Topographical and Physicochemical Modification of Material Surface to Enable Patterning of Living Cells

Jung, Dong Soo; Kapur, Ravi; Adams, Terri; Giuliano, Kenneth A.; Mrksich, Milan; Craighead, Harold G.; Taylor, D. Lansing

doi:10.1080/20013891081700

Cited by 170 publications

(100 citation statements)

References 237 publications

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“…Cell alignment and elongation of different cell types have been studied on micro- [15,17,18,21,25,[34][35][36][37][38][39][40] or nanoscale gratings [22]. Cell alignment, F-actin alignment and elongation are usually observed on the edges of micro-ridges.…”

Section: Discussionmentioning

confidence: 99%

Nanopattern-induced changes in morphology and motility of smooth muscle cells

Yim¹,

et al. 2005

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Cells are known to be surrounded by nanoscale topography in their natural extracellular environment. The cell behavior, including morphology, proliferation, and motility of bovine pulmonary artery smooth muscle cells (SMC) were studied on poly(methyl methacrylate) (PMMA) and poly (dimethylsiloxane) (PDMS) surfaces comprising nanopatterned gratings with 350 nm linewidth, 700 nm pitch, and 350 nm depth. More than 90% of the cells aligned to the gratings, and were significantly elongated compared to the SMC cultured on non-patterned surfaces. The nuclei were also elongated and aligned. Proliferation of the cells was significantly reduced on the nanopatterned surfaces. The polarization of microtubule organizing centers (MTOC), which are associated with cell migration, of SMC cultured on nanopatterned surfaces showed a preference towards the axis of cell alignment in an in vitro wound healing assay. In contrast, the MTOC of SMC on non-patterned surfaces preferentially polarized towards the wound edge. It is proposed that this nanoimprinting technology will provide a valuable platform for studies in cell-substrate interactions and for development of medical devices with nanoscale features.

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Section: Discussionmentioning

confidence: 99%

Nanopattern-induced changes in morphology and motility of smooth muscle cells

Yim¹,

et al. 2005

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“…[1][2][3][4][5][6][7][8][9][10][11][12] This has led to the discovery that micro-and nanotopology causes cells to elongate, [9] align to the direction of the surface pattern, [9,[13][14] to rearrange the extracellular matrix in contact with the surface, [11,15] and to internally re-organize cellular components. [9,12] It is also possible that the topography can illicit varying cellular responses.…”

Section: Introductionmentioning

confidence: 99%

Directional Alignment of MG63 Cells on Polymer Surfaces Containing Point Microstructures

Mills

Fernandez

Martínez

et al. 2007

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MG63 cells cultured on regular arrays of point microstructures (posts and holes) are shown to preferentially align at certain angles to the pattern of the structures, at 08, 308, and 458 in particular. The effect is found to be more pronounced for post rather than hole structures (although no significant difference is found for the angles the cells make to the holes or posts) and is thought to be due to the fact that the cells use the posts as anchorage points to hold themselves to the surface. It is also shown that cells preferentially align with the structures depending on the dimensions of the structures and the distance between neighboring structures. This is important when designing structured surfaces for cellsurface interaction studies for materials to be used in, for example, drug delivery or tissue engineering.

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“…In the field of biomaterial development and in vivo implant technology, the nanoscale structure and morphologenic factor of the surface have played a critical role in accelerating the rate of cell proliferation and enhancing tissue acceptance with a reduced immune response (1,2). In terms of in vitro cell biology, there has also been much attention placed on cellular responses to their structural surroundings (3).…”

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confidence: 99%

Stem cell fate dictated solely by altered nanotube dimension

Oh¹,

Brammer²,

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

1,096

914

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Two important goals in stem cell research are to control the cell proliferation without differentiation and to direct the differentiation into a specific cell lineage when desired. Here, we demonstrate such paths by controlling only the nanotopography of culture substrates. Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion or a specific differentiation of hMSCs into osteoblasts by using only the geometric cues, absent of osteogenic inducing media. hMSC behavior in response to defined nanotube sizes revealed a very dramatic change in hMSC behavior in a relatively narrow range of nanotube dimensions. Small (Ϸ30-nm diameter) nanotubes promoted adhesion without noticeable differentiation, whereas larger (Ϸ70-to 100-nm diameter) nanotubes elicited a dramatic stem cell elongation (Ϸ10-fold increased), which induced cytoskeletal stress and selective differentiation into osteoblast-like cells, offering a promising nanotechnology-based route for unique orthopedics-related hMSC treatments.differentiation ͉ mesenchymal ͉ nanotopography ͉ osteogenesis ͉ proliferation N anostructures are of particular interest because they have the advantageous feature of a high surface-to-volume ratio, and they elicit a higher degree of biological plasticity compared with conventional micro-or macrostructures. In the field of biomaterial development and in vivo implant technology, the nanoscale structure and morphologenic factor of the surface have played a critical role in accelerating the rate of cell proliferation and enhancing tissue acceptance with a reduced immune response (1, 2). In terms of in vitro cell biology, there has also been much attention placed on cellular responses to their structural surroundings (3). In fact, it has been observed that macro-, micro-and nano-sized topographical factors stimulate behavioral changes in both cells and tissues. Recent studies related to the effect of nanotopography on cellular behavior indicated that osteoblast adhesion and functionality was enhanced by 30% when cultured on a nanograined Al 2 O 3 and TiO 2 substrate (4-6) compared with those cultured on a micrograined surface, and nanostructures such as TiO 2 nanotubes with Ͻ100-nm spacing showed superior characteristics in bone mineral synthesis (5). However, most of the previous studies on nanostructures and cell responses have mainly used oriented, patterned, or semiordered polymer arrays (7-9) and alumina/ polymer hybrid patterned arrays (10).The material and mechanical characteristics of titanium (Ti) metal, which has a thin native oxide layer of TiO 2 , make it an ideal orthopedic material that bonds directly to the adjacent bone surface (11,12). Fabrication of the nanostructured titanium dioxide (TiO 2 ) nanotube arrays has been a primary subject of investigation lately because of the wide range of TiO 2 applications in the fields of solar cells (13-16), photocatalysis (17-19), photoelectrolysis (20), sensors (21,22), and b...

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Topographical and Physicochemical Modification of Material Surface to Enable Patterning of Living Cells

Cited by 170 publications

References 237 publications

Nanopattern-induced changes in morphology and motility of smooth muscle cells

Nanopattern-induced changes in morphology and motility of smooth muscle cells

Directional Alignment of MG63 Cells on Polymer Surfaces Containing Point Microstructures

Stem cell fate dictated solely by altered nanotube dimension

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