Total antioxidant capacity assay of human serum using copper(II)-neocuproine as chromogenic oxidant: The CUPRAC method

Apak, Reşat; Güçlü, Kubilay; Özyürek, Mustafa; Karademir, Saliha Esin; Altun, Mehmet

doi:10.1080/10715760500210145

Cited by 263 publications

(217 citation statements)

References 23 publications

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“…The degree of colour change (either an increase or decrease of absorbance at a given wavelength) is correlated to the concentration of antioxidants in the sample. ABTS/TEAC (Trolox ® -equivalent antioxidant capacity) [73,74] and DPPH [75][76][77] are decolorization assays, whereas in Folin total phenols assay [78,79], FRAP (ferric reducing antioxidant power) [80,81] and CUPRAC (cupric reducing antioxidant capacity) [82,83], there is an increase in absorbance at a prespecified wavelength as the antioxidant reacts with the chromogenic reagent (i.e., in the latter two methods, the lower valencies of iron and copper, namely Fe(II) and Cu(I), form charge-transfer complexes with the ligands, respectively). The basic chromophores used in Folin, ABTS/TEAC, FRAP, ferricyanide, ferric-phenanthroline, DPPH and CUPRAC assays are shown Figure 3.…”

Section: Total Antioxidant Capacity (Tac) Assays Applied To Phenolicsmentioning

confidence: 99%

Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay

et al. 2007

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It would be desirable to establish and standardize methods that can measure the total antioxidant capacity level directly from vegetable extracts containing phenolics. Antioxidant capacity assays may be broadly classified as electron transfer (ET)− and hydrogen atom transfer (HAT)−based assays. The majority of HAT assays are kineticsbased, and involve a competitive reaction scheme in which antioxidant and substrate compete for peroxyl radicals thermally generated through the decomposition of azo compounds. ET−based assays measure the capacity of an antioxidant in the reduction of an oxidant, which changes colour when reduced. ET assays include the ABTS/TEAC, CUPRAC, DPPH, Folin-Ciocalteu and FRAP methods, each using different chromogenic redox reagents with different standard potentials. This review intends to offer a critical evaluation of existing antioxidant assays applied to phenolics, and reports the development by our research group of a simple and low-cost antioxidant capacity assay for dietary polyphenols, vitamins C and E, and human serum antioxidants, utilizing the copper(II)-neocuproine reagent as the chromogenic oxidizing agent, which we haved named the CUPRAC (cupric ion reducing antioxidant capacity) method. This method offers distinct advantages over other ET−based assays, namely the selection of working pH at Molecules 2007, 12 1497 physiological pH (as opposed to the Folin and FRAP methods, which work at alkaline and acidic pHs, respectively), applicability to both hydrophilic and lipophilic antioxidants (unlike Folin and DPPH), completion of the redox reactions for most common flavonoids (unlike FRAP), selective oxidation of antioxidant compounds without affecting sugars and citric acid commonly contained in foodstuffs and the capability to assay -SH bearing antioxidants (unlike FRAP). Other similar ET-based antioxidant assays that we have developed or modified for phenolics are the Fe(III)− and Ce(IV)−reducing capacity methods.

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Section: Total Antioxidant Capacity (Tac) Assays Applied To Phenolicsmentioning

confidence: 99%

Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay

et al. 2007

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“…Because of the high cost and relatively unstable radical chromogen reagents such as ABTS and DPPH, an easily adaptable and low-cost method known as cupric ion reducing antioxidant capacity (CUPRAC) was developed for total antioxidant assay in food stuff and human plasma (Apak et al, 2004(Apak et al, , 2005. Recently, an indirect spectrophotometric method that is more accurate, simple, low-cost, sensitive and inexpensive has also been developed for the determination of total antioxidant capacity of simple antioxidant compounds of several plants (Ozyurt et al, 2007).…”

Section: Article In Press Ia Muraina Et Al / Phytomedicine ] (]]]]mentioning

confidence: 99%

Can MTT be used to quantify the antioxidant activity of plant extracts?

2009

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Antioxidant properties of three different medicinal plant extracts from northern Nigeria were evaluated using the traditional qualitative 2,2, diphenyl-picrylhydrazyl (DPPH) method and a new 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) microdilution method described here. The results were in agreement. It is a simple, rapid and inexpensive method compared with many other methods for determining quantitative antioxidant activity of plant extracts.

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“…In this method, Cu 2+ is converted to Cu + by both small molecules and protein. The reduced ion is chelated with a colorimetric probe giving a broad absorbance peak around 450 nm, proportional to the TAC (29).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Microbiota and oxidant-antioxidant balance in systemic lupus erythematosus

et al. 2017

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ResumenBackground: Systemic lupus erythematosus (SLE) is a chronic infl ammatory disease of autoimmune nature, in which oxidative stress is implicated. Aim: Compare the concentrations of dietary and blood antioxidants, as well as gut microbiota, with serum malondialdehyde (MDA) and C reactive protein (CRP) in 21 subjects suffering from non-active systemic lupus erythematosus (SLE) and 21 age and gender-matched controls. Methods: General biochemical parameters and CRP were determined by enzymatic methods: copper, zinc and selenium by inductively coupled plasma mass spectrometry (ICP-MS), MDA and total antioxidant capacity (TAC) by spectrophotometric methods, gut microbiota by metagenomic analyses and dietary intake by means of food frequency questionnaire. Results: No signifi cant differences were found in diet between lupus patients and the control group, with the exception of trans fatty acids intake, which was higher in patients. In addition, higher concentration of serum copper and lower of zinc in SLE were found. Serum copper was positively associated with CRP and also, this protein with the proportion of Lentisphaerae, Proteobacteria and Verrucomicrobia in feces. Moreover, MDA levels displayed inverse correlations with the Cyanobacteria and Firmicutes groups, while Actinobacteria showed a positive association. The lupus subjects with presence of anti-SSA/Ro were related to higher levels of serum zinc. Conclusion: These results could be useful in the future to go deeper into the understanding of this complex disease. AbstractIntroducción: el lupus eritematoso sistémico es una enfermedad infl amatoria crónica en la que está implicado el estrés oxidativo. Objetivo: evaluar la concentración de antioxidantes de la dieta y sanguíneos, así como de la microbiota sobre las concentraciones de malondialdehído y proteína C reactiva en 21 pacientes de lupus y 21 controles pareados por edad y sexo. Métodos: los parámetros bioquímicos de rutina y proteína C reactiva se determinaron a través de métodos enzimáticos: cobre, zinc y selenio por espectrometría de masas, malondialdehído y capacidad antioxidante total por métodos espectrofotométricos, la microbiota fecal por técnicas metagenómicas y la dieta a través de cuestionarios de frecuencia de consumo. Resultados: no se han observado diferencias en la dieta en los pacientes con lupus respecto al grupo control, excepto en la ingesta de ácidos grasos trans, siendo mayor en el grupo de lupus. En estas pacientes se observaron mayores niveles circulantes de cobre y menores de zinc. La concentración de cobre en suero se relacionó directamente con los niveles de proteína C reactiva y esta proteína, a su vez, con la proporción de Lentisphaerae, Proteobacteria y Verrucomicrobia en heces. Además, mientras que los niveles de malondialdehído se asociaban inversamente con la proporción de Cyanobacteria y Firmicutes, con Actinobacteria se encontró una correlación positiva. La presencia de anti-SSA/Ro en lúpicas se relaciona con mayores concentraciones de zinc. Conclusió...

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Total antioxidant capacity assay of human serum using copper(II)-neocuproine as chromogenic oxidant: The CUPRAC method

Cited by 263 publications

References 23 publications

Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay

Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay

Can MTT be used to quantify the antioxidant activity of plant extracts?

Microbiota and oxidant-antioxidant balance in systemic lupus erythematosus

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