TOR signalling regulates epithelial cell shape transition in<i>Drosophila</i>oogenesis

Halder, Sudipta; Ghosh, Gaurab; Gayen, Budhaditya; Prasad, Mohit

doi:10.1101/2022.12.29.522192

Cited by 2 publications

(1 citation statement)

References 59 publications

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“…Next, we wondered if EcR DN over expressing AFCs, are deficient in removing the lateral protein Fascilin 2? Fasciclin 2 (Fas2) is a cell adhesion molecule from the lateral domains and its timely removal is requisite for transition of AFCs from cuboidal to squamous fate (Gomez et al, 2012;Halder et al). Generally, Fas2 is expressed till stage 7, after which Fas2 is removed from the lateral membrane of anterior follicle cells (Gomez et al, 2012;Halder et al 2022).…”

Section: Ecdysone Requires For Cell Junction Remodellingmentioning

confidence: 99%

Ecdysone Orchestrates Notch and Broad Symphony to Craft Epithelial Cell Shape Change

Ghosh,

Halder,

Sahu

et al. 2023

Preprint

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Morphogenesis in the metazoans relies on cell shape transformations that forms an integral component of organ development, form generation and maintenance of tissue homeostasis. Nevertheless, a comprehensive grasp of how the epithelial morphogenesis is modulated in the metazoans remain still elusive. The Steroid hormones play a pivotal role in morphogenesis spanning several organs including the gonads, urogenital tracts, and mammary glands. Employing the Drosophila oogenesis model, we investigated the the role of steroid hormone receptor, Ecdysone receptor (EcR) involvement in transforming anterior epithelial follicle cells (AFCs) from cuboidal to squamous shape. Consistent with the fact that the activity of EcR in the AFCs coincides with the timing of cuboidal-to-squamous shape transition, we found that depletion of EcR function impedes the shape transformation of AFCs. We report that EcR doesnot impair the follicle cell fate, but impedes the morphological change by restricting the remodelling of lateral and adherens junctions. Employing the classical genetic tools and immnohistochemistry, we show that EcR limits the Notch-Broad axis to facilitate alteration of the shape of AFCs. Our study suggests a mechanistic model where Ecdysone signalling, via the Notch pathway, finetunes the activity of non-muscle myosin heavy chain zipper, prompting AFC shape transition. In sum, our work illuminates how Ecdysone signalling orchestrates epithelial follicle cell morphogenesis during metazoan development.

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Section: Ecdysone Requires For Cell Junction Remodellingmentioning

confidence: 99%

Ecdysone Orchestrates Notch and Broad Symphony to Craft Epithelial Cell Shape Change

Ghosh,

Halder,

Sahu

et al. 2023

Preprint

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Hippo-TOR Signaling Crosstalks Underpin Microtubule Acetylation-linked Carcinogenesis inDrosophilaSquamous Epithelia

Bhattacharya,

Sinha,

Mohan

2024

Preprint

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Post-translational modifications (PTM), like acetylation, underpin the functional specialization of the cytoskeleton, such as microtubules (MT). For instance, acetylation of microbules is essential for cell flattening inthe Drosophilaadult squamous epithelia of female ovarian follicles and male accessory glands (MAG). Here we show that the highly conserved Hippo transcription co-factor Yorkie (Yki), a mechanosensor for cell flattening, regulates the acetylation of MT in squamous epithelia in both these organs. Yet, the fallouts of loss of nuclear Yki signaling are distinct in these two squamous epithelia. Thus, the knockdown of Yki in the squamous epithelia of ovarian follicles compromises their cell flattening. By contrast, knockdown of Yki in the MAG epithelium leads to its cancerous transformation which is suppressed by a simultaneous genetic ablation of MT-acetylation. We further note that Yki-knockdown-induced hyperacetylation of MT in MAG is TOR signaling-dependent. These results reveal that, while Yki-dependent acetylation of MT drives cell flattening, its cell type-specific homeostatic cross-talk with TOR signaling, as in the MAG, when dysregulated, culminates in carcinogenesis.

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TOR signalling regulates epithelial cell shape transition inDrosophilaoogenesis

Cited by 2 publications

References 59 publications

Ecdysone Orchestrates Notch and Broad Symphony to Craft Epithelial Cell Shape Change

Ecdysone Orchestrates Notch and Broad Symphony to Craft Epithelial Cell Shape Change

Hippo-TOR Signaling Crosstalks Underpin Microtubule Acetylation-linked Carcinogenesis inDrosophilaSquamous Epithelia

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