Topochemistry, optical anisotropy and FT-IR microspectroscopy of the cocoon of Lithurgus chrysurus (Hymenoptera, Megachilidae)

Mello, Maria Luiza S.; Anjos, Eli Heber Martins dos; Vidal, Benedicto de Campos; Rozen, Jerome G.

doi:10.1016/j.micron.2016.09.005

Cited by 4 publications

(5 citation statements)

References 36 publications

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“…No evidence of overall proteins, glycoproteins, and AGAG or proteoglycans was found when using the Ponceau SS dye, the PAS reaction, and the toluidine blue tests, respectively. Under the same Ponceau SS staining conditions used here, proteins stain in a multitude of materials, from vertebrate collagens and noncollagenic proteins of the extracellular matrix to insect cocoon proteins (Vidal & Mello, 2005;Mello et al, 2016). Clear images of Ponceau SS-stained proteins have been recently reported in cocoons of the bee Lithurgus chrysurus (Mello et al, 2016).…”

Section: Discussionmentioning

confidence: 79%

“…Under the same Ponceau SS staining conditions used here, proteins stain in a multitude of materials, from vertebrate collagens and noncollagenic proteins of the extracellular matrix to insect cocoon proteins (Vidal & Mello, 2005;Mello et al, 2016). Clear images of Ponceau SS-stained proteins have been recently reported in cocoons of the bee Lithurgus chrysurus (Mello et al, 2016). Ponceau SS is also widely used in electrophoresis studies as a dye of choice for locating polypeptides on Western blots for blot-sequencing.…”

Section: Discussionmentioning

confidence: 91%

“…Regarding PAS reaction, it is a classic assay for glycoproteins (Lison, 1960; Kiernan, 2008). Toluidine blue staining usually reveals proteoglycans in extracellular matrices, mucins, bee salivary and Malpighian tubule secretions, and cocoon walls of bumblebees and solitary bees (Mello & Vidal, 1971, 1979, 2003; Vidal & Mello, 1974, 2016; Mello, 1982; Mello & Garófalo, 1986; Mello et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Considering that the composition and the structural organization of these covering films have not yet been determined, in the present study these materials were analyzed using topochemical methods, fluorescence and high-performance polarization microscopy, and infrared (IR) microspectroscopy. These procedures have recently been seen to be useful for understanding the nature and organization of several biopolymers, including silk elements in bee cocoon walls (Mello et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Polarization Microscopy and Infrared Microspectroscopy of Integument Coverings of Diapausing Larvae in Two Distantly Related Nonsocial Bees

2018

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The larvae of the two distantly related nonsocial bees Ericrocis lata (Apidae) and Hesperapis (Carinapis) rhodocerata (Melittidae), which develop mostly under arid desert areas of North America, and that differ in that they either spin (E. lata) or do not spin (H. rhodocerata) protective cocoons before entering diapause, produce transparent films that cover the larval integument. To understand the nature of these films, their responses to topochemical tests and their characteristics when examined with fluorescence and high-performance polarization microscopy and microspectroscopy were studied. A positive staining by Sudan black B, birefringence of negative sign, and a Fourier transform-infrared (FT-IR) spectrum typical of lipids were detected for the integument covering of both species. The FT-IR signature, particularly, suggests a wax chemical composition for these lipid coverings, resembling the waxes that are used as construction materials in the honey cells produced by social bees. Considering the arid environmental conditions under which these larvae develop, we hypothesize that their covering films may have evolved as protection against water depletion. This hypothesis seems especially appropriate for H. rhodocerata larvae, which are capable of undergoing a long diapause period in the absence of a protective cocoon.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polarization Microscopy and Infrared Microspectroscopy of Integument Coverings of Diapausing Larvae in Two Distantly Related Nonsocial Bees

2018

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“…In particular, microscopic infrared spectroscopy, which allows measurement of the infrared absorption spectrum of microscopic areas, has recently attracted attention [16][17][18][19][20] . Generally, the histology of lesion sample slices are observed using a microscope; however, the infrared absorption spectrum of microscopic areas observed can be obtained without staining tissue slices when the procedure is conducted using microscopic infrared spectroscopy [21][22][23] . In this study, we focused on the infrared spectra of collagen and proteoglycans produced by osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Demineralized Mammalian Bone Tissue by Fourier Transform Infrared Microscopy

Yamaguchi

Yamamoto

Tsujigiwa

2018

J. Hard Tissue Biology.

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The amounts and properties of cartilage matrix components such as collagen and proteoglycans change in joint and bone related diseases, and spectroscopic studies of these components are important for developing new diagnostic and therapeutic methods. Infrared spectroscopy provides information on chromophores and vibrations related to characteristic molecules present in cells and tissues. In addition, infrared spectroscopy is a non-invasive and useful spectroscopy based analytical technique that does not use antibody labeling. In this study, we used decalcifi ed unstained specimens of canine mandibular bone and rat and rabbit tibia. The specimens were subjected to microscopic infrared spectroscopy, and analyzed by determining the infrared spectrum, with a focus on fi ber proteins (collagen) and carbohydrates (proteoglycans) present in the specimens. Normally, a large signal originating from the PO stretching mode of phosphate (PO 4 3-) is observed during measurements of the infrared absorption spectrum of bone sections; however, the above mentioned signal was not observed in our study as the samples used in our study were decalcifi ed. Instead, we observed signals from proteoglycans that are usually masked by signals originating from PO stretching and are diffi cult to observe. Measurements were performed on the same types of bone sections, and the results showed that the infrared spectrum varied greatly depending on the portion subjected to the measurements. However, in contrast, the results of the measurements conducted on the cartilage regions of rat and rabbit tibia coincided in terms of the amide I, amide II, and amide III bands, as well as the signal intensity ratio of the proteoglycan signal. Our fi ndings indicated that infrared spectrum patterns allowed the identifi cation of cartilaginous tissues in bone sections. By using this characteristic signal as a marker and carrying out two dimensional mapping on the cartilage part, for example, it is suggested that it can be applied to analysis of collagen distribution in demineralized fossil samples.

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Sodium valproate (VPA) interactions with DNA and histones

Vidal

Mello

2020

International Journal of Biological Macromolecules

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Topochemistry, optical anisotropy and FT-IR microspectroscopy of the cocoon of Lithurgus chrysurus (Hymenoptera, Megachilidae)

Cited by 4 publications

References 36 publications

Polarization Microscopy and Infrared Microspectroscopy of Integument Coverings of Diapausing Larvae in Two Distantly Related Nonsocial Bees

Polarization Microscopy and Infrared Microspectroscopy of Integument Coverings of Diapausing Larvae in Two Distantly Related Nonsocial Bees

Analysis of Demineralized Mammalian Bone Tissue by Fourier Transform Infrared Microscopy

Sodium valproate (VPA) interactions with DNA and histones

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