Topographic Restriction of TAG-1 Expression in the Developing Retinotectal Pathway and Target Dependent Reexpression during Axon Regeneration

Lang, Dirk; Warren, James T.; Klisa, Christiane; Stuermer, Claudia A. O.

doi:10.1006/mcne.2000.0936

Cited by 34 publications

(32 citation statements)

References 53 publications

(85 reference statements)

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“…Of these genes, gap43 (Reinhard et al, 1994) and tag1/axonin (cntn2 -Zebrafish Information Network) are expressed by migrating cerebellar granule cell precursors (Fig. 3E,G) (Console-Bram et al, 1996;Wolfer et al, 1994;Lang et al, 2001). In mutant embryos, no expression of these markers can be detected in the region that would give rise to the cerebellum, at any of the stages investigated (Fig.…”

Section: Lack Of Cerebellar Development In Acementioning

confidence: 94%

Isthmus-to-midbrain transformation in the absence of midbrain-hindbrain organizer activity

et al. 2003

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In zebrafish acerebellar (ace) embryos, because of a point mutation in fgf8, the isthmic constriction containing the midbrain-hindbrain boundary (MHB) organizer fails to form. The mutants lack cerebellar development by morphological criteria, and they appear to have an enlarged tectum, showing no obvious reduction in the tissue mass at the dorsal mesencephalic/metencephalic alar plate. To reveal the molecular identity of the tissues located at equivalent rostrocaudal positions along the neuraxis as the isthmic and cerebellar primordia in wild-types, we undertook a detailed analysis of ace embryos. In ace mutants, the appearance of forebrain and midbrain specific marker genes (otx2, dmbx1, wnt4) in the caudal tectal enlargement reveals a marked rostralized gene expression profile during early somitogenesis, followed by the lack of early and late cerebellar-specific gene expression (zath1/atoh1, gap43,tag1/cntn2, neurod, zebrin II). The Locus coeruleus(LC) derived from rostral rhombomere 1 is also absent in the mutants. A new interface between otx2 and epha4a suggests that the rostralization stops at the caudal part of rhombomere 1. The mesencephalic basal plate is also affected in the mutant embryos, as indicated by the caudal expansion of the diencephalic expression domains of epha4a,zash1b/ashb, gap43 and tag1/cntn2, and by the dramatic reduction of twhh expression. No marked differences are seen in cell proliferation and apoptotic patterns around the time the rostralization of gene expression becomes evident in the mutants. Therefore,locally distinct cell proliferation and cell death is unlikely to be the cause of the fate alteration of the isthmic and cerebellar primordia in the mutants. Dil cell-lineage labeling of isthmic primordial cells reveals that cells, at the location equivalent of the wild-type MHB, give rise to caudal tectum in ace embryos. This suggests that a caudalto-rostral transformation leads to the tectal expansion in the mutants. Fgf8-coated beads are able to rescue morphological MHB formation, and elicit the normal molecular identity of the isthmic and cerebellar primordium in ace embryos. Taken together, our analysis reveals that cells of the isthmic and cerebellar primordia acquire a more rostral, tectal identity in the absence of the functional MHB organizer signal Fgf8.

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Section: Lack Of Cerebellar Development In Acementioning

confidence: 94%

Isthmus-to-midbrain transformation in the absence of midbrain-hindbrain organizer activity

et al. 2003

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“…Zebrafish embryos were processed for whole-mount immunohistochemistry as previously described (Marx et al, 2001;Weiland et al, 1997). Embryos were labeled with zebrafish pAb Tag-1 (axons, Lang et al, 2001), anti-␣-actinin (Biotrend), antiphospho-Tyrosin (myotome boundaries, Santa Cruz Biotechnologiy), and phalloidin-Alexa 488 (actin, Invitrogen). Embryos were analyzed using a confocal microscope (LSM 510, Zeiss) equipped with a high aperture lens 40ϫ/1.2W (C-Apochromat, Zeiss) and the appropriate lasers.…”

Section: Whole Mounts In Situ Hybridization and Immunostainingmentioning

confidence: 99%

Molecular evolution and expression of zebrafish St8SiaIII, an alpha‐2,8‐sialyltransferase involved in myotome development

Bentrop

Marx

Schattschneider

et al. 2008

Developmental Dynamics

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Enzymes of the St8Sia family, a subgroup of the glycosyltransferases, mediate the transfer of sialic acid to glycoproteins or glycolipids. Here, we describe the cloning of the zebrafish St8SiaIII gene and study its developmental activity. A conserved synteny relationship among vertebrate chromosome regions containing St8SiaIII loci underscores an ancient duplication of this gene in the teleost fish lineage and a specific secondary loss of one paralog in the zebrafish. The single zebrafish St8SiaIII enzyme, which is expected to function as an oligosialyltransferase, lacks maternal activity, is weakly expressed during nervous system development, and shows a highly dynamic expression pattern in somites and somitederived structures. Morpholino knock-down of St8SiaIII leads to anomalous somite morphologies, including defects in segment boundary formation and myotendious-junction integrity. These phenotypes hint for a basic activity of zebrafish St8SiaIII during segmentation and somite formation, providing novel evidence for a non-neuronal function of sialyltransferases during vertebrate development. Developmental Dynamics 237:808 -818, 2008.

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“…TAG-1 can bind homophilically or heterophilically with other members of the Ig superfamily to mediate adhesion or signaling between cells or axons (Furley et al, 1990;Kuhn et al, 1991;Rader et al, 1993;Felsenfeld et al, 1994;Fitzli et al, 2000;Perrin et al, 2001;Pavlou et al, 2002;Karagogeos, 2003). We examined TAG-1 protein distribution on R-B axons by labeling with an anti-TAG-1 antibody (Lang et al, 2001). TAG-1 protein was expressed strongly on the central R-B axons at all stages that were examined, 20 -27 hpf ( Fig.…”

Section: Tag-1 Is Required For Central But Not Peripheral R-b Axon mentioning

confidence: 99%

Central and Peripheral Axon Branches from One Neuron Are Guided Differentially by Semaphorin3D and Transient Axonal Glycoprotein-1

Liu¹,

Halloran²

2005

J. Neurosci.

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For multiple axons from one neuron to extend in different directions to unique targets, the growth cones of each axon must have distinct responses to guidance cues. However, the mechanisms by which separate axon branches are guided along different pathways are mainly unknown. Zebrafish Rohon-Beard (R-B) sensory neurons extend central axon branches in the spinal cord and peripheral axons to the epidermis. To investigate the differential guidance mechanisms of the central versus peripheral R-B axon branches, we used live-growth cone imaging in vivo combined with manipulation of individual guidance molecules. We show that a semaphorin expressed at the dorsal spinal cord midline, Semaphorin3D (Sema3D), may act to repel the peripheral axons out of the spinal cord. Sema3D knock-down reduces the number of peripheral axons. Remarkably, Sema3D ectopic expression repels and induces branching of peripheral axons in vivo but has no effect on central axons from the same neurons. Conversely, central axons require a growth-promoting molecule, transient axonal glycoprotein-1 (TAG-1), to advance, whereas peripheral axons do not. After TAG-1 knock-down, central growth cones display extensive protrusive activity but make little forward advance. TAG-1 knock-down has no effect on the motility or advance of peripheral growth cones. These experiments show how Sema3D and TAG-1 regulate the motility and behavior of growth cones extending in their natural in vivo environment and demonstrate that two different axon branches from one neuron respond differently to guidance cues in vivo.

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Topographic Restriction of TAG-1 Expression in the Developing Retinotectal Pathway and Target Dependent Reexpression during Axon Regeneration

Cited by 34 publications

References 53 publications

Isthmus-to-midbrain transformation in the absence of midbrain-hindbrain organizer activity

Isthmus-to-midbrain transformation in the absence of midbrain-hindbrain organizer activity

Molecular evolution and expression of zebrafish St8SiaIII, an alpha‐2,8‐sialyltransferase involved in myotome development

Central and Peripheral Axon Branches from One Neuron Are Guided Differentially by Semaphorin3D and Transient Axonal Glycoprotein-1

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