Topical Anti-Inflammatory Activity of a Monofloral Honey of Mimosa scabrella Provided by Melipona marginata During Winter in Southern Brazil

Abstract

Melipona marginata is an endangered species of stingless bee from Brazil that produces honey with particular physicochemical features and a remarkable exotic flavor. To the best of our knowledge, this is the first report devoted to exploring the medicinal potential of this honey. Thus, the aim of this paper was to investigate the potential anti-inflammatory activity of honey extract from M. marginata on skin inflammation. The honey sample was classified as a monofloral honey of Mimosa scabrella. The presence of 11 phenolic compounds as kaempferol and caffeic acid was detected using the high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-UV-ESI-MS) method. The anti-inflammatory activity was measured using a 12-O-tetradecanoylphorbol-13-acetate-induced ear edema model of inflammation in mice. The topical application of the M. marginata honey extract (1.0 mg/ear) was able to reduce ear edema with an inhibitory effect of 54 ± 5%. This extract decreased the myeloperoxidase activity in 75 ± 3%, which suggests a lower leucocyte infiltration that was confirmed by histological analysis. This extract also provided a reduction of 55 ± 14% in the production of reactive oxygen species. This anti-inflammatory activity could be due to a synergic effect of the phenolic compounds identified in the honey sample. Taken together, these results open up new possibilities for the use of M. marginata honey extract in skin disorders.

THE USE OF HYDROGEL COMBINED WITH APPROPRIATE SUBSTRATE AND FERTILIZER IMPROVE QUALITY AND GROWTH PERFORMANCE OF Mimosa scabrella BENTH. SEEDLINGS

“…It might as well be used for civil construction, ornamentation and recovery of disturbed areas (LORENZI, 2002). An interesting novel application concerns the honey produced by the bee Melipona marginata, which was proved to be originated exclusively from M. scabrella flowers and showed anti-inflammatory principles (BORSATO et al, 2014).…”

Stingless bee honey and its potential value: a systematic review

“…The study conducted by Borsato et al (2014) indicates that stingless bee honey extract has a topical anti-inflammatory activity by reducing the production of reactive oxygen species, leukocyte migration and as well as reduced edema. From the study, they are comparing the correlation between the presence of the phenolic compound in the stingless bee honey with the antiinflammatory effects.…”

“…The studies conducted by Borsato et al (2014) proved that phenolic acid from an extract of honey from stingless bee had decreased the production of reactive oxygen species (ROS) in 55 ± 14%. A study done by Larrosa et al (2009) is the early report described the anti-inflammatory activity of honey extract from stingless bee species (M. marginata) by in vivo approaches thus, conclude that the stingless bee honey able to decrease ear edema (Borsato et al, 2014).…”

“…Previous study showed that stingless bee honey can act as anti-inflammatory (Borsato et al, 2014), anticancer (Kustiawan et al, 2014;Yazan et al, 2016), antimicrobial (Miorin et al, 2003;Demera et al, 2004;Garedew et al, 2004;Temaru et al, 2007;Kimoto-Nira and Amano, 2008;Chanchao et al, 2009;Boorn et al, 2010;Rodríguez et al, 2012;Ilechie et al, 2012;Andualem, 2013;Ewnetu et al, 2013;Mercês et al, 2013;Queiroz et al, 2013;Zainol et al, 2013;Nobre da Cruz et al, 2014;Massaro et al, 2014;Zamora et al, 2014;Medeiros et al, 2016;De Sousa et al, 2016; and possessed antioxidant properties (Duarte et al, 2012;Almeida da Silva et al, 2013;De Sousa et al, 2016). However, the beneficial of stingless bee honey has been abandoned in modern medicine due to the paucity of systematic scientific studies for supporting its medical properties (Pe´rez et al, 2006).…”

“…The color of the honey is usually clearer, liquid and has a sweet and sour taste (Roowi et al, 2012). The recent studies showed that the stingless bee honey has the potential to treat colorectal cancer (Yazan et al, 2016), anti-inflammatory (Borsato et al, 2014), antimicrobial (Zainol et al, 2013;Nobre da Cruz et al, 2014;Massaro et al, 2014;Medeiros et al, 2016;De Sousa et al, 2016; and has an antioxidant property (Almeida Da Silva et al, 2013;Duarte et al, 2012).…”