Topography and function of the mesenteric capillary circulation

Chambers, Robert; Zweifach, Benjamin W.

doi:10.1002/aja.1000750204

Cited by 361 publications

(114 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mesentery model has also been used to study the effects of various hormones, mediators, drugs, and other environmental impacts on microcirculation, including the influence of histamine, norepinephrine, dopamine, dobutamine, PO2, substance P, L-NAME, methylene blue, leukotrienes, histamine, platelet activating factor, temperature, low-power laser radiation, X-radiation, and others [3,20,[27][28][29][30][31][32][33][34] . In particular, this model was used in the first study of the effects of nitric oxide (NO) on lymph microvessels in vivo: Shirasawa et al [35] showed the influence of a NO synthase inhibitor on lymphatic diameter and contractile activity, which was reversed after applying an endogenous NO donor (L-arginine).…”

Section: Introductionmentioning

confidence: 44%

Section: Introductionmentioning

confidence: 47%

“…During the first 30-50 years of the 20th century, extensive in vivo studies of mesenteric microcirculation were undertaken [2][3][4] . In particular, the basics of capillary circulation were first studied in rat mesentery [3] . Later, this model was successfully used to study the fundamentals of the microvascular physiology of blood (i.e., microvascular rheolog y, hemodynamics, vasomotion, hematocrit, permeability of the vascular wall, flow velocity) and lymph (i.e., phasic contractile activity, flow velocity, and the diameter of small lymphatics) systems .…”

Section: Introductionmentioning

confidence: 47%

See 2 more Smart Citations

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

View full text Add to dashboard Cite

Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

show abstract

Section: Introductionmentioning

confidence: 44%

Section: Introductionmentioning

confidence: 47%

Section: Introductionmentioning

confidence: 47%

See 1 more Smart Citation

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

View full text Add to dashboard Cite

show abstract

“…3 ± 5 The vasomotion has been observed directly only in the vessels of the mesentery 15,16 and the wing of the bat. 17 The vasomotion in the spinal cord, however, can be more accurately determined when observed stereoscopically using vascular corrosion casts and a scanning electron microscope, 6 ± 9,14 and it is becoming better understood through immunohistochemical studies.…”

Section: Discussionmentioning

confidence: 99%

Regulation of vasomotion of arterioles and capillaries in the cat spinal cord: role of α actin and endothelin-1

et al. 1997

View full text Add to dashboard Cite

Ring-shaped vasoconstrictions of arterioles at their branching sites have often been reported in vascular corrosion casts of the brain and spinal cord in rats and cats. It is surmised that smooth muscle cells in arteriolar walls could regulate the blood¯ow by changing the diameter of the lumen (ie vasomotion). However, few reports have described vasomotion at the capillary (capillaries have no smooth muscle cells). Also, there have been no reports on endothelin-1 in the arterioles and capillaries of the spinal cord. This study was designed to determine (1) the electron microscopic architecture of vasomotion; (2) the immunohistochemical identi®cation of a actin and endothelin-1 in the arterioles and capillaries of the spinal cord. Twenty-seven adult mongrel cats were used to study vascular corrosion casts at the lumbosacral spinal cord segments immunohistologically and through scanning electron microscopic observations. Sections of the spinal cord were stained with monoclonal anti-a actin and endothelin-1 antibodies.Vascular corrosion casts demonstrated two types of vasomotion: a sausage-like peristalsis and a ring-shaped vasoconstriction at the arteriole and capillary levels. In the immunohistological study, a actin and endothelin-1 were identi®able in the vascular wall at the bifurcation, and pericytes were found to contain micro®laments of a actin. The ringshaped vasoconstriction might be regulated by smooth muscle cells in arterioles and by pericytes in capillaries by releasing endothelin-1.

show abstract

“…Capillaries were devoid of vascular smooth muscle cells, contained flowing frog red blood cells (fRBC) and had no rolling or sticking WBC. At the branching ends of each capillary the direction of fRBC flow was noted and each capillary was categorized (Chambers and Zweifach, 1944). TC (true capillaries with divergent flow at one end and convergent flow at the other end) and VC (venular capillaries with convergent flow at both ends) were used in this study.…”

Section: Intravital Video Microscopymentioning

confidence: 99%

Change in shear stress (Δτ)/hydraulic conductivity (Lp) relationship after pronase treatment of individual capillaries in situ

Williams

2007

Microvascular Research

View full text Add to dashboard Cite

A complex glycoprotein meshwork covers the inner wall of blood vessels and is implicated in mechanotransduction of fluid shear stress (τ). A relationship between Δτ and capillary L p has been established. The purpose of this study was to evaluate L p in response to Δτ after exposing the capillary lumen to a mild, non-specific protease selected to disrupt its inner matrix. We hypothesized that L p would not correlate with Δτ after enzyme treatment. Frogs (Rana pipiens, n=69) were pithed and the mesentery was exteriorized. L p was assessed at 30 cm H 2 O using the modified Landis technique after an abrupt, square wave Δτ produced by a physiologically relevant increase in pressure. Perfusate solutions were 10 mg·ml −1 BSA/frog Ringer's (Control) or 0.1 mg·ml −1 pronase in BSA/Ringer's (1 min) then BSA/Ringer's alone (Test). Mean (±SE) control L p following Δτ was 2.2±0.2 x 10 −7 cm·s −1 ·cm H 2 O-1 and individual values correlated positively with Δτ (r=0.85, P<0.0001, n=41). After pronase, mean Test L p (17.6±2.5 x 10 −7 cm·s −1 ·cm H 2 O-1) was higher compared to control and Δτ/L p plots revealed two subsets of capillaries. L p correlated strongly with Δτ in capillaries with diameters ≤ 15 m (r=0.91, P=0.0006, n=14) and also in a second subset of capillaries with diameters >15 m (r=0.96, P=0.0001, n=8). Slopes were 3.9-and 8.7-fold higher, respectively, compared to control. These data suggest a protective role for luminal constituents of intact capillaries. Mechanisms involved in capillary responses to flow-induced, mechanical stimuli may be located in the cellular structures that form capillaries.

show abstract

Topography and function of the mesenteric capillary circulation

Cited by 361 publications

References 25 publications

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Regulation of vasomotion of arterioles and capillaries in the cat spinal cord: role of α actin and endothelin-1

Change in shear stress (Δτ)/hydraulic conductivity (Lp) relationship after pronase treatment of individual capillaries in situ

Contact Info

Product

Resources

About