Torque Teno Viral Plasma Load for Immunologic Monitoring in Solid Organ Transplantation: One Step Further

Haupenthal, Frederik; Bond, Gregor

doi:10.1097/tp.0000000000004817

Cited by 4 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, in solid organ transplant recipients, advanced age, mycophenolate use, triple immunosuppression, and more advanced stages of organ failure are the only reliable predictors of response to COVID-19 vaccines [37,38,41,[44][45][46]. While there are discordant results for time since transplantation [37,38].…”

Section: Discussionmentioning

confidence: 99%

Torquetenovirus Loads in Peripheral Blood Predict Both the Humoral and Cell-Mediated Responses to SARS-CoV-2 Elicited by the mRNA Vaccine in Liver Transplant Recipients

Minosse,

Matusali,

Meschi

et al. 2023

Vaccines

View full text Add to dashboard Cite

Three years into the COVID-19 pandemic, mass vaccination campaigns have largely controlled the disease burden but have not prevented virus circulation. Unfortunately, many immunocompromised patients have failed to mount protective immune responses after repeated vaccinations, and liver transplant recipients are no exception. Across different solid organ transplant populations, the plasma levels of Torquetenovirus (TTV), an orphan and ubiquitous human virus under control of the immune system, have been shown to predict the antibody response after COVID-19 vaccinations. We show here a single-institution experience with TTV viremia in 134 liver transplant recipients at their first or third dose. We found that TTV viremia before the first and third vaccine doses predicts serum anti-SARS-CoV-2 Spike receptor-binding domain (RBD) IgG levels measured 2–4 weeks after the second or third dose. Pre-vaccine TTV loads were also associated with peripheral blood anti-SARS-CoV-2 cell-mediated immunity but not with serum SARS-CoV-2 neutralizing antibody titers.

show abstract

Section: Discussionmentioning

confidence: 99%

Torquetenovirus Loads in Peripheral Blood Predict Both the Humoral and Cell-Mediated Responses to SARS-CoV-2 Elicited by the mRNA Vaccine in Liver Transplant Recipients

Minosse,

Matusali,

Meschi

et al. 2023

Vaccines

View full text Add to dashboard Cite

show abstract

“…Each of these viruses consists of numerous species and strains. The recent evidence that TTV represents the most abundant component of the human virome and establishes a highly successful interaction with the infected host [ 1 ] has highlighted the importance of using the monitoring of TTV viremia as a biomarker of functional immune competence [ 9 , 10 , 11 , 12 , 13 , 14 ]. Thus, the use of diagnostic tests that can be performed for rapid and sensitive quantification of TTV is of utmost importance for follow-up monitoring and therapy decisions in several patient settings, notably solid organ transplant recipients [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Torquetenovirus Viremia Quantification Using Real-Time PCR Developed on a Fully Automated, Random-Access Platform

Spezia,

Carletti,

Novazzi

et al. 2024

Viruses

View full text Add to dashboard Cite

Quantification of Torquetenovirus (TTV) viremia is becoming important for evaluating the status of the immune system in solid organ transplant recipients, monitoring the appearance of post-transplant complications, and controlling the efficacy of maintenance immunosuppressive therapy. Thus, diagnostic approaches able to scale up TTV quantification are needed. Here, we report on the development and validation of a real-time PCR assay for TTV quantification on the Hologic Panther Fusion® System by utilizing its open-access channel. The manual real-time PCR previously developed in our laboratories was optimized to detect TTV DNA on the Hologic Panther Fusion® System. The assay was validated using clinical samples. The automated TTV assay has a limit of detection of 1.6 log copies per ml of serum. Using 112 samples previously tested via manual real-time PCR, the concordance in TTV detection was 93% between the assays. When the TTV levels were compared, the overall agreement between the methods, as assessed using Passing–Bablok linear regression and Bland–Altman analyses, was excellent. In summary, we validated a highly sensitive and accurate method for the diagnostic use of TTV quantification on a fully automated Hologic Panther Fusion® System. This will greatly improve the turnaround time for TTV testing and better support the laboratory diagnosis of this new viral biomarker.

show abstract

Torque Teno Virus–guided monitoring of immunosuppressive therapy

Kapps,

Haupenthal,

Bond

2024

Nephrology Dialysis Transplantation

View full text Add to dashboard Cite

Torque Teno Viral Plasma Load for Immunologic Monitoring in Solid Organ Transplantation: One Step Further

Cited by 4 publications

References 10 publications

Torquetenovirus Loads in Peripheral Blood Predict Both the Humoral and Cell-Mediated Responses to SARS-CoV-2 Elicited by the mRNA Vaccine in Liver Transplant Recipients

Torquetenovirus Loads in Peripheral Blood Predict Both the Humoral and Cell-Mediated Responses to SARS-CoV-2 Elicited by the mRNA Vaccine in Liver Transplant Recipients

Torquetenovirus Viremia Quantification Using Real-Time PCR Developed on a Fully Automated, Random-Access Platform

Torque Teno Virus–guided monitoring of immunosuppressive therapy

Contact Info

Product

Resources

About