Topical application of Cinnamomum hydroethanolic extract improves wound healing by enhancing re-epithelialization and keratin biosynthesis in streptozotocin-induced diabetic mice

Abstract

Context: Cinnamomum verum J. Presl. (Lauraceae) has a high number of polyphenols with insulin-like activity, increases glucose utilization in animal muscle, and might be beneficial for diabetic patients. Objective: This study evaluated the effectiveness of an ointment prepared from Cinnamomum verum hydroethanolic extract on wound healing in diabetic mice. Materials and methods: A total of 54 male BALB/c mice were divided into three groups: (1) diabetic non-treated group mice that were treated with soft yellow paraffin, (2 and 3) mice that were treated with 5 and 10% C. verum. Two circular full-thickness excisional wounds were created in each mouse, and the trial lasted for 16 d following induction of the wound. Further evaluation was made on the wound contraction ratio, histopathology parameters and mRNA levels of cyclin D1, insulin-like growth factor 1 (IGF-1), glucose transporter-1 (GLUT-1), total antioxidant capacity, and malondialdehyde of granulation tissue contents. HPLC apparatus was utilized to identify the compounds. Results: The HPLC data for cinnamon hydroethanolic extract identified cinnamaldehyde (11.26%) and 2-hydroxyl cinnamaldehyde (6.7%) as the major components. A significant increase was observed in wound contraction ratio, fibroblast proliferation, collagen deposition, re-epithelialization and keratin biosynthesis in the C. verum-treated groups in comparison to the diabetic non-treated group (p < 0.05). The expression level of cyclin D1, IGF1, GLUT 1 and antioxidant capacity increased in the C. verum-treated groups in comparison to the diabetic non-treated group (p < 0.05). Conclusions: Topical administration of C. verum accelerated wound healing and can possibly be employed in treating the wounds of diabetic patients.

Accelerative effect of topical Zataria multiflora essential oil against infected wound model by modulating inflammation, angiogenesis, and collagen biosynthesis

“… 2016 ; Daemi et al. 2019a ). The results showed that the topical application of ZMEO increased fibroblast, which is due to its effect on IGF-1.…”

“…To assess the TTM, the collected granulation tissue sample was homogenized and the supernatant was added to Tris-EDTA as reported by Daemi et al. ( 2019a ).…”

“…The wound healing process comprises several phases, including coagulation, inflammation, epithelialization, granulation tissue formation, and tissue remodelling (Daemi et al. 2019a , 2019b ; Farahpour et al. 2020 ).…”

“…Wound infections are important challenges that cause the suffering of patients and economic losses (Vittorazzi et al 2016;Daemi et al 2019a;Farghali et al 2019). Skin is a regenerative organ which acts as a barrier between the body and the external environment (Hassan et al 2014).…”

“…Colonization of S. aureus and P. aeruginosa in the wound site postpones the wound healing process (Farahpour et al 2020;Khezri et al 2020). The wound healing process comprises several phases, including coagulation, inflammation, epithelialization, granulation tissue formation, and tissue remodelling (Daemi et al 2019a(Daemi et al , 2019bFarahpour et al 2020). Inflammatory phase occurs after activation of inflammatory chemokines (Farahpour et al 2020).…”