Total flavonoids from Potentilla kleiniana Wight et Arn inhibits biofilm formation and virulence factors production in methicillin-resistant Staphylococcus aureus (MRSA)

Tao, Junyu; Yan, Shilun; Zhou, Chuyue; Liu, Qiong; Zhu, Huaqun; Wen, Zhen

doi:10.1016/j.jep.2021.114383

Cited by 11 publications

(10 citation statements)

References 52 publications

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“…Recently, Song et al [ 14 ] reported that the ethyl acetate extract of P. kleiniana Wight et Arn inhibited E. coli , P. aeruginosa , and C. albicans , with MICs of 5 mg/mL, 2.5 mg/mL, and 5 mg/mL, respectively. Tao et al reported the MIC value of the TFP against MRSA was 20 μg/mL [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

“…Flavonoids, such as the rutin identified in this study, could exert antibacterial activity via damaging the cytoplasmic membrane, inhibiting energy metabolism and synthesis of nucleic acids [ 34 ]. Tao et al also reported the major compounds of the TFP were 3-O-methylducheside A, naringenin, rutin and quercetin [ 9 , 13 ]. Phenols, such as the p-octopamine identified in this study, are potent antibacterial agents against both Gram-positive and Gram-negative bacteria via the disruption of the bacterial membrane, leading to bacterial lysis and leakage of intracellular contents [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…P. kleiniana Wight et Arn is widely distributed in China, and many Asian countries such as Japan, India, Malaysia, Indonesia, and North Korea. Its whole plant has been used as a traditional Chinese medicine to treat fever, arthritis, malaria, insect and snake bites, hepatitis, and traumatic injury [ 9 ]. Recently, Zhou et al identified bioactive components from P. kleiniana Wight et Arn with anti-human immunodeficiency virus-1 (HIV-1) protease activity [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, to the best of our knowledge, there are few studies so far in the current literature on antibacterial activity of P. kleiniana Wight et Arn. Tao et al [ 9 ] reported that total flavonoids from P. kleiniana Wight et Arn (TFP) inhibited biofilm formation and virulence factor production in methicillin-resistant Staphylococcus aureus (MRSA). The TFP also damaged cell membrane integrity of Pseudomonas aeruginosa .…”

Section: Introductionmentioning

confidence: 99%

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Identification of Antibacterial Components and Modes in the Methanol-Phase Extract from a Herbal Plant Potentilla kleiniana Wight et Arn

Tang

Pan

Chen

2023

Foods

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The increase in bacterial resistance and the decline in the effectiveness of antimicrobial agents are challenging issues for the control of infectious diseases. Traditional Chinese herbal plants are potential sources of new or alternative medicine. Here, we identified antimicrobial components and action modes of the methanol-phase extract from an edible herb Potentilla kleiniana Wight et Arn, which had a 68.18% inhibition rate against 22 species of common pathogenic bacteria. The extract was purified using preparative high-performance liquid chromatography (Prep-HPLC), and three separated fragments (Fragments 1–3) were obtained. Fragment 1 significantly elevated cell surface hydrophobicity and membrane permeability but reduced membrane fluidity, disrupting the cell integrity of the Gram-negative and Gram-positive pathogens tested (p < 0.05). Sixty-six compounds in Fragment 1 were identified using Ultra-HPLC and mass spectrometry (UHPLC-MS). The identified oxymorphone (6.29%) and rutin (6.29%) were predominant in Fragment 1. Multiple cellular metabolic pathways were altered by Fragment 1, such as the repressed ABC transporters, protein translation, and energy supply in two representative Gram-negative and Gram-positive strains (p < 0.05). Overall, this study demonstrates that Fragment 1 from P. kleiniana Wight et Arn is a promising candidate for antibacterial medicine and food preservatives.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Antibacterial Components and Modes in the Methanol-Phase Extract from a Herbal Plant Potentilla kleiniana Wight et Arn

Tang

Pan

Chen

2023

Foods

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“…Several studies have reported the antibiofilm effects of flavonoids and their derivatives on bacteria and fungi. They are potential inhibitors of biofilm formation due to their ability to reduce the extracellular matrix [ 19 , 20 ], and they also inhibit the aggregation and maturation of the biofilm by decreasing surface hydrophobicity [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Flavonoid-Rich Fractions of Bauhinia holophylla Leaves Inhibit Candida albicans Biofilm Formation and Hyphae Growth

Fonseca

Teixeira

Ferreira

et al. 2022

Plants

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This study evaluated the effect of the extract and fractions of Bauhinia holophylla on Candida albicans planktonic growth, biofilm formation, mature biofilm, and hyphae growth. Three C. albicans strains (SC5314, ATCC 18804, and ATCC 10231) were tested. The crude extract and the fractions were obtained by exhaustive percolation and liquid–liquid partition, respectively. Phytochemical analyses of B. holophylla extract and fractions were performed using high-performance liquid chromatography coupled with a diode-array detector and mass spectrometry (HPLC-DAD-MS). A microdilution assay was used to evaluate the effect of the B. holophylla extract and fractions on C. albicans planktonic growth, and crystal violet staining was used to measure the total biomass of the biofilm. Hyphae growth was analyzed using light microscopy. Thirteen flavonoids were identified, with a predominance of the flavonol-3-O-glycoside type based on quercetin, myricetin, and kaempferol. Flavonoid-rich fractions of B. holophylla leaves displayed antifungal activity and inhibited both biofilm formation and hyphae growth in all the tested strains, but were not effective on C. albicans planktonic growth and mature biofilm. This study indicates that flavonoid-rich fractions from B. holophylla leaves interfere with the virulence of Candida species and support the use of Bauhinia spp. in folk medicine to treat infections.

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Plant‐derived virulence arresting drugs as novel antimicrobial agents: Discovery, perspective, and challenges in clinical use

Lu,

Wang,

Wang

et al. 2023

Phytotherapy Research

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Antimicrobial resistance (AMR) emerges as a severe crisis to public health and requires global action. The occurrence of bacterial pathogens with multi‐drug resistance appeals to exploring alternative therapeutic strategies. Antivirulence treatment has been a positive substitute in seeking to circumvent AMR, which aims to target virulence factors directly to combat bacterial infections. Accumulated evidence suggests that plant‐derived natural products, which have been utilized to treat infectious diseases for centuries, can be abundant sources for screening potential virulence‐arresting drugs (VADs) to develop advanced therapeutics for infectious diseases. This review sums up some virulence factors and their actions in various species of bacteria, as well as recent advances pertaining to plant‐derived natural products as VAD candidates. Furthermore, we also discuss natural VAD‐related clinical trials and patents, the perspective of VAD‐based advanced therapeutics for infectious diseases and critical challenges hampering clinical use of VADs, and genomics‐guided identification for VAD therapeutic. These newly discovered natural VADs will be encouraging and optimistic candidates that may sustainably combat AMR.

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Total flavonoids from Potentilla kleiniana Wight et Arn inhibits biofilm formation and virulence factors production in methicillin-resistant Staphylococcus aureus (MRSA)

Cited by 11 publications

References 52 publications

Identification of Antibacterial Components and Modes in the Methanol-Phase Extract from a Herbal Plant Potentilla kleiniana Wight et Arn

Identification of Antibacterial Components and Modes in the Methanol-Phase Extract from a Herbal Plant Potentilla kleiniana Wight et Arn

Flavonoid-Rich Fractions of Bauhinia holophylla Leaves Inhibit Candida albicans Biofilm Formation and Hyphae Growth

Plant‐derived virulence arresting drugs as novel antimicrobial agents: Discovery, perspective, and challenges in clinical use

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