Topical Anti-Inflammatory Effects of Isorhamnetin Glycosides Isolated fromOpuntia ficus-indica

Abstract

Opuntia ficus-indica (OFI) has been widely used in Mexico as a food and for the treatment of different health disorders such as inflammation and skin aging. Its biological properties have been attributed to different phytochemicals such as the isorhamnetin glycosides which are the most abundant flavonoids. Moreover, these compounds are considered a chemotaxonomic characteristic of OFI species. The aim of this study was to evaluate the effect of OFI extract and its isorhamnetin glycosides on different inflammatory markers in vitro and in vivo. OFI extract was obtained by alkaline hydrolysis of OFI cladodes powder and pure compounds were obtained by preparative chromatography. Nitric oxide (NO), cyclooxygenase-2 (COX-2), tumor necrosis factor- (TNF-) α, and interleukin- (IL-) 6 production were measured. NO production was tested in lipopolysaccharide-stimulated RAW 264.7 cells while in vivo studies were carried on croton oil-induced ear edema model. OFI extract and diglycoside isorhamnetin-glucosyl-rhamnoside (IGR) at 125 ng/mL suppressed the NO production in vitro (73.5 ± 4.8% and 68.7 ± 5.0%, resp.) without affecting cell viability. Likewise, IGR inhibited the ear edema (77.4 ± 5.7%) equating the indomethacin effects (69.5 ± 5.3%). Both IGR and OFI extract significantly inhibited the COX-2, TNF-α, and IL-6 production. IGR seems to be a suitable natural compound for development of new anti-inflammatory ingredient.

Evidence of Bioactive Compounds from Vernonia polyanthes Leaves with Topical Anti-Inflammatory Potential

“…In this study, the authors showed for the first time that HEVP and EAEVP have topical anti-inflammatory effect, which is possibly related to the presence of secondary compounds, since some flavonoids (rutin, luteolin, and apigenin) were identified in EAEVP and this effect may involve different mechanisms in the cutaneous inflammation [28,29]. Rutin, for example, inhibited the xilol-induced ear edema and reduced the cell migration and the levels of cytokines were also observed [30], while luteolin exhibits anti-inflammatory effects by blocking the activity of heat shock protein 90 in macrophages [31].…”

Traceability of Opuntia ficus‐indica L. Miller by ICP‐MS multi‐element profile and chemometric approach

“…14 This fruit also represents an interesting source of functional and healthy compounds, including total phenols, flavonoids, carotenoids, betalains, vitamins, free amino acids and the minerals Ca, Na, Mg, Zn, Fe, Mn and Se, 5,[15][16][17] with varying biological activities. 18,19 The identification of geographic provenience of food represents, first, a current and global issue for consumers, who show increasing interest in the traceability of food supply, and, second, an important quality parameter to bring about the protected denomination of origin for some foods. 20 Several techniques are applied to the study of food quality and traceability, such as foodomic methods, chromatographic and spectroscopic techniques, among others, [21][22][23][24][25] but multi-elemental analysis (MEA) is the most commonly used.…”

Untitled

“…The medicinal use of V. polyanthes for cicatrisation and cutaneous damage was the crucial stimulus for this research. For the first time, EEVPB and EFVPB showed topical anti-inflammatory effect, which can be related to the presence of secondary metabolites, since several compounds (3,7-dimethoxy-5,3',4'-trihydroxyflavone, piptocarphin A, 3',4'-dimethoxyluteolin, glaucolide A, 3,5-di-O-(E)-caffeoylquinic acid, 4,5-di-O-(E)-caffeoylquinic acid, luteolin, quercetin, protocatechuic acid, quercetin-3-O-β-glucoside, apigenin and isorhamnetin) have been identified in this plant 8,12 and this effect may involve different mechanisms in the cutaneous inflammation 29 .…”