Torque teno virus (TTV) in immunosuppressed host: Performances studies of TTV R-Gene® kit and donors and recipients kidney samples genotyping

Kulifaj, Dorian; Essig, Marie; Meynier, F.; Pichon, Nicolas; E, Munteanu; Moulinas, Rémi; Joannes, Martine; Heckel, David G.; Combrissson, J.; Barranger, C.; Alain, Sophie

doi:10.1016/j.jcv.2016.08.206

Cited by 2 publications

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“…TTV viral DNA (elution volume 50 μL) was extracted from 200 μL of plasma sample using an easyMag extractor (bioMérieux, Marcy-l’Etoile, France) following the manufacturer’s instructions. The presence and viral load of TTV were then determined using the TTV R-GENE ® kit (available for research use only, not for diagnostic, Ref#69-030; bioMérieux, Marcy-l’Etoile, France) as previously described [ 19 , 20 ]. Log of TTV DNA copy number/mL (Log copies/mL) plasma are used to describe TTV viral load.…”

Section: Methodsmentioning

confidence: 99%

Torque Teno Virus Viral Load as a Marker of Immune Function in Allogeneic Haematopoietic Stem Cell Transplantation Recipients

Mouton

Conrad

Bal

et al. 2020

Viruses

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Torque teno virus (TTV) has been proposed as a surrogate biomarker of T-cell function in allogeneic–haematopoietic–stem-cell transplantation (allo-HSCT). Conflicting data exists regarding the value of TTV to assess the degree of immunosuppression. The aim of the present study was to investigate the correlation between TTV viral load and immune function. Using samples from a prospective cohort composed of healthy-volunteers (HV) and allo-HSCT recipients at 6 months post-transplantation, we assessed the correlation between TTV viraemia and immune cell counts or T-cell proliferation capacity post-phytohaemagglutinin stimulation. TTV viraemia was detected in 68% of HV (n = 80) and 100% of allo-HSCT recipients (n = 41; p < 0.001); it was significantly higher in allo-HSCT recipients (3.9 vs. 2.1 Log copies/mL, p < 0.001). There was no correlation between T-cell function and CD3+T-cell count (rho: 0.002) suggesting that T-cell count can normalise without full functional recovery. Furthermore, no significant correlation was observed between TTV viraemia and absolute total/subset lymphocyte counts (rho: <0.13). The highest correlation was observed between TTV viral load and T-cell proliferation capacity (rho: −0.39). We therefore report an inverse correlation between T-cell function and TTV viraemia that is independent of T-cell count. Monitoring of TTV viraemia could be a fast suitable option to objectively assess the competence of immune function in at-risk populations.

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Section: Methodsmentioning

confidence: 99%

Torque Teno Virus Viral Load as a Marker of Immune Function in Allogeneic Haematopoietic Stem Cell Transplantation Recipients

Mouton

Conrad

Bal

et al. 2020

Viruses

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“…In recent years, TTV has been studied by various research groups as a potential marker of immunosuppression following transplantation. TTV levels have been shown to increase at the start of antirejection treatment, reaching a relative plateau phase between 3 and 6 months after transplantation [12]. It is currently thought that an ideal viral loads exists for each type of organ transplantation, signifying optimal antirejection dosing.…”

Section: Introductionmentioning

confidence: 99%

Torquetenovirus Serum Load and Long-Term Outcomes in Renal Transplant Recipients

Gore

Gomes-Neto

Wang

et al. 2020

JCM

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Following transplantation, patients must take immunosuppressive medication for life. Torquetenovirus (TTV) is thought to be marker for immunosuppression, and TTV–DNA levels after organ transplantation have been investigated, showing high TTV levels, associated with increased risk of infections, and low TTV levels associated with increased risk of rejection. However, this has been investigated in studies with relatively short follow-up periods. We hypothesized that TTV levels can be used to assess long term outcomes after renal transplantation. Serum samples of 666 renal transplant recipients were tested for TTV DNA. Samples were taken at least one year after renal transplantation, when TTV levels are thought to be relatively stable. Patient data was reviewed for graft failure, all-cause mortality and death due to infectious causes. Our data indicates that high TTV levels, sampled more than one year post-transplantation, are associated with all-cause mortality with a hazard ratio (HR) of 1.12 (95% CI, 1.02–1.23) per log10 increase in TTV viral load, (p = 0.02). Additionally, high TTV levels were also associated with death due to infectious causes (HR 1.20 (95% CI 1.01–1.43), p = 0.04). TTV levels decrease in the years following renal transplantation, but remain elevated longer than previously thought. This study shows that TTV level may aid in predicting long-term outcomes, all-cause mortality and death due to an infectious cause in renal transplant patients sampled over one year post-transplantation.

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Torque teno virus (TTV) in immunosuppressed host: Performances studies of TTV R-Gene® kit and donors and recipients kidney samples genotyping

Cited by 2 publications

References 0 publications

Torque Teno Virus Viral Load as a Marker of Immune Function in Allogeneic Haematopoietic Stem Cell Transplantation Recipients

Torque Teno Virus Viral Load as a Marker of Immune Function in Allogeneic Haematopoietic Stem Cell Transplantation Recipients

Torquetenovirus Serum Load and Long-Term Outcomes in Renal Transplant Recipients

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