Topical spray of Wharton's jelly mesenchymal stem cells derived from umbilical cord accelerates diabetic wound healing

Abstract

Background and Aim: Cell-based therapy utilizing mesenchymal stem cells (MSCs) is currently being investigated as a therapeutic agent for chronic wounds. There is no evidence regarding effectiveness of the spray and local transfer of this cellular product in diabetic wound healing. Accordingly, the present study, using clinical, pathological and biometric parameters, investigated the effectiveness of the spray of these cells in the healing of diabetic wounds in rats.Methods: Three days after the induction of diabetes (50 mg/kg single dose of streptozotocin) a circular excision was created on the back of rats. Diabetic rats were divided into two groups (n = 21): Control and WJ-MSCs group. Sampling of the studied groups was performed on Days 7, 14, and 21 after wounding. Histological, ultrasound imaging of dermis and epidermis in the wound area for thickness and density measurement and skin elasticity were evaluated.Results: Our results on Days 7, 14, and 21 after wounding showed that the wound closure, thickness, and density of new epidermis and dermis, as well as skin elasticity

Perinatal Stem Cell Therapy to Treat Type 1 Diabetes Mellitus: A Never-Say-Die Story of Differentiation and Immunomodulation

“…The change of c-peptide was significantly higher in the MSC-treated group than in the control group in adult-onset, but not different between groups among juvenile-onset T1DM [ 95 ]. Very recently, it has been shown that when a suspension spray including WJ-MSCs is applied on the wound area, it accelerates healing in diabetic wounds [ 96 ].…”

“…When two histone deacetylase (HDAC) inhibitors were tested, the pre-treatment with TMP269 improved the expression of insulin, somatostatin, GLUT2, PDX-1, PAX4, and NKX6.1 pancreatic markers [ 96 ]. Beikmohammadi et al [ 105 ] transfected WJ-MSCs in order to generate hepatocyte-like cells and differentiated them by using serial exposure to different inducing material and exogenous growth factors.…”

Sprayable Bioactive Dressings for Skin Wounds: Recent Developments and Future Prospects

“…In vivo-murine model [68] Wharton's jelly mesenchymal stem cells from umbilical cord Saline solution Syringe with spray head Diabetic wounds [105] has lower rejection and modulates the inflammatory phase response. The last-mentioned feature has been considered the main factor in reducing scar formation [134].…”

Transplantation of Wharton's jelly mesenchymal stem cells encapsulated with Hydroactive® Gel promotes diabetic wound antifibrotic healing in type 2 diabetic rats

“…13,14 WJMSCs suspension spray on the wound area can facilitate wound closure, promote the regeneration of epidermis and dermis, and increase skin elasticity. 15 However, subcutaneous injections will cause secondary damage to the skin tissue at the wound, which can easily lead to inflammation. Besides, diabetic wounds are further complicated because of reduced cytokine responses, infection, poor vascularization, and delayed healing processes.…”