Topical methyl-aminolevulinate photodynamic therapy using red light-emitting diode light for treatment of multiple actinic keratoses: A randomized, double-blind, placebo-controlled study

Pariser, David M.; Loss, Robert W.; Jarratt, Michael; Abramovits, William; Spencer, James M.; Geronemus, Roy G.; Bailin, Philip L.; Bruce, Suzanne

doi:10.1016/j.jaad.2008.05.031

Cited by 91 publications

(63 citation statements)

References 23 publications

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“…It is a precursor of tetrapyrroles and a key intermediate in regulating tetrapyrrole biosynthesis (Sasaki et al, 2002). ALA has been used in a number of applications, i.e., as a new kind of green pesticide in agriculture and as a photodynamic medicine for photodynamic diagnosis and therapy of cancer (Lang et al, 2001;Braathen et al, 2007;Berkovitch et al, 2008;Pariser et al, 2008;Zaak et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

High-level soluble expression of the hemA gene from Rhodobacter capsulatus and comparative study of its enzymatic properties

Lou

Zhu

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:The Rhodobacter capsulatus hemA gene, which encodes 5-aminolevulinic acid synthase (ALAS), was expressed in Escherichia coli Rosetta (DE3) and the enzymatic properties of the purified recombinant ALAS (RC-ALAS) were studied. Compared with ALASs encoded by hemA genes from Agrobacterium radiobacter (AR-ALAS) and Rhodobacter sphaeroides (RS-ALAS), the specific activity of RC-ALAS reached 198.2 U/mg, which was about 31.2% and 69.5% higher than those of AR-ALAS (151.1 U/mg) and RS-ALAS (116.9 U/mg), respectively. The optimum pH values and temperatures of the three above mentioned enzymes were all pH 7.5 and 37 °C, respectively. Moreover, RC-ALAS was more sensitive to pH, while the other two were sensitive to temperature. The effects of metals, ethylene diamine tetraacetic acid (EDTA), and sodium dodecyl sulfate (SDS) on the three ALASs were also investigated. The results indicate that they had the same effects on the activities of the three ALASs. SDS and metal ions such as Co

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Section: Introductionmentioning

confidence: 99%

High-level soluble expression of the hemA gene from Rhodobacter capsulatus and comparative study of its enzymatic properties

Lou

Zhu

et al. 2014

J. Zhejiang Univ. Sci. B

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“…Preliminary results were excellent; however, these were not sustained after 3 and 6 months. Recently, Pariser et al 7 conducted a doubleblind, randomized, placebo-controlled study of PDT with MAL for AK using a LED source; however, the parameters were also lower than those used in the present study. Cure rates obtained after three months were similar to those described in the literature with other lamps.…”

Section: Discussionmentioning

confidence: 69%

“…In dermatology and, more specifically, for the treatment of AKs, open studies using PDT with δALA and non-coherent light in patients with AKs of the face and scalp reported cure rates of 71-100% following a single application. 7,12,[17][18][19] In a study conducted by this group using PDT with δALA and a non-coherent experimental light source, clinical cure was achieved in 65.7% of AKs treated with a single application. 10 In addition, multicenter randomized studies A B…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown their efficacy in vitro and in vivo for the treatment of actinic keratoses, achieving rates similar to those found with other light sources. [7][8][9] One of these studies conducted by Juzeniene et al 8 compared two non-coherent light sources in PDT with MAL: one wide spectrum source (560-740 nm) obtained with a halogen lamp and another narrow band source (580-670 nm) obtained with a LED lamp. With respect to efficacy, in vivo findings showed that, compared to the halogen lamp, the LED lamp exerted a greater more profound effect on tissues, generated less heat and resulted in less pain when used in volunteers with healthy skin, while in animal models and cell culture it showed an efficacy similar to or better than that found with photoactivation of PpIX.…”

Section: Discussionmentioning

confidence: 99%

“…They offer a continuous dose at optimal intensity, and for this reason, interest in their use in PDT has increased since they are considered to be safer and easier to use. 7 The need to adapt previously established parameters to new light sources has motivated many publications. [8][9][10] The objective of this series of cases was to evaluate the efficacy of PDT with ‰-ALA, using a Brazilian-made LED device for the treatment of actinic keratoses keratosis (AK) of the face and upper limbs.…”

Section: Introductionmentioning

confidence: 99%

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Terapêutica fotodinâmica com ácido delta-aminolevulínico e luz de diodos em ceratoses actínicas

Ritter

Kuhl

Lenhardt

et al. 2010

An. Bras. Dermatol.

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BACKGROUND: Photodynamic therapy is a form of treatment in which a photosensitizing substance is applied to tissue and activated by a light source at a specific wavelength, thus selectively destroying cells. New light sources are being evaluated for use in the treatment of actinic keratoses. OBJECTIVES: To evaluate the efficacy of photodynamic therapy with delta-aminolevulinic acid using a light emitting diode device as a light source in the treatment of actinic keratoses of the face and upper limbs. METHODS: Eighteen patients with actinic keratoses of the face or upper limbs received an application of a 20% delta-aminolevulinic acid cream and were submitted to diode light irradiation at a wavelength of 630 nm. RESULTS: A total of 328 actinic keratoses were treated, obtaining complete cure in 210 (64.0%) after 24 weeks. Lesions situated on the back of the hands were clinically cured in 49.2% of cases compared to 81.4% in the cases of lesions in other areas. There was no record of any severe adverse effects and patient satisfaction with the results was high. CONCLUSION: Photodynamic therapy with a diode light emitting source proved effective and well-tolerated for the treatment of actinic keratoses, with results similar to those reported in the literature with other light sources. Keywords: Keratosis; Photobiology; Photochemotherapy Resumo: FUNDAMENTOS: A terapêutica fotodinâmica é técnica de tratamento em que se aplica uma substân-cia fotossensibilizante nos tecidos ativada por uma fonte de luz de comprimento de onda específico, gerando destruição celular seletiva. Estudam-se novas fontes de luz que possam ser usadas no tratamento de ceratoses actínicas. OBJETIVOS: Avaliar a efetividade da terapêutica fotodinâmica com ácido delta-aminolevulínico utilizando como fonte de luz um aparelho emissor de luz de diodos no tratamento de ceratoses actínicas de face e membros superiores. MÉTODOS: Dezoito pacientes com ceratoses actínicas na face ou membros superiores realizaram uma aplicação de creme de ácido delta-aminolevulínico a 20% e foram submetidos à exposição de luz de diodos, comprimento de onda de 630 nm. RESULTADOS: Foram tratadas 328 ceratoses actínicas, obtendo-se cura clínica completa em 210 (64,0%) após 24 semanas. Lesões do dorso das mãos apresentaram cura clínica completa em 49,2%; nas demais áreas esse valor foi de 81,4%. Não houve registro de efeitos adversos graves, e obteve-se bom grau de satisfação dos pacientes com os resultados. CONCLUSÃO: A terapêutica fotodinâmica com fonte de emissão de luz de diodos mostrou-se eficaz e bem tolerada para tratamento de ceratoses actínicas, com resultados semelhantes aos encontrados na literatura utilizando outras fontes luminosas.

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Interventions for actinic keratoses

Gupta

Paquet

Villanueva

et al. 2012

Cochrane Database of Systematic Reviews

174

239

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For individual lesions, photodynamic therapy appears more effective and has a better cosmetic outcome than cryotherapy. For field-directed treatments, diclofenac, 5-fluorouracil, imiquimod, and ingenol mebutate had similar efficacy, but their associated adverse events and cosmetic outcomes are different. More direct comparisons between these treatments are needed to determine the best therapeutic approach.

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Topical methyl-aminolevulinate photodynamic therapy using red light-emitting diode light for treatment of multiple actinic keratoses: A randomized, double-blind, placebo-controlled study

Cited by 91 publications

References 23 publications

High-level soluble expression of the hemA gene from Rhodobacter capsulatus and comparative study of its enzymatic properties

High-level soluble expression of the hemA gene from Rhodobacter capsulatus and comparative study of its enzymatic properties

Terapêutica fotodinâmica com ácido delta-aminolevulínico e luz de diodos em ceratoses actínicas

Interventions for actinic keratoses

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