Torque teno virus in patients undergoing allogeneic hematopoietic stem cell transplantation for hematological malignancies

Masouridi‐Levrat, Stavroula; Pradier, Amandine; Simonetta, Federico; Kaiser, Laurent; Chalandon, Yves; Roosnek, Eddy

doi:10.1038/bmt.2015.262

Cited by 38 publications

(62 citation statements)

References 18 publications

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“…Fourth, a direct correlation between ALCs and plasma TTV DNA loads after engraftment has been reported . The data presented herein further support this assumption and extend it to TTV DNA load in saliva.…”

Section: Discussionsupporting

confidence: 84%

“…Moreover, the area under a curve of plasma TTV DNA loads between days +20 and +30 after allo‐HSCT allows the estimation of the risk of high‐level cytomegalovirus DNAemia . Nevertheless, the available data also suggest that once the TTV DNA load reaches a plateau, its magnitude may reflect the net state of immunosuppression, as rising plasma TTV DNA levels are seen in episodes of severe acute graft‐versus‐host disease requiring corticosteroid therapy at high doses …”

Section: Introductionmentioning

confidence: 99%

“…In fact, it has been shown that plasma TTV DNA load decreases dramatically as a result of conditioning and that following engraftment, it increases in parallel to the absolute lymphocyte cell counts (ALCs). [3][4][5][6] Moreover, the area under a curve of plasma TTV DNA loads between days +20 and +30 after allo-HSCT allows the estimation of the risk of high-level cytomegalovirus DNAemia. 7 Nevertheless, the available data also suggest that once the TTV DNA load reaches a plateau, its magnitude may reflect the net state of immunosuppression, as rising plasma TTV DNA levels are seen in episodes of severe acute graft-versus-host disease requiring corticosteroid therapy at high doses.…”

Section: Introductionmentioning

confidence: 99%

“…7 Nevertheless, the available data also suggest that once the TTV DNA load reaches a plateau, its magnitude may reflect the net state of immunosuppression, as rising plasma TTV DNA levels are seen in episodes of severe acute graft-versus-host disease requiring corticosteroid therapy at high doses. 5,6,8,9 Although T lymphocytes appear to be the main target for TTV replication, 10,11 other cell types of nonhematopoietic origin may also be fully permissive, thus potentially contributing to the TTV burden. 1,2 Pertinent to this study, TTV DNA is frequently detected in saliva from TTV-infected healthy individuals.…”

Section: Introductionmentioning

confidence: 99%

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Kinetics of torque teno virus DNA load in saliva and plasma following allogeneic hematopoietic stem cell transplantation

Albert

Torres

Talaya

et al. 2018

Journal of Medical Virology

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Plasma torque teno virus (TTV) DNA load directly correlates with the degree of T-cell immune reconstitution early after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Here, the kinetics of oral TTV DNA shedding was examined to assess whether quantitation of TTV DNA load in saliva may either replace or complement that in plasma for predicting lymphocyte (ALC) reconstitution after engraftment. This prospective observational study enrolled 38 nonconsecutive allo-HSCT recipients. Saliva and plasma specimens were collected at baseline (pretransplant) and at around days +30, +50, and +90 after allo-HSCT. TTV DNA was quantitated in both specimen types by real-time PCR. ALCs were measured by cytometry. A total of 104 paired saliva and plasma specimens were available for TTV PCR analyses. TTV DNA was detected more frequently in saliva than in plasma specimens at all time points (overall, 94.2% vs 86.5%). Increasing levels of TTV DNA were seen in both specimen types from day +30 to day +90 after transplantation. Overall, TTV DNA loads were significantly higher in saliva than in plasma specimens (P = .0002) and correlated significantly (P ≤ .0001). A direct correlation between TTV DNA loads in saliva and plasma and ALCs was observed after engraftment (P = .034 and P = .002, respectively). Future studies should be aimed at determining whether monitoring of oral TTV DNA shedding may be of any utility for inference of immune reconstitution after allo-HSCT.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Kinetics of torque teno virus DNA load in saliva and plasma following allogeneic hematopoietic stem cell transplantation

Albert

Torres

Talaya

et al. 2018

Journal of Medical Virology

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“…9 Anelloviridae are a family of highly diverse, nonenveloped, small circular single-stranded DNA (ssDNA) viruses that infect humans and other mammals. 14,16,17 Whether lung allograft rejection might be associated with low Anelloviridae levels within the lung itself, perhaps as an indicator of local immune function, has not been investigated. [10][11][12] Anelloviridae are likely under chronic immune control, given that levels in blood increase in immunosuppressed states.…”

Section: Introductionmentioning

confidence: 99%

Bidirectional transfer of Anelloviridae lineages between graft and host during lung transplantation

Abbas

Young

Clarke

et al. 2019

American Journal of Transplantation

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Solid organ transplantation disrupts virus-host relationships, potentially resulting in viral transfer from donor to recipient, reactivation of latent viruses, and new viral infections. Viral transfer, colonization, and reactivation are typically monitored using assays for specific viruses, leaving the behavior of full viral populations (the "virome") understudied. Here we sought to investigate the temporal behavior of viruses from donor lungs and transplant recipients comprehensively. We interrogated the bronchoalveolar lavage and blood viromes during the peritransplant period and 6-16 months posttransplant in 13 donor-recipient pairs using shotgun metagenomic sequencing. Anelloviridae, ubiquitous human commensal viruses, were the most abundant human viruses identified. Herpesviruses, parvoviruses, polyomaviruses, and bacteriophages were also detected. Anelloviridae populations were complex, with some donor organs and hosts harboring multiple contemporaneous lineages. We identified transfer of Anelloviridae lineages from donor organ to recipient serum in 4 of 7 cases that could be queried, and immigration of lineages from recipient serum into the allograft in 6 of 10 such cases. Thus, metagenomic analyses revealed that viral populations move between graft and host in both directions, showing that organ transplantation involves implantation of both the allograft and commensal viral communities.

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Better detection of Torque teno virus in children with leukemia by metagenomic sequencing than by quantitative PCR

Leijonhufvud

Bajalan

Soratto

et al. 2021

Journal of Medical Virology

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Torque teno virus (TTV) is a group of chronically persisting viruses with a short circular DNA genome. TTV demonstrates a wide sequence diversity and a large majority of humans are chronically infected by one or more types of TTV. As TTV is ubiquitous, and viral replication correlates with immune status, TTV has been studied as a marker to assess global functional immune competence in transplant recipients. Most studies of the prevalence, amounts, and variation in TTV have been performed using PCR assays. We here present a comparison of the most frequently used quantitative PCR (qPCR) assay for TTV with shotgun metagenomic sequencing for detection and characterization of TTV in a cohort of pediatric cancer patients.The results show that TTV is more common than the qPCR assays indicate, and analysis of the TTV genome sequences indicate that a qPCR with primers and probe designed on a conserved region of the TTV genome may fail to detect some of the TTV strains found in this study.

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Torque teno virus in patients undergoing allogeneic hematopoietic stem cell transplantation for hematological malignancies

Cited by 38 publications

References 18 publications

Kinetics of torque teno virus DNA load in saliva and plasma following allogeneic hematopoietic stem cell transplantation

Kinetics of torque teno virus DNA load in saliva and plasma following allogeneic hematopoietic stem cell transplantation

Bidirectional transfer of Anelloviridae lineages between graft and host during lung transplantation

Better detection of Torque teno virus in children with leukemia by metagenomic sequencing than by quantitative PCR

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