Total Amino Acids by UHPLC–UV in Infant Formulas and Adult Nutritionals, First Action 2018.06

Jaudzems, Greg; Guthrie, Joseph; Lahrichi, S.; Fuerer, Christophe

doi:10.5740/jaoacint.19-0036

Cited by 18 publications

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“…The SPIFAN Nutrients Expert Review Panel recommended the exclusion of taurine from the final method because other methods such as AOAC Method 997.05 ( 11 ) can measure low levels of taurine with higher accuracy. Taurine results are included in this manuscript for the sake of completeness, and readers can refer to the single-laboratory validation manuscript ( 7 ) for the taurine protocol.…”

Section: Resultsmentioning

confidence: 99%

“…A faster method combining cysteine/cystine conversion by 3,3’-dithiodipropionic acid (DDP) into S-2-carboxyethylthiocysteine (XCys) during the acid hydrolysis step ( 5 ) and pre-column derivatization with 6-aminoquinolyl- N -hydroxysuccinimidyl carbamate (AQC) followed by reversed-phase ultra-HPLC (UHPLC) separation ( 6 ) was developed for the quantification of total amino acids and taurine (with the exception of tryptophan) in infant formulas and adult nutritionals ( 7 ). This method was granted First Action status by AOAC SPIFAN in September 2018 (AOAC Method 2018.06 ), and a collaborative study was set up to provide additional method performance data.…”

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confidence: 99%

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Determination of Total Amino Acids in Infant Formulas, Adult Nutritionals, Dairy, and Cereal Matrixes by UHPLC–UV: Interlaboratory Validation Study, Final Action 2018.06

Jaudzems¹,

Fuerer

2022

Journal of AOAC INTERNATIONAL

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Background A method for the quantification of total amino acids (including taurine and excluding tryptophan) using ultra- HPLC separation coupled to UV detection (UHPLC–UV) was granted First Action status (AOAC 2018.06) by the AOAC INTERNATIONAL Stakeholder Program for Infant Formula and Adult Nutritionals (SPIFAN) in 2018. Objective An interlaboratory study was conducted to further assess method performance against the AOAC Standard Method Performance Requirements (AOAC SMPR® 2014.013). Dairy and cereal matrixes were added to expand the scope of the method in collaboration with IDF (International Dairy Federation), ISO (International Organization for Standardization), and AACCI (American Association of Cereal Chemists International, now Cereals & Grains Association). Methods Sixteen different matrixes were chosen to cover the requirements of AOAC, IDF/ISO, and AACCI. Blind duplicate samples were organized into specific series to ensure that each pair was analyzed on the same day. Fifteen laboratories returned results. Data from four laboratories were considered invalid and removed from the dataset. Remaining data were assessed according to the Appendix D of the AOAC Official Methods of AnalysisSM (guidelines for collaborative study procedures). Results This method generally met the requirements listed in the SMPR for infant formulas and adult nutritionals, except for taurine. Method performance was comparable in dairy and cereal matrixes. Five different UHPLC instruments were used with either commercial or in-house reagents, demonstrating that the method is not limited to a single supplier. Conclusion This method was recommended for Final Action in infant and adult/pediatric nutritional formulas by the AOAC SPIFAN Nutrients Expert Review Panel in April 2021, with the exception of taurine. The corresponding IDF/ISO Draft International Standard (DIS) was approved by national bodies in May 2022, and comments collected during the ballot were incorporated into this manuscript. Highlights AOAC Official Method 2018.06 for the determination of total amino acids in infant formulas, adult nutritionals, dairy, and cereal matrixes was successfully validated in an interlaboratory study.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Determination of Total Amino Acids in Infant Formulas, Adult Nutritionals, Dairy, and Cereal Matrixes by UHPLC–UV: Interlaboratory Validation Study, Final Action 2018.06

Jaudzems¹,

Fuerer

2022

Journal of AOAC INTERNATIONAL

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“…For this purpose, 50 μL of supernatant was transferred into chromatographic vials, evaporated by nitrogen gas and redissolved in hydrolysis buffer solution (260 μL of water, 120 μL of 0.1% dithiodipropionic acid/ 0.2 M sodium hydroxide, 120 μL of 0.2 M hydrochloric acid, 500 μL of 37% hydrochloric acid). Before closing, vials were flushed with nitrogen for 5 s, vortexed and incubated at a temperature of 110 °C for 15 h [ 88 ]. In vitro protein digestibility was assessed according to the OPA method [ 89 ], with minor modifications.…”

Section: Methodsmentioning

confidence: 99%

Hydrolysis of Soybean Milk Protein by Papain: Antioxidant, Anti-Angiotensin, Antigenic and Digestibility Perspectives

et al. 2022

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The objective of the investigation was to understand the biochemical activities of hydrolysate of soybean milk protein (SMP). Hydrolysis was carried out by different concentrations of papain (0.008 g·L−1, 0.016 g·L−1, 0.032 g·L−1 and 0.064 g·L−1). The antioxidant capacity was measured by the ferric-reducing ability of plasma (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays. The anti-angiotensin activity of hydrolysate was measured by the recombinant angiotensin converting enzyme and substrate Abz-FRK(Dnp)-P. The contributions of the Kunitz trypsin inhibitor (KTI) and Bowman–Birk inhibitor (BBI) on antigenicity, and the in vitro digestion of papain-hydrolyzed SMP were studied. Rabbit polyclonal anti-KTI and anti-BBI antibodies together with peroxidase-labelled goat anti-Rb IgG secondary antibody were used to identify the antigenicity of KTI and BBI in unhydrolyzed and papain-hydrolyzed SMP. The antioxidant capacity and anti-angiotensin activity of SMP were increased after the papain hydrolysis of SMP. The KTI- and BBI-specific antigenicity were reduced in SMP by increasing the concentration of papain. However, there was interaction between papain-hydrolyzed SMP and trypsin in native gel, while interaction with chymotrypsin was absent. The interaction between trypsin and SMP was reduced due to the hydrolysis of papain in a concentration-dependent manner. According to the in vitro gastrointestinal digestion simulation protocol (Infogest), the digestibility of SMP was not statistically increased.

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“…For quantify amines and amino acids in human disease phenotyping 65 . d) UHPLC-UV was applied for the analysis of total amino acid in infant formulas and adult nutritionals 66 .…”

Section: Examples: A)mentioning

confidence: 99%

A Mini-review on Ultra Performance Liquid Chromatography

et al. 2021

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Chromatography is a widely used analytical tool for separating a mixture of compounds into individual component. High performance liquid chromatography (HPLC) is one of the most important methods used for the separation, identification and quantification of a compounds present in a mixture. It meets many criteria of analysis but its main drawbacks are it is relatively time consuming to run a chromatogram and consumes high amount of solvent compared to other analytical methods. There is a need to develop a method which can overcome these drawbacks of HPLC. Ultra performance liquid chromatography (UPLC) is the new approach which opens novel direction in the field of liquid chromatography. It works on similar principle but shows better performance than conventional HPLC. UPLC is a technique of liquid chromatography with improved runtime and sensitivity with less than 2 μm particle size. The UPLC separation process is carried out under very high pressure (up to100 MPa). Additionally, it reduces the cost of reagent with shorter run time as compared to conventional HPLC. This article updated until 2020, provides a general review on the principle, instrumentation and application of UPLC in different fields of science.

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Total Amino Acids by UHPLC–UV in Infant Formulas and Adult Nutritionals, First Action 2018.06

Cited by 18 publications

References 0 publications

Determination of Total Amino Acids in Infant Formulas, Adult Nutritionals, Dairy, and Cereal Matrixes by UHPLC–UV: Interlaboratory Validation Study, Final Action 2018.06

Determination of Total Amino Acids in Infant Formulas, Adult Nutritionals, Dairy, and Cereal Matrixes by UHPLC–UV: Interlaboratory Validation Study, Final Action 2018.06

Hydrolysis of Soybean Milk Protein by Papain: Antioxidant, Anti-Angiotensin, Antigenic and Digestibility Perspectives

A Mini-review on Ultra Performance Liquid Chromatography

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