Early embryonic development arrest (EEDA) is a unique
form of early
spontaneous abortion in pregnant women, which is previously suggested
to be associated with metabolic abnormalities. Noninvasive biomarkers
would significantly improve its diagnosis and clinical outcome. Here,
we performed a targeted metabolomics study in plasma from EEDA patients
(n = 27) and normal pregnant women (NPW, n = 27) using liquid chromatography coupled with mass spectrometry
(LC–MS) to identify potential diagnostic marker metabolites.
Our results showed significantly different plasma metabolic profiles
between EEDA patients and NPW. Particularly, EEDA patients showed
significant alterations in amino acid, carbohydrate, and vitamin metabolism,
which were characterized by 21 significantly increased metabolites
and five decreased metabolites in plasma. Further receiver operating
characteristic analysis showed that an optimal combination of S-methyl-5′-thioadenosine, kynurenine, leucine, and
malate could be used as a panel of metabolites for EEDA diagnosis.
The area under the curve of the metabolite panel was 0.941, suggesting
a better performance than any single metabolite for the diagnosis
of EEDA. In summary, our study identifies a panel of differential
metabolites in plasma that could act as potential biomarkers for the
diagnosis of EEDA in clinical settings.