Topographic association between active gastritis and Campylobacter pylori colonisation.

Bayerdörffer, Ekkehard; Oertel, Horst; Lehn, Norbert; Kasper, G.; Mannes, G. A.; Sauerbruch, Tilman; Stolte, Manfred

doi:10.1136/jcp.42.8.834

Cited by 186 publications

(82 citation statements)

References 17 publications

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“…However, the site-dependent colonisation observed was similar to that described for man [34]. It has been reported that differences in distribution relate to differences in local acid output in the H. felis-infected mouse model [35].…”

Section: Discussionmentioning

confidence: 53%

Experimental Helicobacter pylori gastric infection in miniature pigs

Koga¹,

Shimada²,

Satô³

et al. 2002

Journal of Medical Microbiology

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An experimental Helicobacter pylori infection in miniature pigs was developed and investigated. Eighteen miniature pigs were inoculated with an H. pylori strain that has high virulence in mice at c. 5 3 10 10 cfu. H. pylori infection in miniature pigs was achieved by the administration of agar 1% in brucella broth with fetal bovine serum 10% just before inoculation. The bacterial colonisation and distribution were analysed by mapping of viable cell counts in the stomach in pigs of three different ages. The mapping assay was achieved on post-infection day 3 for the 5-day-old and 2-week-old pigs, and between days 41 and 43 for 3-month-old pigs. The highest cell counts were observed in 5-day-old pigs, which averaged 4:9 3 10 6 cfu=g of mucosa (n 4). The bacteria were colonised mainly in the cardiac and fundus gland region in the 5-day-old and 2-week-old pigs, whereas the colonisation sites did not depend on the region in the 3-month-old pigs. Biopsy assay of the antral mucosa of a 3-month-old pig after H. pylori infection showed that this infection persisted for >22 months. Serum antibody against H. pylori was detected in the infected pigs but not in the uninfected animal. Immunostaining demonstrated the presence of bacteria on the epithelial surface of the infected pigs. A microscopic ®nding common to all the infected pigs, focal gastritis with in®ltration of lymphocytes detected on the lesser curvature of the stomach, resembled the microscopic appearance in H. pylori-infected human patients. These results suggest that miniature pigs might be a suitable model for studying H. pylori infection.

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Section: Discussionmentioning

confidence: 53%

Experimental Helicobacter pylori gastric infection in miniature pigs

Koga¹,

Shimada²,

Satô³

et al. 2002

Journal of Medical Microbiology

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“…4: SS1 predominated in antrum mucosal DNA (B), but not among single-colony isolates (A)]. Such differences may stem from use of different halves of the stomach in these two tests and the patchiness of many H. pylori infections (Bayerdorffer et al, 1989;Anonymous, 1986).…”

Section: Strain Distribution Does Not Depend On Order Of Inoculationmentioning

confidence: 99%

Helicobacter pylori tissue tropism: mouse-colonizing strains can target different gastric niches

et al. 2003

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Studies with the mouse-adapted Helicobacter pylori strain SS1 had supported an idea that infections by this pathogen start in the gastric antrum and spread to the corpus after extensive mucosal damage. This paper shows that the unrelated strain X47 colonizes the corpus preferentially. Differences between strains in preferred gastric region were detected by co-inoculating mice with a mixture of SS1 and X47, and genotyping H. pylori recovered after 2-8 weeks of infection by vacA s allele PCR and RAPD fingerprinting. Mixed infections were found in each of 59 co-inoculated young C57BL/6J mice. On average, however, SS1 was fourfold more abundant than X47 in the antrum and X47 was threefold more abundant than SS1 in the corpus. Similar results were obtained in mice inoculated first with one strain and then the other strain 2 weeks later. SS1 was even more abundant in the antrum of elderly (>1 year old) mice (97 % of isolates). Qualitatively similar SS1 and X47 tissue distributions were seen using unrelated mouse lines (AKR/J, A/J, DBA/2J, BALB/cJ, LG/J, SM/J), but with significantly different SS1 : X47 ratios in some cases. These results suggest the existence of at least two distinct gastric niches whose characteristics may be affected by host genotype and age (physiology), and indicate that strains differ in how effectively they colonize each niche. Differences among gastric regions and the mixed infections that these allow may contribute to H. pylori diversity and genome evolution.

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“…1 Nevertheless, the sensitivity of biopsy-based tests and culture is significantly influenced by the density, viability and distribution of bacteria in the gastric mucosa. 6 In certain circumstances, e.g. short time after use of antibiotics, recent or ongoing use of proton pump inhibitors (PPI), and in patients with gastric mucosal atrophy, biopsy based methods may fail due to biopsy sampling error.…”

Section: Introductionmentioning

confidence: 99%

Polymerase chain reaction versus culture in the diagnosis of Helicobacter pylori infection

Johannessen

Bergh

Jianu

et al. 2013

Gastroenterol Insights

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In the management of Helicobacter pyloriinduced gastroduodenal disease, a pilot study at our hospital (St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway) revealed that culture often seemed to fail compared to the polymerase chain reaction (PCR). A more thorough evaluation was therefore undertaken. We included 201 patients referred to upper gastrointestinal endoscopy in the period [2002][2003][2004]. Serology, biopsy rapid urease test, culture and PCR were performed. Conventional PCR was performed using the ureC, vacA and cagA genes, and real-time PCR for ureC. A diagnostic standard was defined on the basis of all four tests, and all four tests were then compared to this standard. One hundred eleven patients were deemed H. pylori-positive by the defined diagnostic standard, and 90 were labeled negative. Compared to this standard, culture showed a sensitivity of 87.4%, which was significantly lower than PCR at 99.1% (P<0.001). Culture showed a perfect specificity of 100%, which was significantly better than PCR at 97.8%. ureC was the gene with the best sensitivity (94.6% in conventional PCR, 97.3% in real-time PCR). vacA sensitivity was 87.4%, which is significantly lower than ureC (P<0.001). cagA was present in 37.8% of our H. pylori-positive patients. By real-time PCR a significantly lower cycle threshold was observed in antral biopsies than in corpal biopsies, indicating a higher H. pylori DNA template concentration in antral biopsies. PCR-testing for H. pylori is faster and significantly more sensitive than culture. Culture on the other hand was significantly more specific than PCR in our hand.

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Topographic association between active gastritis and Campylobacter pylori colonisation.

Cited by 186 publications

References 17 publications

Experimental Helicobacter pylori gastric infection in miniature pigs

Experimental Helicobacter pylori gastric infection in miniature pigs

Helicobacter pylori tissue tropism: mouse-colonizing strains can target different gastric niches

Polymerase chain reaction versus culture in the diagnosis of Helicobacter pylori infection

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