potential inhibition mechanism of TFC in AP development.

Material basis and molecular mechanisms of Chaihuang Qingyi Huoxue Granule in the treatment of acute pancreatitis based on network pharmacology and molecular docking-based strategy

“…Currently, drugs specific to AP are lacking, and most available treatments focus on symptom relief and supportive care (26). TCM is a highly effective method for treating AP due to its various benefits, including its ability to target multiple areas and its minimal adverse reactions (28,29). Previous animal and clinical experiments have confirmed the therapeutic impact of CH on AP, yet the precise molecular mechanisms behind it remain unclear.…”

Identification of glycosyltransferase genes for diagnosing T-cell mediated rejection and predicting graft loss in kidney transplantation

“…IL6, when bound to its receptor, can initiate a cascade through JAK activation, and activated JAK kinase phosphorylates STAT3, inducing dimerization, leading to in ammation [43]. TNFA can activate NFKB, further causing a magni ed in ammatory reaction [44]. During an immune response, activated CD4 + and CD8 + T lymphocytes secrete large quantities of IL2.…”

Prevention of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linnén on cutaneous squamous cell carcinomas progression following UV irradiation in mice

“…Chrysanthemum indicum Linnén (C. indicum) is a medicinal and food herb, which is readily available in East Asia (22). Previous studies have reported that C. indicum may have antihypertensive, antioxidant, antiseptic and anticancer properties, as well as inhibiting lipogenesis (22)(23)(24)(25). Supercritical carbon dioxide fluid extraction has been successfully employed for the extraction process of flowers and buds of C. indicum because it can ensure a high extraction rate and the structural integrity of its volatile compounds, such as monoterpenes, sesquiterpenes and alkenes (26).…”