Topical application of glycyrrhetinic acid in the gingival sulcus inhibits attachment loss in lipopolysaccharide‐induced experimental periodontitis in rats

Takamori, A.; Yoshinaga, Y.; Ukai, Takashi; Nakamura, H.; Takamori, Yuzo; Izumi, Satoshi; Shiraishi, Chiaki; Hara, Yoshitaka

doi:10.1111/jre.12529

Cited by 6 publications

(4 citation statements)

References 28 publications

(37 reference statements)

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“…Consequently, some of the LPS may leak from the injection site. As a result, the application of LPS at a higher concentration might be implemented to compensate for the loss (19).…”

Section: Discussionmentioning

confidence: 99%

Development of experimentally-induced periodontitis in a Sprague Dawley rat model

Mustafa¹,

Ali²,

Chen³

et al. 2021

IJVS

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Periodontitis is a common inflammatory disease that leads to the degradation of periodontium and results in alveolar bone loss. The development of a suitable animal model of periodontitis is a prerequisite to understanding better the mechanisms that underly this disease. This study evaluated periodontal disease induction via retentive ligature, intragingival injection of lipopolysaccharide (LPS), and their combination in a rat model. Seventy-two Sprague Dawley rats were divided into four groups. The first group (control) did not receive any treatment. The second group underwent the application of 4/0 nylon ligature around the second maxillary molars. The third group was treated with an intragingival injection of Porphyromonas gingivalis LPS into the palatal mucosa of the second maxilla molars, and the fourth group was treated with a combination of ligature and LPS injection (ligature-LPS). Morphological changes in the gingival tissues were evaluated after 7, 14, and 30 days of treatment. Significant degenerative changes were observed in the periodontal tissues and alveolar bone in the third and fourth groups, which were evident as early as seven days. The lesions remained until 14 days and declined with time in the third and fourth groups. The changes induced by ligature and ligature-LPS were not different. Injection with LPS alone resulted in minimal increases in the Gingival and Plaque Indices. The ligature technique induced periodontal disease successfully, more effective than the injection of LPS. The combination of ligature with LPS injection added no significant effect compared to ligature alone.

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“…Consequently, some of the LPS may leak from the injection site. As a result, the application of LPS at a higher concentration might be implemented to compensate for the loss (19).…”

Section: Discussionmentioning

confidence: 99%

Development of experimentally-induced periodontitis in a Sprague Dawley rat model

Mustafa¹,

Ali²,

Chen³

et al. 2021

IJVS

View full text Add to dashboard Cite

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“…Additionally, by preventing the activation of NF-κβ, glycyrrhetinic acid can reduce the expression of IL-8, which increases vascular endothelial permeability in HMEC ( 117 ). Some researchers applied glycyrrhetinic acid locally to the gingival sulcus of the rat periodontitis model and found that glycyrrhetinic acid could effectively inhibit the formation of LPS-stimulated immune complex and inflammatory cell infiltration, and no loss of attachment was observed ( 159 ).…”

Section: Medicine Food Homology Plants Inhibit Host Inflammatory Resp...mentioning

confidence: 99%

“…In vivo test using a rat periodontitis model treated with glycyrrhizin revealed the downregulation of HMGB1, IL-6, and IL-1β in the gingival crevicular fluid and periodontal tissue (115). AGEs receptors (RAGE) in tissues and plasma of diabetic patients bound to HMGB1 are key in the association between periodontitis and diabetes (158). In diabetic mice with periodontitis, glycyrrhizic acid can suppress the production of HMGB1 and RAGE mRNA in the gingiva and serum and lessen the inflammatory response of the periodontal tissue (116).…”

Section: Saponinmentioning

confidence: 99%

“Medicine food homology” plants promote periodontal health: antimicrobial, anti-inflammatory, and inhibition of bone resorption

et al. 2023

Front. Nutr.

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“Medicine food homology” (MFH) is a term with a lengthy history. It refers to the fact that a lot of traditional natural products have both culinary and therapeutic benefits. The antibacterial, anti-inflammatory and anticancer effects of MFH plants and their secondary metabolites have been confirmed by numerous research. A bacterially generated inflammatory illness with a complicated pathophysiology, periodontitis causes the loss of the teeth’s supporting tissues. Several MFH plants have recently been shown to have the ability to prevent and treat periodontitis, which is exhibited by blocking the disease’s pathogens and the virulence factors that go along with them, lowering the host’s inflammatory reactions and halting the loss of alveolar bone. To give a theoretical foundation for the creation of functional foods, oral care products and adjuvant therapies, this review has especially explored the potential medicinal benefit of MFH plants and their secondary metabolites in the prevention and treatment of periodontitis.

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“…The main active constituent of licorice is Glycyrrhetinic acid (GA), which is obtained from the extract of licorice root. GA shows anti-inflammatory effects due to the similarity in its chemical structure with glucocorticoids by initiation of glucocorticoid receptor signaling and also by inhibiting the classical complement pathway [12]. Licorice flavonoid components include chalcones, flavones, and isoflavones, which show antimicrobial [13], antiviral [14], antiinflammatory [15], antidiabetic, antitumor, immunoregulatory [16], hepatoprotective [17] and neuroprotective activities [18].…”

Section: Introductionmentioning

confidence: 99%

Formulation and in-vitro characterization of herbal licorice gel for periodontal therapy

KHAN¹,

SHETTY²,

KHAN³

et al. 2022

jrp

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Periodontal diseases are inflammations of the periodontium caused by certain microorganisms, leading to progressive destruction of the periodontal ligament and alveolar bone. Conventional medications have limitations such as extrinsic tooth discoloration, altered taste sensation, and the development of bacterial resistance. Licorice is an herb with glycyrrhetinic acid (GA) as its main active component. GA has antimicrobial, anti-inflammatory and antioxidant activity. There is limited evidence for the preparation and in vitro evaluation of licorice extract as a topical drug in periodontology. The aim of this study was the formulation and in vitro characterization of a controlled-release gel containing licorice extract that can be used as a topical agent in periodontal therapy. An in vitro study was conducted in which an ethanolic extract of licorice was prepared. The local drug delivery gel was formulated with the specified minimum inhibitory concentration of Glycyrrhiza glabra using Carbopol 940 polymer. In vitro characterization of the formulated gel was done in terms of physical appearance, determination of pH, squirtability, drug content, gelation temperature and time, viscosity, mucoadhesive force, in vitro drug release, thermodynamic stability and X-ray diffraction analysis. The licorice gel formulation was thermodynamically stable, mucoadhesive, and sprayable. It also released the drug slowly for up to 48 hours. The incorporation of licorice extract into a polymeric gel base exhibits good physicochemical properties, adequate mucoadhesiveness, and controlled drug release. Thus, it is a potential herbal extract for local drug delivery that can be used to improve the therapeutic results of scaling and root planning.

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Topical application of glycyrrhetinic acid in the gingival sulcus inhibits attachment loss in lipopolysaccharide‐induced experimental periodontitis in rats

Abstract: In the present study, we showed that GA inhibits periodontal destruction in the rat experimental periodontitis model.

Cited by 6 publications

References 28 publications

Development of experimentally-induced periodontitis in a Sprague Dawley rat model

Development of experimentally-induced periodontitis in a Sprague Dawley rat model

“Medicine food homology” plants promote periodontal health: antimicrobial, anti-inflammatory, and inhibition of bone resorption

Formulation and in-vitro characterization of herbal licorice gel for periodontal therapy

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