Topical Application of Yeast Extract Accelerates the Wound Healing of Diabetic Mice

Crowe, Maria J.; McNeill, Redmond B.; Schlemm, Donna J.; Greenhalgh, David G.; Keller, Stephen

doi:10.1097/00004630-199903000-00032

Cited by 15 publications

(13 citation statements)

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“…the proteostasis network) remain largely elusive. In few previous studies it was found that extracts from Saccharomyces cerevisiae fermentation exert wound healing properties [16–18] . These properties were attributed to improved collagen synthesis [16,18] and to increased cellular oxygen consumption [19,20] .…”

Section: Introductionmentioning

confidence: 99%

Hexapeptide-11 is a novel modulator of the proteostasis network in human diploid fibroblasts

Sklirou

Ralli²,

Domı́nguez

et al. 2015

Redox Biology

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Despite the fact that several natural products (e.g. crude extracts or purified compounds) have been found to activate cell antioxidant responses and/or delay cellular senescence the effect(s) of small peptides on cell viability and/or modulation of protective mechanisms (e.g. the proteostasis network) remain largely elusive. We have thus studied a hexapeptide (Hexapeptide-11) of structure Phe–Val–Ala–Pro–Phe–Pro (FVAPFP) originally isolated from yeast extracts and later synthesized by solid state synthesis to high purity. We show herein that Hexapeptide-11 exhibits no significant toxicity in normal human diploid lung or skin fibroblasts. Exposure of fibroblasts to Hexapeptide-11 promoted dose and time-dependent activation of proteasome, autophagy, chaperones and antioxidant responses related genes. Moreover, it promoted increased nuclear accumulation of Nrf2; higher expression levels of proteasomal protein subunits and increased proteasome peptidase activities. In line with these findings we noted that Hexapeptide-11 conferred significant protection in fibroblasts against oxidative-stress-mediated premature cellular senescence, while at in vivo skin deformation assays in human subjects it improved skin elasticity. Finally, Hexapeptide-11 was found to induce the activity of extracellular MMPs and it also suppressed cell migration. Our presented findings indicate that Hexapeptide-11 is a promising anti-ageing agent.

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Section: Introductionmentioning

confidence: 99%

Hexapeptide-11 is a novel modulator of the proteostasis network in human diploid fibroblasts

Sklirou

Ralli²,

Domı́nguez

et al. 2015

Redox Biology

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show abstract

“…Recent studies have revealed that tight junctions (TJs) are located in a narrow zone of the stratum granulosum, comprising of peripheral and integral proteins that are necessary for connecting neighboring cells and providing Up-Regulation of Tight Junction-Related Proteins And Increase of Human Epidermal Keratinocytes Barrier Function 16 by Saccharomycosis Ferment Filtrate barrier functions [4][5][6][7]. TJs consist of transmembrane proteins including occludins, claudins, and junctional adhesion molecules (JAM), and of plaque proteins, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…The term Live yeast cell derivative (LYCD) invented by Kaplan et al in 1984 [13] was indeed a synonyms of the skin respiratory factor. LYCD was alcoholic extract from yeast (Saccharomyces cerevisiae), and capable of promoting wound healing, accelerating angiogenesis, reducing the symptoms of burns and cutting wounds, and stimulating epithelization as well as collagen synthesis [12][13][14][15][16]. Saccharomycopsis is a family of yeast which has been used to help fermentation of traditional beverages.…”

Section: Introductionmentioning

confidence: 99%

Up-regulation of tight junction-related proteins and increase of human epidermal keratinocytes barrier function by Saccharomycosis ferment filtrate

Pang¹,

Wong²,

Hakozaki³

et al. 2011

JCDSA

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Saccharomycopsis ferment filtrate (SFF), mainly used in skin care products, has been reported to inhibit inflammatory nitric oxide production and prevent epidermal damage. However, the effects of SFF on epidermal keratinocytes have not yet been explored. We investigated the effects of SFF on skin barrier function using human primary epidermal keratinocytes. Cell viability was determined by MTT assay. The mRNA and protein expression levels of tight junction proteins (claudin-1, -3, -4, occludin, ZO-1) were analyzed by RT-PCR and Western blotting, respectively. The effect of SFF on the barrier formation of epidermal keratinocytes was measured by transepithelial electrical resistance (TER). Rescue of cell death from H2O2 treatment was evaluated by annexin V staining. SFF, at concentrations that did not cause significant change of cell viability, induced dose-dependent cell-cell adhesion and formation of an organized monolayer structure. Pretreatment of keratinocytes with EGTA, a Ca2+ chelator, did not inhibit the cell-cell adhesion of keratinocytes by SFF, indicating a Ca2+-independent mechanism. The mRNA and protein levels of claudin-1 in keratinocytes were up-regulated by SFF treatment in a dose-dependent manner. The expressions of other tight junctions (TJs) including claudin-3 & 4, occludin and ZO-1 were also similarly increased in SFF-treated epidermal keratinocytes. The promoting effect of SFF on the barrier function of epidermal keratinocytes was further confirmed by the increased TER value in SFF-treated epidermal keratinocytes. Annexin V staining confirmed that SFF markedly decreased the number of dead cells resulted from H2O2 injury. Taken together, our results provided the first evidence that SFF enhanced keratinocytes barrier function by increasing the expression of TJs and TER

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“…Saccharomycopsis Ferment Filtrate (SFF), a kind of yeast extracts, was derived from filtration of saccharomycopsis fermented medium. Wildiers [5] and Crowe et al [6] proposed that the alcoholextract isolated from yeast culture contains some factors to promote the growth of yeast as well as animal cells. In addition, SFF has been used extensively in cosmetics for whitening and anti-aging.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, SFF has been used extensively in cosmetics for whitening and anti-aging. Reduction of the symptoms of burns, cutting wounds, and hemorrhoids by SFF also have been proposed [6][7][8][9]. Although SFF has been commonly used in health care, the supporting scientific evidences are still insufficient.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of inflammatory nitric oxide production and epidermis damages by Saccharomycopsis Ferment Filtrate

Tsai

Chen

Lee

et al. 2006

Journal of Dermatological Science

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Topical Application of Yeast Extract Accelerates the Wound Healing of Diabetic Mice

Cited by 15 publications

References 0 publications

Hexapeptide-11 is a novel modulator of the proteostasis network in human diploid fibroblasts

Hexapeptide-11 is a novel modulator of the proteostasis network in human diploid fibroblasts

Up-regulation of tight junction-related proteins and increase of human epidermal keratinocytes barrier function by Saccharomycosis ferment filtrate

Inhibition of inflammatory nitric oxide production and epidermis damages by Saccharomycopsis Ferment Filtrate

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