Total and isotype humoral responses in cattle vaccinated with foot and mouth disease virus (FMDV) immunogen produced either in bovine tongue tissue or in BHK-21 cell suspension cultures

Capozzo, Alejandra Victoria; Periolo, Osvaldo; Robiolo, Blanca; Seki, Cristina; Torre, J.L. La; Grigera, Pablo R.

doi:10.1016/s0264-410x(96)00284-8

Cited by 45 publications

(36 citation statements)

References 17 publications

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“…On the one hand, this indicates that at least in some of the animals, circulating antibodies might have found virulent virus administered by the oronasal route. On the other hand, these observations are concurrent with previous findings by our group and others regarding the potential in vivo FMDV-neutralizing roles for the bovine IgM in naive cattle at early times postinfection (22) and the IgG1 isotype in vaccinated animals at later times postimmunization (24,28). Most significantly, we described for the first time that, besides the systemic and ASC responses in the draining lymph node, intramuscular (systemic) FMD vaccination in the neck also was capable of promoting local virus-specific ASC in respiratory lymphoid tissues in cattle.…”

Section: Discussionsupporting

confidence: 91%

Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection

Pega

Giacomo

Bucafusco

et al. 2015

J Virol

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Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied the generation of antibody responses in local lymphoid tissues along the respiratory system in vaccinated and further aerosol-infected cattle. Animals immunized with a high-payload monovalent FMD vaccine developed high titers of neutralizing antibodies at 7 days postvaccination (dpv), reaching a plateau at 29 dpv. FMDV-specific antibody-secreting cells (ASC), predominantly IgM, were evident at 7 dpv in the prescapular lymph node (LN) draining the vaccination site and in distal LN draining the respiratory mucosa, although in lower numbers. At 29 dpv, a significant switch to IgG1 was clear in prescapular LN, while FMDV-specific ASC were detected in all lymphoid tissues draining the respiratory tract, mostly as IgM-secreting cells. None of the animals (n ‫؍‬ 10) exhibited FMD symptoms after oronasal challenge at 30 dpv. Three days postinfection, a large increase in ASC numbers and rapid isotype switches to IgG1 were observed, particularly in LN-draining virus replication sites already described. These results indicate for the first time that systemic FMD vaccination in cattle effectively promotes the presence of anti-FMDV ASC in lymphoid tissues associated with the respiratory system. Oronasal infection triggered an immune reaction compatible with a local anamnestic response upon contact with the replicating FMDV, suggesting that FMD vaccination induces the circulation of virusspecific B lymphocytes, including memory B cells that differentiate into ASC soon after contact with the infective virus. F oot-and-mouth disease (FMD) is a highly contagious and acute viral disease affecting a wide range of economically important livestock species (1). All domestic biungulates are susceptible to infection with the FMD virus (FMDV); in addition, a number of wildlife species may act as reservoirs for the virus under particular ecological conditions (2). Lethality has been described for young animals and certain FMDV strains (3). However, the main disruptive potential of this transboundary disease is the high morbidity rate and the numerous indirect losses associated with its incursions into territories with susceptible populations. Consequently, FMD outbreaks may result in severe and far-reaching economic losses due to the interruption of regional and international trade in developed countries (4, 5), but also importantly, due to the loss of animals, production efficiency, and genetic diversity in developing regions (6).Cattle are highly susceptible to FMDV, and virus usually gains entry through the respiratory tract of the animals (3). The soft palate and pharynx were identified as primary sites of FMDV replication and persistence in bovines infected through the oronasal route (7,8). FMDV infection progresses through replication in pneumocytes, permitting the virus to accomplish an extensi...

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Section: Discussionsupporting

confidence: 91%

Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection

Pega

Giacomo

Bucafusco

et al. 2015

J Virol

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“…FMDV-specific isotype ELISA was performed as reported by Capozzo et al (30), except that sheep anti-bovine IgG1 and IgG2 HRP-conjugated antibodies were used at a dilution of 1:750, and sheep anti-bovine IgM HRP-conjugated antibody was used at 1:5,000 (BD-Serotec, Oxford, United Kingdom).…”

Section: Methodsmentioning

confidence: 99%

Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle

Pega

Bucafusco

Giacomo

et al. 2013

J Virol

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Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.

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“…The response is directed to epitopes on the three external structural proteins (see "Antigenic variation" above), and good protective immunity is apparent between 7 and 14 days after either infection or vaccination. In cattle, the immunoglobulin G1 (IgG1) response predominates over IgG2 (86,326,417), and antibody, including IgA, can be detected in upper respiratory secretions early in infection (415,417). The neutralization of virus within the host may occur by mechanisms similar to those occurring in in vitro neutralization; however, there is a suggestion that macrophages may play a role in clearing the virus from the infected animal by phagocytosis of opsonized virus (306,307,383).…”

Section: Host Responsementioning

confidence: 99%

Foot-and-Mouth Disease

2004

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Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. The disease was initially described in the 16th century and was the first animal pathogen identified as a virus. Recent FMD outbreaks in developed countries and their significant economic impact have increased the concern of governments worldwide. This review describes the reemergence of FMD in developed countries that had been disease free for many years and the effect that this has had on disease control strategies. The etiologic agent, FMD virus (FMDV), a member of the Picornaviridae family, is examined in detail at the genetic, structural, and biochemical levels and in terms of its antigenic diversity. The virus replication cycle, including virus-receptor interactions as well as unique aspects of virus translation and shutoff of host macromolecular synthesis, is discussed. This information has been the basis for the development of improved protocols to rapidly identify disease outbreaks, to differentiate vaccinated from infected animals, and to begin to identify and test novel vaccine candidates. Furthermore, this knowledge, coupled with the ability to manipulate FMDV genomes at the molecular level, has provided the framework for examination of disease pathogenesis and the development of a more complete understanding of the virus and host factors involved

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Total and isotype humoral responses in cattle vaccinated with foot and mouth disease virus (FMDV) immunogen produced either in bovine tongue tissue or in BHK-21 cell suspension cultures

Cited by 45 publications

References 17 publications

Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection

Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection

Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle

Foot-and-Mouth Disease

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