Total and Toxocara canis larval excretory/secretory antigen‐ and allergen‐specific IgE in atopic and non‐atopic dogs

Self Cite

Section: Discussionmentioning

confidence: 82%

“…Total IgE was measured using a commercial canine IgE ELISA quantitation kit (Bethyl Laboratories; Montgomery, AL, USA) and results were expressed in ng/mL. All samples were analysed in duplicate using a previously reported assay . Another study permitted the determination of the range of total IgE levels in normal dogs …”

Section: Methodsmentioning

confidence: 99%

A pilot study of total and allergen‐specific IgE serum levels during anestrous, estrous and pregnancy in healthy female dogs

Tarpataki

Thuróczy

Fischer

et al. 2018

Self Cite

“…By contrast, adult allergic dogs exclusively showed elevated Treg cell levels in the peripheral blood 18‐20 . Elevated Tregs also were demonstrated in an experimental model of canine AD 21 …”

Section: Introductionmentioning

confidence: 92%

Atopic dermatitis in West Highland white terriers – Part III: early life peripheral blood regulatory T cells are reduced in atopic dermatitis

Rostaher

Rodriguez‐Campos

Deplazes

et al. 2021

Self Cite

Background -Regulatory T (Treg) cells are involved in homeostasis of immune regulation and suppression of inflammation and T-cell polarisation. Current knowledge regarding the role of Treg cells in the initiation of allergic disease is limited for both people and dogs.Objectives -To explore the role of circulating Treg cells and their possible influencing factors, on the development of atopic dermatitis (AD).Methods and materials -This study followed part of a birth cohort of West Highland white terrier dogs and classified them according to eventual clinical signs of AD (i.e. allergic versus healthy). The Treg phenotypes were assessed longitudinally by flow cytometry at 3, 3-12 and 12-36 months of age, and associated with development of AD. Different early life antigenic factors [endotoxins and allergens in house dust, Toxocara canis-specific immunoglobulin (Ig)E/IgG, allergen-specific and total IgE, skin microbiota] were measured at three months of age, and a possible association with Treg cell levels was assessed.Results -The percentages of CD4 + CD25 + Foxp3 + Treg cells in healthy dogs were significantly higher at in 3month-old (mean 4.5% healthy versus 3.3% allergic; P = 0.021) and <1-year-old (4.0% healthy versus 2.9% allergic; P = 0.028) dogs when compared to percentages of Treg cells in dogs that developed AD. There was a significantly positive correlation between the relative abundance of Lachnospiraceae on the skin and CD4 + CD25 + Foxp3 + Treg cells in puppies that became allergic (r = 0.568, P = 0.017). Conclusion and clinical importance -Further large-scale studies are needed to identify the practical value of these findings in AD diagnosis, treatment and prevention.

“…1,8 Canine atopic-like dermatitis (ALD) has been defined as an inflammatory and pruritic skin disease with clinical features identical to those seen in canine AD, in which an IgE response to environmental or other allergens cannot be documented. 9,10 Because total serum IgE levels have a poor correlation with the atopic status in dogs, [11][12][13] this parameter cannot be used to distinguish AD and ALD in dogs. Thus, in dogs, the differentiation has been made on the basis of the results of intradermal (IDT) and serum allergen-specific IgE tests (AST).…”

Section: Introductionmentioning

confidence: 99%

Comparison of demographic data, disease severity and response to treatment, between dogs with atopic dermatitis and atopic‐like dermatitis: a retrospective study

Botoni

Torres

Koch

et al. 2018

Background Limited information is available describing the features of canine atopic‐like dermatitis (ALD) compared with atopic dermatitis (AD). Objectives To compare demographic data, disease severity and response to therapy between ALD and AD dogs. Animals Two hundred and fifty‐three atopic dogs with intradermal and serum allergen‐specific IgE test results were selected retrospectively. Methods and materials Dogs were enrolled into the ALD group if both IgE tests were negative and into the AD group if at least one test was positive. Demographic data, pruritus level and number of body sites affected before and during therapy, in addition to maintenance therapy protocols, were compared between groups. Results There were 216 (85.38%) dogs in the AD group and 37 (14.62%) in the ALD group. The soft‐coated wheaten terrier, American Staffordshire terrier, English bulldog and Labrador retriever were over‐represented in the AD group. No significant differences between the groups were noted regarding the other demographic variables evaluated. There were no differences in the mean pruritus scores and number of affected body sites at the first visit or during treatment. Furthermore, no significant differences between the groups were noted for the maintenance treatment scores and reduction of pruritus level and number of body sites affected during treatment. Conclusions and clinical significance The soft‐coated wheaten terrier, American Staffordshire terrier, English bulldog and Labrador retriever were over‐represented in the AD group. No significant differences in the other demographic data and clinical features were noted between dogs with ALD and AD in the present study.