TOPICALLY-USED GENTAMICIN ATTACHED TO NANOFIBRE MDOCTM COMPARED WITH GARAMYCIN SCHWAMM^® IN AN ACUTE WOUND INFECTION MODEL. AN EXPERIMENTAL STUDY.

Abstract

SummaryThe aim of this study was to examine the effect of topically-used gentamicin attached to a biodegradable carrier, formed by micro-dispersed oxidised cellulose (MDOC) in nanofibre form, in acute wound infection treatment and to compare it with Garamycin Schwamm ® . Twelve domestic swines were used in a model of a full-thickness infected dermal wound. The effectiveness of both materials in wound infections caused by Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli was tested. The effectiveness of both gentamicin with MDOC and Garamycin Schwamm ® was comparable in Pseudomonas aeruginosa and Escherichia coli infections according to microbiological cultures findings. With Staphylococcus aureus infections there was a higher percent of negative cultures when MDOC with gentamicin was administered, but without statistical significance (p=0.069). When macroscopically assesed, 100 % of infected wounds treated by gentamicin attached to MDOC were without signs of local infection compared to only 16.7 % when Garamycin Schwamm ® was used and this was statistically significant (p<0.01). For statistical analysis we used a Fisher´s exact test. When combined with a nanofibre MDOC carrier, topically-used gentamicin seems to be rendered more for treatment of full-thickness skin infections. The resulting good haemostatic effect of MDOC was observed.