Total Bioaerosol Detection by Split Aptamer-Based Electrochemical Nanosensor Chips

Abstract

Bioaerosols could carry and spread harmful microorganisms, thus posing a continuous threat to human beings and livestock health. Early warning and management are crucial for controlling the spread of bioaerosols. Herein, we developed a split aptamer (SA)-based electrochemical nanosensor chip (denoted SAE-nChip) for rapid and sensitive detection of adenosine triphosphate (ATP) in bioaerosols. The platform features two components: split DNA aptamers for their ability to bind ATP and undergo target-induced assembly on the chip surface and ZIF-8@MXene composites for their ability to provide a high surface density of aptamer-binding sites and facilitate the electron transfer at the biointerface. The SAE-nChip was capable of detecting ATP with a detection limit of 10 pM. Furthermore, this assay allowed the detection of ATP in cultured microorganisms and collected real bioaerosols. Overall, this strategy of interfacing DNA aptamers with MXene-based composite materials represents a versatile approach for the ubiquitous detection of biochemical targets in bioaerosols.

On-site airborne pathogen detection for infection risk mitigation

“…A split aptamer (SA)-based electrochemical sensor chip was recently developed for the rapid detection of adenosine triphosphate in bioaerosols with a detection limit of 10 pM, which can be used for the ubiquitous detection of total bioaerosols. 265 Additionally, the transducing electrodes of electrochemical biosensors can be easily integrated onto different flexible or low-cost substrates (e.g., paper, polymers, and clothes). [266][267][268][269] For instance, the screen-printing method could enable the mass manufacturing of electronic pathogen sensing devices.…”

“…A split aptamer (SA)-based electrochemical sensor chip was recently developed for the rapid detection of adenosine triphosphate in bioaerosols with a detection limit of 10 pM, which can be used for the ubiquitous detection of total bioaerosols. 265 …”

CRISPR/Cas12a-Responsive Hydrogels for Conjugation-Free and Universal Indicator Release in Colorimetric Detection

“…However, most highly effective and commercial diagnostics still rely on advanced equipment using fluorescent readouts, which limits their use in low-cost, simple, and portable point-of-care testing (POCT) that is essential in resource-constrained environments and critical to preventing the spread of highly contagious diseases. In order to solve this issue, increasing efforts have been devoted to developing effective transduction strategies with more portable, cheaper, and/or easy-to-miniaturize readouts such as electrochemistry , and colorimetry. − …”