Topographical distribution and morphology of SP-IR axons in the antrum, pylorus, and duodenum of mice

Mistareehi, Anas; Bendowski, Kohlton; Bizanti, Ariege; Madas, Jazune; Zhang, Yuanyuan; Kwiat, Andrew; Nguyen, Duyen; Kogut, Nicole; Ma, Jichao; Chen, Jin; Cheng, Zixi

doi:10.1016/j.autneu.2023.103074

Cited by 2 publications

(1 citation statement)

References 98 publications

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“…In addition to the types that have been identified, we believe there exists other groups of spinal afferents that innervate other layers of the stomach such as the submucosa and mucosa 21 . While the scope of this study did not go beyond the muscular layers of the stomach, it is feasible to examine the spinal afferent innervation across layers by either separating the submucosa as a whole flat-mount or utilizing sectioned tissues, both techniques were well established in our laboratory 28 , 56 .…”

Section: Discussionmentioning

confidence: 99%

Spinal afferent innervation in flat-mounts of the rat stomach: anterograde tracing

Ma,

Nguyen,

Madas

et al. 2023

Sci Rep

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The dorsal root ganglia (DRG) project spinal afferent axons to the stomach. However, the distribution and morphology of spinal afferent axons in the stomach have not been well characterized. In this study, we used a combination of state-of-the-art techniques, including anterograde tracer injection into the left DRG T7-T11, avidin–biotin and Cuprolinic Blue labeling, Zeiss M2 Imager, and Neurolucida to characterize spinal afferent axons in flat-mounts of the whole rat stomach muscular wall. We found that spinal afferent axons innervated all regions with a variety of distinct terminal structures innervating different gastric targets: (1) The ganglionic type: some axons formed varicose contacts with individual neurons within myenteric ganglia. (2) The muscle type: most axons ran in parallel with the longitudinal and circular muscles and expressed spherical varicosities. Complex terminal structures were observed within the circular muscle layer. (3) The ganglia-muscle mixed type: some individual varicose axons innervated both myenteric neurons and the circular muscle, exhibiting polymorphic terminal structures. (4) The vascular type: individual varicose axons ran along the blood vessels and occasionally traversed the vessel wall. This work provides a foundation for future topographical anatomical and functional mapping of spinal afferent axon innervation of the stomach under normal and pathophysiological conditions.

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Section: Discussionmentioning

confidence: 99%

Spinal afferent innervation in flat-mounts of the rat stomach: anterograde tracing

Ma,

Nguyen,

Madas

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

Spinal Afferent Innervation in Flat-Mounts of the Rat Stomach: Anterograde Tracing

Nguyen

Madas

et al. 2023

Preprint

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The dorsal root ganglia (DRG) project spinal afferent axons to the stomach. However, the distribution and morphology of spinal afferent axons in the stomach have not been well characterized. In this study, we used a combination of state-of-the-art techniques, including anterograde tracer injection into the left DRG T7-T11, avidin-biotin and Cuprolinic Blue labeling, Zeiss M2 Imager, and Neurolucida to characterize spinal afferent axons in the flat-mounts of the whole rat stomach muscular wall. We found that spinal afferent axons innervated all regions with a variety of distinct terminal structures innervating different gastric targets: 1) The ganglionic type: some axons formed varicose contacts with individual neurons within myenteric ganglia. 2) The muscle type: most axons ran in parallel with the longitudinal and circular muscles and expressed spherical varicosities. Complex terminal structures were observed within the circular muscle layer. 3) The ganglia-muscle mixed type: some individual varicose axons innervated both myenteric ganglia and circular muscles, exhibiting polymorphic terminal structures. 4) The vascular type: individual varicose axons ran along the blood vessels and occasionally traversed the vessel wall. This work provides a foundation for future topographical anatomical and functional mapping of spinal afferent axon innervation of the stomach under normal and pathophysiological conditions.

show abstract

Topographical distribution and morphology of SP-IR axons in the antrum, pylorus, and duodenum of mice

Cited by 2 publications

References 98 publications

Spinal afferent innervation in flat-mounts of the rat stomach: anterograde tracing

Spinal afferent innervation in flat-mounts of the rat stomach: anterograde tracing

Spinal Afferent Innervation in Flat-Mounts of the Rat Stomach: Anterograde Tracing

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