Total antioxidant capacity, catalase activity, and lipid peroxidation changes in seminal plasma of sex-reversed female and male rainbow trout (Oncorhynchus mykiss) during spawning season

İnanan, Burak Evren; Öğretmen, Fatih; Inanan, Tülden; Yılmaz, Feride Taşkın

doi:10.1016/j.theriogenology.2016.06.014

Cited by 15 publications

(13 citation statements)

References 59 publications

(2 reference statements)

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“…TBARS is an indicator of oxidative stress which causes a decline in function of spermatozoa (Dzyuba et al, ). TBARS content in SP changes throughout the spawning season and during in vitro storage of spermatozoa as well (İnanan, Öğretmen, İnanan, & Yılmaz, ; Lahnsteiner, Mansour, & Plaetzer, ; Shaliutina, Hulak, Gazo, Linhartova, & Linhart, ). Similar to pH, the current study has shown that TBARS content is an efficient marker for successful storage of not just for spermatozoa but also eggs.…”

Section: Discussionmentioning

confidence: 99%

Fertilization rate, motility, lipid peroxidation and pH changes after chilled storage of rainbow trout ( Oncorhynchus mykiss ) eggs and spermatozoa by a RMPI medium

İnanan

2019

Aquac Res

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The aim of this study was to evaluate the effects of Roswell Park Memorial Institute (RPMI) 1,640 medium on chilled storage of eggs and spermatozoa of rainbow trout (Oncorhynchus mykiss). After 3 days of storage, eggs in RPMI 1,640 media (pH 8.2, 9 and 10), Cortland medium and coelomic fluid were inseminated with fresh spermatozoa (Experiment 1). Eggs in RPMI 1,640 medium at pH 8.2 shown the lowest thiobarbituric acid‐reactive substances (TBARS, 0.053 ± 0.003 nmol/ml) and pH changes (from 8.20 ± 0.01 to 8.18 ± 0.01), the highest fertilization rate (82 ± 3%). Undiluted and diluted spermatozoa at ratios of 1:2 and 1:9 with RPMI 1,640 media (pH 8.2, 9 and 10) and Cortland medium were inseminated with fresh eggs (Experiment 2). Spermatozoa in RPMI 1,640 medium at pH 9 (1:9) caused the lowest TBARS content (0.037 ± 0.002 nmol/ml) and pH changes (from 9.00 ± 0.01 to 8.98 ± 0.01), the highest fertilization rate (77 ± 2%) and motility parameters. Based on Experiments 1 and 2, eggs and spermatozoa were stored for another 3 days in RPMI 1,640 medium at pH 8.2 and 9 (1:9) respectively (Experiment 3). Fertilization rate of storage eggs and spermatozoa in Experiment 3 was 79 ± 5%, showing successfully storage of rainbow trout gametes with the same medium.

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Section: Discussionmentioning

confidence: 99%

Fertilization rate, motility, lipid peroxidation and pH changes after chilled storage of rainbow trout ( Oncorhynchus mykiss ) eggs and spermatozoa by a RMPI medium

İnanan

2019

Aquac Res

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“…High APA values can be related to the greater protection of immature spermatozoa against enzymatic proteolysis in SRF compared to that of normal spermatozoa. The protein concentrations in SRF seminal plasma are generally within the range of 2.9 ± 1.1-8.2 ± 1.3 mg mL −1 and are significantly higher in SRFs than in normal males (Table 4; [49,54,56,91]). Moreover, these values are higher in the testicular semen of normal males than in that of SRFs [49].…”

Section: Biochemical Characteristics 431 Antitrypsin Activity and Protein Concentrationmentioning

confidence: 99%

Characteristics and Cryopreservation of Semen of Sex-Reversed Females of Salmonid Fish

Judycka

Nynca

Hliwa

et al. 2021

IJMS

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Sex reversal has been used as a breeding strategy by salmonid fish to produce genetically and phenotypically single sex populations. Production of all-female fish has great importance for the creation of monosex female triploids of salmonid fish, which are valued for their sterility, lack of female maturation, and larger commercial size. Among salmonids, the majority of rainbow trout (Oncorhynchus mykiss) production is based on all-female production with a high proportion of all-female triploid production in Europe. The main aim of this review is to present the recent knowledge regarding sex-reversed females (SRFs) of salmonid fish. We discuss the methods of sex reversal as well as their effects on the morphology and histology of the reproductive tract. We focus on the characteristics of SRF semen as well as the factors determining semen quality. The lower quality of SRF sperm compared to that of normal males has resulted in the need for the artificial maturation of semen. Most importantly, methods of semen storage—both short-term and long-term (cryopreservation)—that can improve hatchery operations are presented with the special emphasis on recent progress in development of efficient cryopreservation procedures and use of cryopreserved semen in hatchery practice. Moreover, we also address the emerging knowledge concerning the proteomic investigations of salmonid sperm, focusing primarily on the proteomic comparison of normal male and SRF testicular semen and presenting changes in SRF rainbow trout sperm proteome after in vitro incubation in artificial seminal plasma.

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“…Eurasian perch (Perca fluviatilis) in particular presented a TAC value 200 times higher than that of rainbow trout (Słowińska et al 2013). Some authors agree that the antioxidant capacity may be influenced by factors such as habitat, physiological state, nutritional condition (Słowińska et al 2013;Atli et al 2016;Klein et al 2017) and spawning season; in the case of trout, it could depend on whether the sperm is from normal male or sex-reversed individuals (İnanan et al 2016;İnanan & Yılmaz 2018;Judycka et al, 2020). Furthermore, a recent finding suggests that the antioxidant capacity in semen of common carp depends on the seminal fraction (Shaliutina-Kolesova et al, 2019).…”

Section: Antioxidant Defence System In Fish Semenmentioning

confidence: 99%

“…2017) and spawning season; in the case of trout, it could depend on whether the sperm is from normal male or sex‐reversed individuals (İnanan et al . 2016; İnanan & Yılmaz 2018; Judycka et al, 2020). Furthermore, a recent finding suggests that the antioxidant capacity in semen of common carp depends on the seminal fraction (Shaliutina‐Kolesova et al, 2019).…”

Section: Antioxidant Defence System In Fish Semenmentioning

confidence: 99%

Oxidative stress and use of antioxidants in fish semen cryopreservation

Sandoval‐Vargas

Jiménez

González

et al. 2020

Reviews in Aquaculture

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Reactive oxygen species (ROS) have been proposed as one of the main causes of the impairment of fish spermatozoa integrity and functionality during cryopreservation. The high content of unsaturated fatty acids in sperm cells and the low antioxidant capacity of diluted semen are key factors in making sperm cells susceptible to ROS attacks. For this reason, some recent studies have determined the antioxidant status of the seminal plasma and spermatozoa of fish species. Additionally, some studies have evaluated the effects of antioxidants on post-thaw sperm quality. Although ROS are certainly involved in sperm damage, other factors, such as ice crystal formation, seem to play a crucial role in cryodamage. This challenge has not yet been resolved because both the endogenous antioxidant capacity of the semen and its response to different supplementation practices seem to present specific inter-and intraspecies characteristics and effects. This review summarises knowledge on antioxidant defence and oxidative stress in fish semen, as well as antioxidant supplementation in cryopreservation media, in order to establish perspectives for future studies.

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Total antioxidant capacity, catalase activity, and lipid peroxidation changes in seminal plasma of sex-reversed female and male rainbow trout (Oncorhynchus mykiss) during spawning season

Cited by 15 publications

References 59 publications

Fertilization rate, motility, lipid peroxidation and pH changes after chilled storage of rainbow trout ( Oncorhynchus mykiss ) eggs and spermatozoa by a RMPI medium

Fertilization rate, motility, lipid peroxidation and pH changes after chilled storage of rainbow trout ( Oncorhynchus mykiss ) eggs and spermatozoa by a RMPI medium

Characteristics and Cryopreservation of Semen of Sex-Reversed Females of Salmonid Fish

Oxidative stress and use of antioxidants in fish semen cryopreservation

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