Total fertilization failure: is it the end of the story?

Kahyaoğlu, Serkan; Demır, Berfu; Türkkanı, Ayten; Cınar, Ozgur; Dilbaz, Serdar; Dilbaz, Berna; Mollamahmutoğlu, Leyla

doi:10.1007/s10815-014-0281-5

Cited by 26 publications

(31 citation statements)

References 37 publications

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“…However, the investigators of this study were unable to determine a specific cut off value to predict fertilization failure [16]. In patients who have had a history of fertilization failure with ICSI and were undergoing a subsequent cycle, higher numbers of oocytes retrieved, mature oocytes and higher estradiol at trigger were associated with improved fertilization during the subsequent attempt [17]. In contrast to prior studies, our study examined the largest population over a 10-year period of time to establish thresholds for semen parameters on the day of oocyte retrieval which identify patients with an increased risk of low fertilization that may be clinically actionable for providers.…”

Section: Discussionmentioning

confidence: 83%

Semen parameters on the day of oocyte retrieval predict low fertilization during conventional insemination IVF cycles

Harris

Vanegas

Hariton

et al. 2018

J Assist Reprod Genet

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Purpose Poor fertilization during conventional IVF is difficult to predict in the absence of abnormal semen parameters; largescale studies are lacking. The purpose of this study is to evaluate factors associated with low fertilization rates in conventional insemination IVF cycles. Methods A retrospective cohort study evaluating demographic, reproductive evaluation, and IVF cycle characteristics to identify predictors of low fertilization (defined as 2PN/MII ≤ 30% per cycle). Participants were included if they were undergoing their first IVF cycle utilizing fresh autologous oocytes and conventional insemination with male partner's sperm (with normal pretreatment semen analysis). They were randomly divided into a training set and a validation set; validation modeling with logistic regression and binary distribution was utilized to identify covariates associated with low fertilization. Results Postprocessing sperm concentration of less than 40 million/ml and postprocessing sperm motility < 50% on the day of retrieval were the strongest predictors of low fertilization in the training dataset. Next, in the validation set, cycles with either low postprocessing concentration (≤ 40 million/ml) or low postprocessing progressive motility (≤ 50%) were 2.9-times (95% CI 1.4, 6.2) more likely to have low fertilization than cycles without either risk factor. Furthermore, cycles with low postprocessing concentration and progressive motility were 13.4 times (95% CI 4.01, 45.06) more likely to have low fertilization than cycles without either risk factor. Conclusions Postprocessing concentration and progressive motility on the day of oocyte retrieval are predictive of low fertilization in conventional IVF cycles with normal pretreatment diagnostic semen analysis parameters.

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Section: Discussionmentioning

confidence: 83%

Semen parameters on the day of oocyte retrieval predict low fertilization during conventional insemination IVF cycles

Harris

Vanegas

Hariton

et al. 2018

J Assist Reprod Genet

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“…Studies revealed 30% oocytes fail to fertilize in normal condition that may be due to sperm abnormalities and/or defects in oocyte activation factors (Swain & Pool, 2008;Kahyaoglu et al, 2014). Studies revealed 30% oocytes fail to fertilize in normal condition that may be due to sperm abnormalities and/or defects in oocyte activation factors (Swain & Pool, 2008;Kahyaoglu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In ART clinics, sperm selection during intracytoplasmic sperm injection (ICSI) technique is an important determinant for success in terms of fertilization rate. Studies revealed 30% oocytes fail to fertilize in normal condition that may be due to sperm abnormalities and/or defects in oocyte activation factors (Swain & Pool, 2008;Kahyaoglu et al, 2014). With respect to sperm parameters, properly condensed sperm nucleus is essential to undergo decondensation for pronucleus formation during fertilization (Tosti & Fortunato, 2011).…”

Section: Introductionmentioning

confidence: 99%

An illustration of human sperm morphology and their functional ability among different group of subfertile males

et al. 2018

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Condensed sperm chromatin is a prerequisite for natural fertilization. Some reports suggested the prevalence of chromatin condensation defects in teratozoospermia cases with head anomalies; conversely, earlier studies exemplified its occurrence in morphologically normal spermatozoa too. The aim of this study was to compare the condensation defects in correlation with head anomalies among different groups of subfertile males and its impact on the rate of fertilization in assisted reproduction procedures. Ultrastructure analysis of spermatozoa through scanning electron microscopy and atomic force microscopy could facilitate an in-depth evaluation of sperm morphology. Nuclear condensation defects (%) in spermatozoa were analyzed in 666 subjects, and its effect on the rate of fertilization was analyzed in 116 IVF and 90 intracytoplasmic sperm injection cases. There was no correlation of condensation defects with head anomalies (%). Student's t-test showed no significant changes in mean values of condensation defects in abnormal semen samples in comparison with the normal group. Condensation defects were observed in normal spermatozoa too, which was negatively associated with the rate of fertilization in IVF (p < 0.01), but intracytoplasmic sperm injection outcome remained unaffected. Ultrastructure study revealed sperm morphological features in height, amplitude, and three-dimensional views in atomic force microscopy images presenting surface topography, roughness property of head, and compact arrangement of mitochondria over axoneme with height profile at nanoscale. In pathological forms, surface roughness and nuclear thickness were marked higher than the normal spermatozoa. Thus, percentage of normal spermatozoa with condensation defects could be a predictive factor for the rate of fertilization in IVF. From diverse shapes of nucleus in AFM imaging, it could be predicted that defective nuclear shaping might be impeding the activity of some proteins/ biological motors, those regulate the proper Golgi spreading over peri-nuclear theca.

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“…Accordingly, we found a 4.8% rate of TFF in the IVF group, but no cases of TFF in the ICSI group. However, the rate of TFF was not significantly different from that of oocyte maturation in vivo, which has been found to be 5%-10% [13]. Interestingly, the sibling oocytes exhibiting TFF during IVF insemination displayed normal fertilization by ICSI because ICSI skips over the elaborate cascades of interactions between sperm and cumulus-oocyte complexes and deposits the sperm directly inside the ooplasm.…”

Section: Discussionmentioning

confidence: 88%

“…Interestingly, the sibling oocytes exhibiting TFF during IVF insemination displayed normal fertilization by ICSI because ICSI skips over the elaborate cascades of interactions between sperm and cumulus-oocyte complexes and deposits the sperm directly inside the ooplasm. In this context, ICSI may therefore offer more potential for the oocyte to fertilize and develop to the embryonic stage [13,14], even though ICSI itself cannot preclude TFF.…”

Section: Discussionmentioning

confidence: 99%

The effect of insemination methods on in vitro maturation outcomes

Pongsuthirak

2020

Clin Exp Reprod Med

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Objective: The aim of this study was to compare the effects of conventional insemination (in vitro fertilization [IVF]) and intracytoplasmic sperm injection (ICSI) on the fertilization, developmental competence, implantation potential, and clinical pregnancy rate of embryos derived from in vitro matured oocytes of patients with polycystic ovary syndrome (PCOS).Methods: A prospective study was carried out among 38 PCOS patients who had undergone in vitro maturation (IVM) treatment. In total, 828 immature oocytes were collected from 42 cycles and randomly assigned for insemination by IVF (416 oocytes) or ICSI (412 oocytes). After fertilization, the embryos were cultured until the blastocyst stage and single embryos were transferred after endometrial preparation and under ultrasound guidance.Results: No significant differences were found in the maturation rate (78.1% vs. 72.6% for IVF and ICSI insemination, respectively; p= 0.076), fertilization rate (59.4% vs. 66.9% for IVF and ICSI insemination, respectively; p= 0.063), or the formation of good-quality blastocysts (40.9% vs. 46.5% for IVF and ICSI insemination, respectively; p= 0.314). Implantation and clinical pregnancy also did not show significant differences.Conclusion: There was a comparable yield of in vitro matured oocytes derived from PCOS patients in terms of fertilization, blastocyst formation, implantation rate, and clinical pregnancy between IVF and ICSI insemination. These findings provide valuable insights for choosing assisted reproductive treatment in women with PCOS, as IVM offers promising outcomes and is less invasive and less costly.

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Total fertilization failure: is it the end of the story?

Abstract: Increasing the number of retrieved and mature oocytes may increase the success of fertilization in patients with a history of previous failed fertilization. However, live birth rate is still low in embryo transfer cycles.

Cited by 26 publications

References 37 publications

Semen parameters on the day of oocyte retrieval predict low fertilization during conventional insemination IVF cycles

Semen parameters on the day of oocyte retrieval predict low fertilization during conventional insemination IVF cycles

An illustration of human sperm morphology and their functional ability among different group of subfertile males

The effect of insemination methods on in vitro maturation outcomes

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