Topical application of Azadirachta indica improves epidermal wound healing in hyperglycemic rats

Silva, Ana C. C.; Eugênio, Aline N.; Mariano, Samara S.; Poletti, Sofia; Gaspi, Fernanda G.; Bittencourt, João Vitor Silvano; Casagrande, Laura Roch; Esquisatto, Marcelo Augusto Marretto; Aro, Andréa Aparecida de; Andrade, Thiago Antônio Moretti de; Santos, Gláucia Maria Tech dos

doi:10.1007/s00580-021-03240-1

Cited by 9 publications

(2 citation statements)

References 45 publications

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“…Medicinal plants with AgNPs, which exhibit these pharmacological effects, also possess antioxidant activity and promote wound healing [ 32 ]. For instance, the topical application of A. indica on wounds improved wound healing through increased antioxidant (SOD, GSH) and anti-inflammatory properties in rats [ 69 ]. The leaf extracts of A. indica contain a rich amount of quenchers, redox agents, and hydrogen donors that could trigger the antioxidant enzyme activities or inactivate the free radicals [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Efficacy of Green-Synthesized Silver Nanoparticles Promotes Wound Healing in Mice

Lakkim,

Reddy,

Lekkala

et al. 2023

Pharmaceutics

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Developing an efficient and cost-effective wound-healing substance to treat wounds and regenerate skin is desperately needed in the current world. Antioxidant substances are gaining interest in wound healing, and green-synthesized silver nanoparticles have drawn considerable attention in biomedical applications due to their efficient, cost-effective, and non-toxic nature. The present study evaluated in vivo wound healing and antioxidant activities of silver nanoparticles from Azadirachta indica (AAgNPs) and Catharanthus roseus (CAgNPs) leaf extracts in BALB/c mice. We found rapid wound healing, higher collagen deposition, and increased DNA and protein content in AAgNPs- and CAgNPs (1% w/w)-treated wounds than in control and vehicle control wounds. Skin antioxidant enzyme activities (SOD, catalase, GPx, GR) were significantly (p < 0.05) increased after 11 days CAgNPs and AAgNPs treatment. Furthermore, the topical application of CAgNPs and AAgNPs tends to suppress lipid peroxidation in wounded skin samples. Histopathological images evidenced decreased scar width, epithelium restoration, fine collagen deposition, and fewer inflammatory cells in CAgNPs and AAgNPs applied wounds. In vitro, the free radical scavenging activity of CAgNPs and AAgNPs was demonstrated by DPPH and ABTS radical scavenging assays. Our findings suggest that silver nanoparticles prepared from C. roseus and A. indica leaf extracts increased antioxidant status and improved the wound-healing process in mice. Therefore, these silver nanoparticles could be potential natural antioxidants to treat wounds.

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Section: Discussionmentioning

confidence: 99%

Antioxidant Efficacy of Green-Synthesized Silver Nanoparticles Promotes Wound Healing in Mice

Lakkim,

Reddy,

Lekkala

et al. 2023

Pharmaceutics

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“…Prolonged inflammation in the wound area has been associated with delayed wound healing that can result in delayed re-epithelialization, abnormal extracellular matrix (ECM) deposition, damage to surrounding tissue, and formation of scars [7][8][9]. This prolonged inflammation has also been linked with increased oxidative stress in the wound that can result in lipid peroxidation as indicated by the presence of thiobarbituric acid reactive substances (TBARs) as well as protein oxidative damage as indicated by the presence of carbonyls and sulfhydryls [10], thus delaying wound healing [11,12]. The presence of bacterial infection in wounds has also been linked to pro-longed inflammation and increased oxidative stress [13].…”

Section: Phase 2: Inflammationmentioning

confidence: 99%

Cassia alata and Its Phytochemicals: A Promising Natural Strategy in Wound Recovery

Keng,

Lee,

Sang

et al. 2024

Sci

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Cassia alata, a traditional herb with a global presence, is renowned for its anti-inflammatory, antibacterial, and antifungal properties, making it a go-to remedy for skin ailments. While it has demonstrated wound healing capabilities in both in vitro and in vivo studies, the precise mechanisms remain elusive. This review aims to highlight its key phytochemicals, their effects, and the mechanism of action. The compounds that have been reviewed and discussed include kaempferol, apigenin, quercetin, rhein, and rutin. These polyphenols play important roles in normal and impaired wound healing processes, encompassing hemostasis, inflammation, proliferation, and tissue remodeling.

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Photobiomodulation and photodynamic therapy applied after electrocauterization for skin healing optimization in rats

Manoel

Mariano

Ramos

et al. 2022

Journal of Biophotonics

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Photobiomodulation-PBM and Photodynamic Therapy-PDT have been used to induce healing. However, the effects of these therapies on skin-lesions induced by electrocautery are unknown, aiming at more favorable clinical and esthetic results. Electrocauterization was done in 78-female Wistar-rats using a system that includes an electrocautery and red-LED. The groups were: No injury, Injury, Injury + ALA (topical 5-aminolevulinic acid application), Injury + LED and Injury + ALA + LED (topical ALA application followed by photoactivation with LED). After 2nd, 7th and 14th days post-injury, immuno-histomorphometric analyses (inflammatory infiltrate, blood vessels, fibroblasts, eschar/epidermal thickness, IL-10 and VEGF) and biochemical assays of MPO (neutrophil), NAG (macrophage), nitrite, DCF (H 2 O 2 ), carbonyl (membrane's damage), sulfhydryl (membrane's integrity), SOD, GSH, hydroxyproline and re-epithelialization area were performed. The Injury + LED and Injury + ALA + LED groups controlled inflammation and oxidative stress, favoring angiogenesis, fibroblasts proliferation and collagen formation.

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Topical application of Azadirachta indica improves epidermal wound healing in hyperglycemic rats

Cited by 9 publications

References 45 publications

Antioxidant Efficacy of Green-Synthesized Silver Nanoparticles Promotes Wound Healing in Mice

Antioxidant Efficacy of Green-Synthesized Silver Nanoparticles Promotes Wound Healing in Mice

Cassia alata and Its Phytochemicals: A Promising Natural Strategy in Wound Recovery

Photobiomodulation and photodynamic therapy applied after electrocauterization for skin healing optimization in rats

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