Topical administration of hydroethanolic extract of Lawsonia inermis (henna) accelerates excisional wound healing process by reducing tissue inflammation and amplifying glucose uptake

Abstract

Several studies have reported the beneficial effects of Lawsonia inermis on wound healing, but the mechanism of action is still unknown. This study aimed to investigate the effectiveness of a new ointment formulation of hydroethanolic extract leaves of L. inermis on wound healing by gene expression of glucose transporter‐1 (Glut‐1) and insulin‐like growth factor I (Igf‐1) in Wistar rats. The animals were topically treated with different doses of L. inermis. An experimentally induced circular excisional wound model of 314 mm2 surface area was surgically created. The percentage of wound contraction and histopathological changes was assessed at different time points following wound induction. The expression of Glut‐1 and Igf‐1 was evaluated by reverse‐transcription PCR. Topical administration of L. inermis, dose dependently, shortened inflammatory phase, accelerated cellular proliferation, and enhanced wound contraction ratio. It also improved revascularization, collagen deposition, and re‐epithelialization rate and promoted intracytoplasmic carbohydrate storage (P < 0.05). Moreover, the mRNA levels of Igf‐1 and Glut‐1 were significantly higher in the L. inermis‐treated groups than the control group (P < 0.05). Topical administration of L. inermis promoted the healing process by reducing tissue inflammation and increasing glucose uptake, which was mediated by up‐regulating the expression of Igf‐1 and Glut‐1.

Topical application of Cinnamon verum essential oil accelerates infected wound healing process by increasing tissue antioxidant capacity and keratin biosynthesis

“…On the other hand, antioxidants involve in wound healing by reducing inflammatory phase . The inflammation phase is main stage in removing the cellular debris from tissue and extensive response to microbial infection . Our in vitro studies showed that cinnamon essential oil has antibacterial activity against S aureus and P aeruginosa .…”

“…in vitro studies have shown that IGF‐1 not only increases proliferation and fibroblast cells, but also provokes transport glucose in short‐term during wound healing process . IGF‐1 signaling upregulates the glucose uptake through cell membrane by increasing the GLUT‐1 expression . Semenova et al showed that, overexpression of locally acting IGF‐1 under the K14 promoter enhances murine healing .…”

“…It is accepted the role of some proto‐oncogenes, interleukins, as well as growth factors in accelerating the healing process. Growth factor similar to insulin or insulin‐like growth factor I (IGF‐1) is a cytokine, which participates in the cellular granulation process during wound healing . IGF‐1 not only influences growth but also stimulates DNA and protein synthesis, cell proliferation and glucose transport .…”

“…Growth factor similar to insulin or insulin‐like growth factor I (IGF‐1) is a cytokine, which participates in the cellular granulation process during wound healing . IGF‐1 not only influences growth but also stimulates DNA and protein synthesis, cell proliferation and glucose transport . Decreased endothelial insulin/IGF‐1 signaling directly leads to faulted wound healing in mice …”

“…Herbal remedies are used for wound healing. However, the exact mechanisms of medicinal plants on wound healing have not been elucidated . Cinnamomum verum (cinnamon), a common spice from the Lauraceae family, is obtained from the inner bark of trees from the genus Cinnamomum has medicinal or culinary uses .…”