Topical ozonated virgin coconut oil improves wound healing and increases HSP90α, VEGF-A, EGF, bFGF and CD34 in diabetic ulcer mouse model of wound healing

Yuniati, Renni; Subchan, Prasetyowati; Riawan, Wibi; Khrisna, Matthew Brian; Restiwijaya, M.; Dyan, Niken Safitri; Nur, Muhammad

doi:10.12688/f1000research.22525.1

Cited by 3 publications

(4 citation statements)

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“…They found that the wound closure rate in the VCO group was higher on all days compared to diabetic nontreated rats, and VCO was found to be better than silver sulfadiazine cream in the healing of diabetic wounds via promoting re-epithelialization and collagen synthesis, as well as increasing WCR and total protein content. A few other studies reported the effectiveness of pure VCO (liquid form) in promoting the healing process [ 45 , 46 , 47 ]. The wound healing potency of fermented virgin coconut oil was also verified against human umbilical vein endothelial (HUVEC), fibroblast (CCD-18), and retinal ganglion (RGC-5) cells, as well as a wound excision model in Sprague–Dawley rats [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Gellan Gum Hydrogels Filled Edible Oil Microemulsion for Biomedical Materials: Phase Diagram, Mechanical Behavior, and In Vivo Studies

Muktar

Bakar²,

Amin

et al. 2021

Polymers

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The demand for wound care products, especially advanced and active wound care products is huge. In this study, gellan gum (GG) and virgin coconut oil (VCO) were utilized to develop microemulsion-based hydrogel for wound dressing materials. A ternary phase diagram was constructed to obtain an optimized ratio of VCO, water, and surfactant to produce VCO microemulsion. The VCO microemulsion was incorporated into gellan gum (GG) hydrogel (GVCO) and their chemical interaction, mechanical performance, physical properties, and thermal behavior were examined. The stress-at-break (σ) and Young’s modulus (YM) of GVCO hydrogel films were increased along with thermal behavior with the inclusion of VCO microemulsion. The swelling degree of GVCO hydrogel decreased as the VCO microemulsion increased and the water vapor transmission rate of GVCO hydrogels was comparable to commercial dressing in the range of 332–391 g m−2 d−1. The qualitative antibacterial activities do not show any inhibition against Gram-negative (Escherichia coli and Klebsiella pneumoniae) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) bacteria. In vivo studies on Sprague–Dawley rats show the wound contraction of GVCO hydrogel is best (95 ± 2%) after the 14th day compared to a commercial dressing of Smith and Nephew Opsite post-op waterproof dressing, and this result is supported by the ultrasound images of wound skin and histological evaluation of the wound. The findings suggest that GVCO hydrogel has the potential to be developed as a biomedical material.

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Section: Resultsmentioning

confidence: 99%

Gellan Gum Hydrogels Filled Edible Oil Microemulsion for Biomedical Materials: Phase Diagram, Mechanical Behavior, and In Vivo Studies

Muktar

Bakar²,

Amin

et al. 2021

Polymers

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“…with O 3 therapy (Eroglu et al, 2019;Yuniati et al, 2020). Prostacyclin (PGI2) and nitric oxide (NO) are potent vasodilators that play key roles in the maintenance of vascular homeostasis (Mitchell et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Endometrial angiogenesis induced by uterine insufflation with an oxygen–ozone gas mixture in mares

Ferreira

Amaral

Cardoso

et al. 2021

Reprod Domestic Animals

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Ozone (O3) therapy has been used to improve peripheral tissue oxygenation in humans and domestic animals. The goal of the present study was to characterize histological changes in the endometria of healthy equines following tissue exposure to gas mixtures enriched with different concentrations of O3. Cycling mares without endometrial degeneration were divided into three groups according to treatment (n = 9 mares/group). The uteri from the O3, ½O3 and control groups were insufflated for 3 min with gas containing 42, 21 and 0 μg O3 ml‐1, respectively. Treatments were performed every three days from D0 to D6. Endometrial samples were collected immediately before the first treatment and 24 hr after the last treatment. The following nine histological parameters were evaluated: (i) the number of endometrial blood vessels, (ii) endometrial vascular degree (EVD), (iii) increase rate of blood vessels, (iv) increase rate of EVD, (v) glandular total area, (vi) glandular lumen area, (vii) intraglandular secretion area, (viii) glandular epithelial height and (ix) luminal epithelial height. In the O3 group, a positive effect from treatment (p < .01) was detected for all vascular parameters (i, ii, iii and iv), glandular total area, intraglandular secretion area and glandular epithelial height. Compared to the control group, the ½O3 group had greater (p < .01) EVD (84.1 ± 12%) and a higher increase rate of blood vessels (151.9 ± 47.1%). Uterine insufflation with low or intermediate concentrations of the O2–O3 gas mixture induced endometrial angiogenesis. Morphometry, but not morphology, of the endometrial glands was affected by local O3 therapy. These findings would be of great significance for the development of new therapies for infertility in mares.

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“…Medical ozone was generated by using a DDBD plasma generator which consists of two Pyrex glass barriers separated by 3 mm from each other (13,14) . This type of DDBD is known to generate high ozone purity, as the barriers keep the electrodes from corrosion, preventing the produced ozone from contamination (14) .…”

Section: B Medical Ozone Generatormentioning

confidence: 99%

“…(4,6,(8)(9)(10) Ozone bagging therapy for diabetic foot ulcers is beneficial as an anti-microbial effect, increasing glycemic control, increasing insulin sensitivity, preventing oxidative stress, increasing fibroblast proliferation, and increasing the release of growth factors such as PDGF, TGF-β, and VEGF which make the wound healing process faster and more effective. (10,12,13) The use of ozone bagging for diabetic foot ulcers abroad has been widely used. (6,9,10) But in Indonesia, is still not widely used, while the Medical Ozone Generator device found by researchers in Indonesia has met the standards for use as an adjuvant therapy for diabetic foot ulcers.…”

Section: Introductionmentioning

confidence: 99%

Case Study of Ozone Bagging Adjuvant Therapy in Wagner II and III Diabetic Foot Ulcers on Wound Healing Process: Three Patients Recovered Completely

Nur Fahada,

Pratamayoga,

Thohar Arifin

2023

Int J Sci Healthcare Res

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Background: Diabetic foot ulcer is a chronic complication of diabetes mellitus in the form of ulceration with or without infection. Diabetic foot ulcers that are not handled properly can cause damage to the foot that requires amputation. Wound care techniques for treating diabetic foot ulcers are starting to develop, one of which is ozone bagging therapy. This ozone bagging therapy in diabetic foot ulcers is reported to be useful as an anti-microbial effect, increasing glycemic control, increasing insulin sensitivity, preventing oxidative stress, increasing fibroblast proliferation, and increasing the release of growth factors. This case study will report 3 patients with Wagner II and III-foot ulcers who healed completely within 28 days using ozone bagging treatment as an adjuvant. Methods: 3 patients with diabetic foot ulcers Wagner II and III received ozone bagging therapy every 4 days for 7 sessions (a total of 28 days) as an additional therapy besides treating moist gauze using NaCl. This study used a Medical Ozone Generator. The dose of ozone used with medical oxygen is 1 lpm, flow 0.6, in the first session "high dose" with an output dose of 80-100 mg/l and in the 2nd-7th session with a low dose (25-35 mg/l). Macroscopic assessment of the wound using the Wagner scale and the progress of each treatment session is documented in photographs. Results: On macroscopic measurements, the process of wound healing leading to wound closure was better in the treatment group and was taken as a case for 3 patients who recovered completely. It resulted in 3 patients who recovered completely with a measurement of the Meggitt-Wagner scale of 0.26. Conclusion: For these recovered patients, there was a decrease in VEGF, TGF-β, and PDGF levels. The VEGF, TGF-β, and PDGF levels tested were VEGF, TGF-β, and PDGF levels in wound exudate by comparing them on the 3rd day. 1st and 12th day. Supported by macroscopic data showing that the wound healing process was better in the group that received ozone bagging therapy as an adjuvant. From this study it was found that the trend of decreasing levels of VEGF, TGF-β, and PDGF in wound exudate occurred because the inflammatory and proliferative processes in the treatment group lasted more faster than the control group. Keywords: ozone bagging therapy, diabetic ulcers, wound exudate, growth factors, VEGF, TGF-β, and PDGF, Meggitt-Wagner scale.

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Topical ozonated virgin coconut oil improves wound healing and increases HSP90α, VEGF-A, EGF, bFGF and CD34 in diabetic ulcer mouse model of wound healing

Cited by 3 publications

References 60 publications

Gellan Gum Hydrogels Filled Edible Oil Microemulsion for Biomedical Materials: Phase Diagram, Mechanical Behavior, and In Vivo Studies

Gellan Gum Hydrogels Filled Edible Oil Microemulsion for Biomedical Materials: Phase Diagram, Mechanical Behavior, and In Vivo Studies

Endometrial angiogenesis induced by uterine insufflation with an oxygen–ozone gas mixture in mares

Case Study of Ozone Bagging Adjuvant Therapy in Wagner II and III Diabetic Foot Ulcers on Wound Healing Process: Three Patients Recovered Completely

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