Topographical mapping of sympathetic postganglionic innervation of mouse heart v1

Bizanti, Ariege; Zhang, Yuanyuan; Bendowski, Kohlton; Chen, Jin; Osanlouy, Mahyar

doi:10.17504/protocols.io.b2ebqban

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“…The original high quality figures and data associated with this study 83 , are available through the SPARC Portal (RRID: SCR_017041) under a CC-BY 4.0 license. https:// doi.…”

Section: Data Availabilitymentioning

confidence: 99%

Topographical mapping of catecholaminergic axon innervation in the flat-mounts of the mouse atria: a quantitative analysis

Zhang

Bizanti

Harden

et al. 2023

Sci Rep

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The sympathetic nervous system is crucial for controlling multiple cardiac functions. However, a comprehensive, detailed neuroanatomical map of the sympathetic innervation of the heart is unavailable. Here, we used a combination of state-of-the-art techniques, including flat-mount tissue processing, immunohistochemistry for tyrosine hydroxylase (TH, a sympathetic marker), confocal microscopy and Neurolucida 360 software to trace, digitize, and quantitatively map the topographical distribution of the sympathetic postganglionic innervation in whole atria of C57Bl/6 J mice. We found that (1) 4–5 major extrinsic TH-IR nerve bundles entered the atria at the superior vena cava, right atrium (RA), left precaval vein and the root of the pulmonary veins (PVs) in the left atrium (LA). Although these bundles projected to different areas of the atria, their projection fields partially overlapped. (2) TH-IR axon and terminal density varied considerably between different sites of the atria with the greatest density of innervation near the sinoatrial node region (P < 0.05, n = 6). (3) TH-IR axons also innervated blood vessels and adipocytes. (4) Many principal neurons in intrinsic cardiac ganglia and small intensely fluorescent cells were also strongly TH-IR. Our work provides a comprehensive topographical map of the catecholaminergic efferent axon morphology, innervation, and distribution in the whole atria at single cell/axon/varicosity scale that may be used in future studies to create a cardiac sympathetic-brain atlas.

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“…The original high quality figures and data associated with this study 83 , are available through the SPARC Portal (RRID: SCR_017041) under a CC-BY 4.0 license. https:// doi.…”

Section: Data Availabilitymentioning

confidence: 99%

Topographical mapping of catecholaminergic axon innervation in the flat-mounts of the mouse atria: a quantitative analysis

Zhang

Bizanti

Harden

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

Topographical mapping of sympathetic postganglionic innervation of mouse heart v1

Abstract: This protocol describes the process of mapping the topographical organization of tyrosine hydroxylase immune reactive sympathetic postganglionic axons and terminals in the mouse heart. Hearts were removed and separated as whole mounts, then scanned using confocal or zeiss microscopy

Cited by 1 publication

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Topographical mapping of catecholaminergic axon innervation in the flat-mounts of the mouse atria: a quantitative analysis

Topographical mapping of catecholaminergic axon innervation in the flat-mounts of the mouse atria: a quantitative analysis

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