Topically Applied Luteolin /Quercetin-Capped Silver Nanoparticle Ointment as Antileishmanial Composite: Acceleration Wound Healing in BALB/c Mice

Abstract

Cutaneous leishmaniasis is a major health problem in several parts of the world. However, existing drugs have various therapeutic effects on different Leishmania species and also cause many side effects. This study focuses on the use of the aqueous extract of quercetin in producing silver nanoparticles for the experimental treatment of cutaneous leishmaniasis in laboratory mice. Silver nanoparticles (AgNPs) were synthesized using quercetin and characterized by UV-Vis spectroscopy, DLS, FTIR, and FESEM. We investigated the antileishmanial activity of green synthesized AgNPs in vitro and in vivo. Synthesized AgNPs from quercetin macromolecules showed promising antileishmanial activity at an IC50 of 125 μg/ml against promastigotes in vitro. In the in vivo study, L. major-infected BALB/c mice were treated topically with luteolin/quercetin-capped silver nanoparticle (AgNPQct) ointment for 21 consecutive days. As a result, this ointment reduced the inflammatory response compared to the untreated group ( p < 0.05). The size of lesions in the luteolin/AgNPQct group showed a slight increase compared to other groups ( p < 0.01). Biogenic silver nanoparticles along with the luteolin macromolecule can be an effective and reliable candidate for the design of antileishmanial drugs in the future.

Quercetin as a Promising Antiprotozoan Phytochemical: Current Knowledge and Future Research Avenues

“…Another important in vitro finding is that Que has greater activity against L. major than Glucantime® [ 38 , 41 ]. In another study, Que-capped silver nanoparticles exhibited substantially higher leishmanicidal activities against L. major promastigotes than Que alone or Glucantime® [ 42 ]. Undoubtedly, nanocarriers hold great potential for improving the antileishmanial activity of Que.…”

“…Reductions in both lesion size and inflammatory responses, along with acceleration of wound healing, were observed in Que-treated animals infected with L. major [40][41][42]94]. In one study, the abundance of apoptotic neutrophils containing apoptotic amastigotes in Que-treated mice was noticed [10], suggesting that exposure to Que could markedly abrogate L. major-induced apoptosis delay.…”

Biofabrication of nanoparticles: sources, synthesis, and biomedical applications

“…These electrons can scan things in a zig-zag manner. An emission source frees electrons propelled by a high electric field gradient ( Ebrahimzadeh et al, 2023 ). Approximately all of the NPs in the FESEM micrograph should be perfect round spheres, which seems to be a frequently noticed characteristic of biosynthesized NPs ( Nile et al, 2023 ).…”

“…Using Brookhaven Zeta Plus and dynamic light scattering (DLS) measurements, the hydrodynamic size of the phytofabricated NPs and PDI were assessed using nanoparticle-tracking analysis. The zeta potential was used to calculate the net surface charge ( Ebrahimzadeh et al, 2023 ).…”

Exploring the Impact of Leishmania Major on Mesenchymal Stem Cells: Evaluating Differentiation, and Immunomodulatory Function

“…Despite efforts to control and treat it, leishmaniasis remains a problem due to limited treatment options and drug resistance. 14 Recently, there has been interest in MSC therapy as a potential treatment for leishmaniasis. MSCs can migrate to affected tissues, interact with immune cells, and modulate the immune response to reduce inflammation and tissue damage.…”