Topical Fibronectin Improves Wound Healing of Irradiated Skin

Johnson, M. P.; Pang, Brandon; Gardner, Daniel J.; Niknam-Benia, Solmaz; Soundarajan, Vinaya; Bramos, Athanasios; Perrault, David; Banks, Kian C.; Lee, Gene K.; Baker, Regina Y.; Kim, Gene; Lee, Sun-Ju; Chai, Yang; Chen, Mei; Li, Wei; Kwong, Lawrence N.; Hong, Young Kwon; Wong, Alex K.

doi:10.1038/s41598-017-03614-y

Cited by 42 publications

(45 citation statements)

References 39 publications

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“…For instance, human fetus [ [70] , [71] , [72] ] and invertebrate animals newts [ 73 ] heal wounds much faster because their provision matrices contain much more fibronectins that allow cells to migrate and proliferate faster. The topical application of pure soluble fibronectins enhances normal and diabetic wound healing in animals [ 70 , [74] , [75] , [76] , [77] , [78] ]. These soluble fibronectins aggregate or attach to surrounding tissue, but do not form a 3D matrix in the wound bed.…”

Section: Discussionmentioning

confidence: 99%

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Jara

Wang

Prado

et al. 2020

Bioactive Materials

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Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1), and 10% has a heterodimeric pair of γ and more acidic γ′ chains (γγ′F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ′F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were decorated with pFN nanoclusters (termed γγ′F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of “pFN droplets” (termed γγF1+pFN fibrin). The γγ′F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ′F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ′F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ′F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN presentation on a fibrin matrix promotes wound healing.

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Section: Discussionmentioning

confidence: 99%

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Jara

Wang

Prado

et al. 2020

Bioactive Materials

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“…During normal wound healing, fibronectin can induce the regeneration of epithelial tissue by recognizing the RGD sequence on the epithelial cell integrin receptor α5β1 [32, 33]. However, the absence of fibronectin expression in irradiated skin leads to skin wounds that do not heal properly [34]. It was also reported that fibronectin is regulated by TGF-β1 in a c-Jun N-terminal kinase-dependent and Smad4-independent manner [35].…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived stem cells prevent the onset of bisphosphonate-related osteonecrosis of the jaw through transforming growth factor β-1-mediated gingival wound healing

Zang

Zhao

et al. 2019

Stem Cell Res Ther

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Background Due to its complex pathogenesis and low clinical cure rate, bisphosphonate-related osteonecrosis of the jaw (BRONJ) poses a substantial challenge for oral and maxillofacial surgeons. Therefore, the treatment of BRONJ should focus on prevention. In clinical studies, primary wound closure can significantly reduce the incidence of BRONJ. Whether local stem cell transplantation can promote primary gingival healing in patients with a medication history and prevent BRONJ has not been reported. Methods In this study, animals were divided into a healthy group (non-drug treatment), a BP group, a hydroxyapatite (HA) group, and an adipose-derived stem cell (ADSC) group. All groups except the healthy group were treated with BPs and immunosuppressive drugs once per week for 8 weeks, simulating clinical use for the treatment of cancer patients with bone metastasis, to induce BRONJ-like animals. After the sixth drug treatment, the bilateral premolars were extracted in all groups. In contrast to the healthy and BP groups, the extraction sockets in the HA and ADSC groups were filled with HA or HA + ADSCs simultaneously post extraction to observe the preventive effect of ADSCs on the occurrence of BRONJ. At 2 and 8 weeks post extraction, animals from all groups were sacrificed. Results At 8 weeks post transplantation, ADSCs prevented the occurrence of BRONJ, mainly through accelerating healing of the gingival epithelium at 2 weeks post extraction. We also found that ADSCs could upregulate the expression of transforming growth factor β1 (TGF-β1) and fibronectin in tissue from animals with a medication history by accelerating gingival healing of the extraction socket. A rescue assay further demonstrated that TGF-β1 and fibronectin expression decreased in TGF-β1-deficient ADSC-treated animals, which partially abolished the preventive effect of ADSCs on the onset of BRONJ. Conclusion ADSCs prevent the onset of BRONJ, mainly by upregulating the expression of TGF-β1 and fibronectin to promote primary gingival healing, ultimately leading to bone regeneration in the tooth extraction socket. Our new findings provide a novel stem cell treatment for the prevention of BRONJ. Electronic supplementary material The online version of this article (10.1186/s13287-019-1277-y) contains supplementary material, which is available to authorized users.

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“…54 FINC is a glycoprotein involved in many cellular mechanisms such as cell growth and migration, [55][56][57][58][59] and like collagen, contains a number of binding sites for growth factors, including fibroblast growth factor, vascular endothelial growth factor, and platelet-derived growth factor, which have been shown to promote wound healing. [60][61][62] With regard to lipase inhibitor activity, we found more abundance of APOA1 in PU compared with control tissue. APOA1 is the major structural component of high-density lipoproteins (HDL), and has anti-inflammatory and immunomodulatory functions in addition to endothelial protective properties.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Proteomic Profiling of Pressure Ulcers in Patients with Spinal Cord Injury Identifies a Specific Protein Pattern of Pathology

Baldán-Martín

Martín-Rojas

Corbacho-Alonso

et al. 2020

Advances in Wound Care

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Objective: Severe pressure ulcers (PUs) do not respond to conservative wound therapy and need surgical repair. To better understand the pathogenesis and to advance on new therapeutic options, we focused on the proteomic analysis of PU, which offers substantial opportunities to identify significant changes in protein abundance during the course of PU formation in an unbiased manner. Approach: To better define the protein pattern of this pathology, we performed a proteomic approach in which we compare severe PU tissue from spinal cord injury (SCI) patients with control tissue from the same patients. Results: We found 76 proteins with difference in abundance. Of these, 10 proteins were verified as proteins that define the pathology: antithrombin-III, alpha-1-antitrypsin, kininogen-1, alpha-2-macroglobulin, fibronectin, apolipoprotein A-I, collagen alpha-1 (XII) chain, haptoglobin, apolipoprotein B-100, and complement factor B. Innovation: This is the first study to analyze differential abundance protein of PU tissue from SCI patients using high-throughput protein identification and quantification by tandem mass tags followed by liquid chromatography tandem mass spectrometry. Conclusion: Differential abundance proteins are mainly involved in tissue regeneration. These proteins might be considered as future therapeutic options to enhance the physiological response and permit cellular repair of damaged tissue.

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Topical Fibronectin Improves Wound Healing of Irradiated Skin

Cited by 42 publications

References 39 publications

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Adipose-derived stem cells prevent the onset of bisphosphonate-related osteonecrosis of the jaw through transforming growth factor β-1-mediated gingival wound healing

Comprehensive Proteomic Profiling of Pressure Ulcers in Patients with Spinal Cord Injury Identifies a Specific Protein Pattern of Pathology

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